{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:58:07Z","timestamp":1761631087362,"version":"build-2065373602"},"reference-count":92,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In cattle, the transfer of passive immunity from mother to calf across the placenta is limited. Therefore, providing quality colostrum or supplementing with additives that enhance this attribute is crucial to optimise the transfer of passive immunity (TPI). The objective of the present study was to investigate the effect of capsaicin on the absorption of immunoglobulins, metabolites, and enzymes to optimise TPI in neonatal calves, and to assess its impact on selected physiological parameters. Two experimental groups were established: a control group (CON; n = 8), which received colostrum in three feedings (at 1, 12, and 20 h after calving), and a capsaicin group (CAP; n = 8) which received 40 mg of capsaicin per kilogram of body weight added to the colostrum. Birth weight (CON = 36.7 \u00b1 1.10 kg; CAP = 36.2 \u00b1 2.64 kg) and weight at 48 h of age (CON = 38.5 \u00b1 1.11 kg; CAP = 38.0 \u00b1 2.82 kg) were similar between the two experimental groups. At 48 h post-colostrum feeding, the CAP group exhibited significantly higher serum concentrations of IgG, total protein, and albumin\u2014up to 23.4 times greater than those observed in the CON group (p &lt; 0.001). Moreover, levels of glucose, cholesterol, and triglycerides were significantly elevated in the CAP group compared to the CON group (p &lt; 0.05). Similarly, higher concentrations of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase were observed in the CAP group. These findings suggest that the inclusion of 40 mg of capsaicin per kilogram of body weight in colostrum is safe and contributes positively to improving TPI in crossbred Holstein calves raised at altitudes higher than 2500 m above sea level.<\/jats:p>","DOI":"10.3390\/ani15121676","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T06:11:08Z","timestamp":1749190268000},"page":"1676","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Influence of Capsaicin Supplementation on the Enhancement of Passive Immunity Transfer Through Modulation of Immunoglobulin Absorption in Neonatal Calves"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9301-4287","authenticated-orcid":false,"given":"Ermes R.","family":"Rodas","sequence":"first","affiliation":[{"name":"Faculty of Agriculture Sciences, University of Cuenca, Cuenca 010107, Ecuador"},{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid (UCM), Avda Pta. de Hierro s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6543-7594","authenticated-orcid":false,"given":"Luis E.","family":"Ayala","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture Sciences, University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-9028","authenticated-orcid":false,"given":"Jorge B.","family":"Dut\u00e1n","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture Sciences, University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0751-8895","authenticated-orcid":false,"given":"Gissela E.","family":"Ga\u00f1an","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture Sciences, University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5669-6014","authenticated-orcid":false,"given":"Jos\u00e9 L.","family":"Pes\u00e1ntez","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture Sciences, University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan V.","family":"Gonz\u00e1lez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid (UCM), Avda Pta. de Hierro s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Szenci, O. (2022). Accuracy to Predict the Onset of Calving in Dairy Farms by Using Different Precision Livestock Farming Devices. Animals, 12.","DOI":"10.3390\/ani12152006"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.3390\/ani3041036","article-title":"Why Do So Many Calves Die on Modern Dairy Farms and What Can We Do about Calf Welfare in the Future?","volume":"3","author":"Mee","year":"2013","journal-title":"Animals"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.cvfa.2004.06.005","article-title":"Managing the production, storage, and delivery of colostrum","volume":"20","author":"McGuirk","year":"2004","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"451","DOI":"10.3168\/jds.S0022-0302(80)82952-3","article-title":"Effect of Suckling Followed by Bottle Feeding Colostrum on Immunoglobulin Absorption and Calf Survival","volume":"63","author":"Brignole","year":"1980","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"420","DOI":"10.2460\/javma.237.4.420","article-title":"Effect of colostral volume, interval between calving and first milking, and photoperiod on colostral IgG concentrations in dairy cows","volume":"237","author":"Morin","year":"2010","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.3168\/jds.S0022-0302(07)71614-4","article-title":"An observational analysis of twin births, calf sex ratio, and calf mortality in Holstein dairy cattle","volume":"90","author":"Stewart","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.cvfa.2019.07.005","article-title":"Colostrum management for dairy calves","volume":"35","author":"Godden","year":"2019","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Puppel, K., Go\u0142\u0119biewski, M., Grodkowski, G., Sl\u00f3sarz, J., Kunowska-Sl\u00f3sarz, M., Solarczyk, P., \u0141ukasiewicz, M., Balcerak, M., and Przysucha, T. (2019). Composition and factors affecting quality of bovine colostrum: A review. Animals, 9.","DOI":"10.3390\/ani9121070"},{"key":"ref_9","first-page":"396","article-title":"Factors of influence on the quality of colostrum from dairy cows","volume":"83","author":"Scholz","year":"2011","journal-title":"Z\u00fcchtungskunde"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"419","DOI":"10.2460\/javma.1991.198.03.419","article-title":"Comparison of three methods of feeding colostrum to dairy calves","volume":"198","author":"Besser","year":"1991","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6608","DOI":"10.3168\/jds.2013-7571","article-title":"Effect of the mass of immunoglobulin (Ig)G intake and age at first colostrum feeding on serum IgG concentration in Holstein calves","volume":"97","author":"Osaka","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_12","unstructured":"Laestander, C. (2016). Comparison of Three Different Colostrum Feeding Methods on Passive Transfer of Immunity, Growth and Health in Dairy Calves. [Master\u2019s Thesis, Swedish University of Agricultural Sciences]. Available online: https:\/\/stud.epsilon.slu.se\/9018\/7\/laestander_c_160513.pdf."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"420","DOI":"10.15232\/S1080-7446(15)31240-7","article-title":"Case study: Effects of colostrum ingestion on lactational performance","volume":"21","author":"Faber","year":"2005","journal-title":"Prof. Anim. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5536","DOI":"10.3168\/jds.2010-3253","article-title":"The influence of colostral immunoglobulin concentration in heifer calves\u2019 serum on their health and growth","volume":"94","author":"Rzasa","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.prevetmed.2018.01.009","article-title":"Association between passive immunity and health status of dairy calves under 30 days of age","volume":"152","author":"Lora","year":"2018","journal-title":"Prev. Vet. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1758","DOI":"10.3168\/jds.2008-1847","article-title":"Improving passive transfer of immunoglobulins in calves. II: Interaction between feeding method and volume of colostrum fed","volume":"92","author":"Godden","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10266","DOI":"10.3168\/jds.2019-16815","article-title":"Associations between colostrum management, passive immunity, calf-related hygiene practices, and rates of mortality in preweaning dairy calves","volume":"102","author":"Barry","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3713","DOI":"10.3168\/jds.2009-2943","article-title":"An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle","volume":"93","author":"Bielmann","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.3168\/jds.2015-10257","article-title":"Short communication: Intake, milk production, and milk fatty acid profile of dairy cows fed diets combining fresh forage with a total mixed ration","volume":"99","author":"Mendoza","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.3168\/jds.S0022-0302(05)72933-7","article-title":"Preventing bacterial contamination and proliferation during the harvest, storage, and feeding of fresh bovine colostrum","volume":"88","author":"Stewart","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.idairyj.2018.05.016","article-title":"Colostrum immunoglobulins: Processing, preservation and application aspects","volume":"85","author":"Borad","year":"2018","journal-title":"Int. Dairy J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/apt.14157","article-title":"Systematic review: Exercise-induced gastrointestinal syndrome\u2014Implications for health and intestinal disease","volume":"46","author":"Costa","year":"2017","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.3168\/jds.S0022-0302(06)72281-0","article-title":"Heat treatment of bovine colostrum. I: Effects of temperature on viscosity and immunoglobulin G level","volume":"89","author":"McMartin","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_24","unstructured":"Ariton, A.M., Neculai-V\u0103leanu, A.S., Radu, C., Porosnicu, I., and Ungureanu, E. (2023). Bovine colostrum management and the factors influencing its quality. Sci Pap Anim Sci Biotechnol., 56, Available online: https:\/\/www.researchgate.net\/publication\/380922293_Bovine_Colostrum_Management_and_the_Factors_Influencing_its_Quality."},{"key":"ref_25","unstructured":"USDA, APHIS, VS, and CEAH (2025, January 11). Passive Transfer Status of Heifer Calves on U.S. Dairies, 1991\u20132007., Available online: https:\/\/www.aphis.usda.gov\/sites\/default\/files\/dairy07_is_passivetrans.pdf."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3973","DOI":"10.3168\/jds.2009-2225","article-title":"Prevalence of failure of passive transfer of immunity in newborn heifer calves and associated management practices on US dairy operations","volume":"92","author":"Beam","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9185","DOI":"10.3168\/jds.2017-14008","article-title":"Preweaned heifer management on US dairy operations: Part II. Factors associated with colostrum quality and passive transfer status of dairy heifer calves","volume":"101","author":"Shivley","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/00480169.2016.1256794","article-title":"A survey of antimicrobial use in dairy cows from farms in four regions of New Zealand","volume":"65","author":"Bryan","year":"2017","journal-title":"N. Z. Vet. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"567","DOI":"10.3168\/jds.2018-15070","article-title":"Using serum and plasma samples to assess failure of transfer of passive immunity in dairy calves","volume":"102","author":"Elsohaby","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"914","DOI":"10.2460\/javma.228.6.914","article-title":"Association of neonatal serum immunoglobulin G1 concentration with health and performance in beef calves","volume":"228","author":"Dewell","year":"2006","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2298\/AVB0501003S","article-title":"Thyroid gland hormones in newborn calves treated with clinoptilolite receiving different amounts of colostrum","volume":"55","year":"2005","journal-title":"Acta Vet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2298\/AVB0501011F","article-title":"The effect of a clinoptilolite based mineral adsorber on concentrations of immunoglobulin G in the serum of newborn calves fed different amounts of colostrum","volume":"55","year":"2005","journal-title":"Acta Vet."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5665","DOI":"10.3168\/jds.2007-0348","article-title":"Selenium addition to colostrum increases immunoglobulin G absorption by newborn calves","volume":"90","author":"Kamada","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.3168\/jds.S0022-0302(95)76780-7","article-title":"Concentrations of Trypsin Inhibitor and Immunoglobulins in Colostrum of Jersey Cows","volume":"78","author":"Quigley","year":"1995","journal-title":"J. Dairy Sci."},{"key":"ref_35","first-page":"1","article-title":"Antimicrobianos en el calostro bovino","volume":"73","year":"2015","journal-title":"Entorno Ganad."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Su, M., She, Y., Deng, M., Guo, Y., Li, Y., Liu, G., Zhang, H., Sun, B., and Liu, D. (2023). The effect of capsaicin on growth performance, antioxidant capacity, immunity, and gut micro-organisms of calves. Animals, 13.","DOI":"10.3390\/ani13142309"},{"key":"ref_37","first-page":"1","article-title":"Effect of capsaicin on the barrier functions of porcine intestinal epithelial cells (IPEC-J2)","volume":"4","author":"Espana","year":"2021","journal-title":"J. Pharmacol. Pharmaceut. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.3168\/jds.2006-644","article-title":"Invited review: Essential oils as modifiers of rumen microbial fermentation","volume":"90","author":"Calsamiglia","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1271\/bbb.66.532","article-title":"Studies on the Antimicrobial Mechanisms of Capsaicin Using Yeast DNA Microarray","volume":"66","author":"Kurita","year":"2002","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0165-6147(02)01999-5","article-title":"The diversity in the vanilloid (TRPV) receptor family of ion channels","volume":"23","author":"Gunthorpe","year":"2002","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S1471-4892(02)00149-2","article-title":"Hot channels in airways: Pharmacology of the vanilloid receptor","volume":"2","author":"Hwang","year":"2002","journal-title":"Curr. Opin. Pharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"69","DOI":"10.30972\/vet.2811556","article-title":"Estr\u00e9s en ganado: Causas y consecuencias","volume":"28","author":"Romera","year":"2017","journal-title":"Rev. Vet."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Basith, S., Cui, M., Hong, S., and Choi, S. (2016). Harnessing the Therapeutic Potential of Capsaicin and Its Analogues in Pain and Other Diseases. Molecules, 21.","DOI":"10.3390\/molecules21080966"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/BF01460014","article-title":"Responses of calves to a simulated altitude of 5000 m","volume":"19","author":"Bianca","year":"1975","journal-title":"Int. J. Biometeorol."},{"key":"ref_45","unstructured":"World Organisation for Animal Health (OIE) (2016, January 22\u201327). 84th General Session: Final Report. Proceedings of the 84th General Session, Paris, France. Available online: https:\/\/www.woah.org\/app\/uploads\/2021\/03\/a-fr-2016-public.pdf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3168\/jds.S0022-0302(89)79081-0","article-title":"A Body Condition Scoring Chart for Holstein Dairy Cows","volume":"72","author":"Edmonson","year":"1989","journal-title":"J. Dairy Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"10151","DOI":"10.3168\/jds.2017-13062","article-title":"A 100-Year Review: Calf nutrition and management","volume":"100","author":"Kertz","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_48","unstructured":"NRC (2001). Nutrient Requirements of Dairy Cattle, National Academy Press. [7th ed.]."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.3168\/jds.S0022-0302(87)80235-7","article-title":"Characteristics of Frozen Colostrum Thawed in a Microwave Oven","volume":"70","author":"Jones","year":"1987","journal-title":"J. Dairy Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.tvjl.2013.06.007","article-title":"Newborn calf vitality: Risk factors, characteristics, assessment, resulting outcomes and strategies for improvement","volume":"198","author":"Murray","year":"2013","journal-title":"Vet. J."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Abd El-Fattah, A.M., Abd Rabo, F.H.R., El-Dieb, S.M., and Elkashef, H.A.S. (2012). Changes in composition of colostrum of Egyptian buffaloes and Holstein cows. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-19"},{"key":"ref_52","first-page":"51","article-title":"Influence of feto-pelvic disproportion on milk cows fertility","volume":"1","author":"Gehrke","year":"2018","journal-title":"Transl. Res. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cvfa.2007.10.002","article-title":"Newborn dairy calf management","volume":"24","author":"Mee","year":"2008","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1439-0531.2008.01123.x","article-title":"Factors Affecting Dystocia in Cattle","volume":"44","author":"Zaborski","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3688","DOI":"10.3168\/jds.2023-24164","article-title":"The Association Between Calf Birth Weight and the Postcalving Performance of Its Dairy Dam in the Absence of Dystocia","volume":"107","author":"Condon","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_56","first-page":"87","article-title":"Calf Birth Weight and Its Association with Dam\u2019s Subsequent Lactation Performance and Lactation Curve in Holstein Dairy Cows in Iran","volume":"22","author":"Asaadi","year":"2019","journal-title":"Arch. Zootech."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.3168\/jds.S0022-0302(81)82754-3","article-title":"Colostral Immunoglobulin Concentrations Among Breeds of Dairy Cattle","volume":"64","author":"Muller","year":"1981","journal-title":"J. Dairy Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.3168\/jds.S0022-0302(91)78361-6","article-title":"Hypoxia in neonatal calves: Effect on intestinal transport of immunoglobulins","volume":"74","author":"Tyler","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"100036","DOI":"10.1016\/j.anopes.2022.100036","article-title":"Clinical physiological parameters of Holstein calves in the first month of life","volume":"2","author":"Santos","year":"2023","journal-title":"Anim. Open Space"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1080\/23328940.2015.1048928","article-title":"Effect of capsaicin on thermoregulation: An update with new aspects","volume":"2","year":"2015","journal-title":"Temperature"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1080\/23328940.2015.1040604","article-title":"TRP ion channels in thermosensation, thermoregulation and metabolism","volume":"2","author":"Wang","year":"2015","journal-title":"Temperature"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Szenci, O. (2023). Importance of monitoring fetal and neonatal vitality in bovine practices. Animals, 13.","DOI":"10.3390\/ani13061081"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1139\/cjas-2020-0149","article-title":"Effects of colostrum management on transfer of passive immunity and the potential role of colostral bioactive components on neonatal calf development and metabolism","volume":"101","author":"Lopez","year":"2021","journal-title":"Can. J. Anim. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1293\/tox.2013-0060","article-title":"A comparison of the histological structure of the placenta in experimental animals","volume":"27","author":"Furukawa","year":"2014","journal-title":"J. Toxicol. Pathol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cvfa.2007.10.005","article-title":"Colostrum Management for Dairy Calves","volume":"24","author":"Godden","year":"2008","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.vetimm.2008.10.300","article-title":"The neonatal Fc receptor plays a crucial role in the metabolism of IgG in livestock animals","volume":"128","author":"Cervenak","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0041-008X(84)90121-2","article-title":"Gastrointestinal absorption and metabolism of capsaicin and dihydrocapsaicin in rats","volume":"72","author":"Kawada","year":"1984","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.lfs.2008.04.018","article-title":"Capsaicin-sensitive intestinal mucosal afferent mechanism and body fat distribution","volume":"83","author":"Leung","year":"2008","journal-title":"Life Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jtemb.2012.11.003","article-title":"Enhanced intestinal uptake of iron, zinc and calcium in rats fed pungent spice principles\u2014Piperine, capsaicin and ginger (Zingiber officinale)","volume":"27","author":"Prakash","year":"2013","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/j.1439-0442.1997.tb01139.x","article-title":"Effects of feeding colostrum, glucose or water on the first day of life on plasma immunoglobulin G concentrations and \u03b3-glutamyltransferase activities in calves","volume":"44","author":"Hadorn","year":"1997","journal-title":"J. Vet. Med. A Physiol. Pathol. Clin. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/vcp.12218","article-title":"Biochemical variables from Holstein-Friesian calves older than one week are comparable to those obtained from adult animals of stable metabolic status on the same farm","volume":"44","author":"Castillo","year":"2015","journal-title":"Vet. Clin. Pathol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"6430","DOI":"10.3168\/jds.2017-13553","article-title":"Technical note: Serum total protein and immunoglobulin G concentrations in neonatal dairy calves over the first 10 days of age","volume":"101","author":"Wilm","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.3168\/jds.2014-7939","article-title":"Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves","volume":"97","author":"Deelen","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Li, Z., Zhang, J., Wang, T., Zhang, J., Zhang, L., and Wang, T. (2022). Effects of capsaicin on growth performance, meat quality, digestive enzyme activities, intestinal morphology, and organ indexes of broilers. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.841231"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1590\/S0100-736X2012001000005","article-title":"Transfer of Passive Immunity and Serum Proteinogram in the First Six Months of Life of Criollo Lageano and Black and White Holstein Calves","volume":"32","author":"Teixeira","year":"2012","journal-title":"Veterin\u00e1ria Bras."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.rvsc.2006.10.017","article-title":"Hematology and serum biochemistry of Holstein dairy calves: Age related changes and comparison with blood composition in adults","volume":"83","author":"Mohri","year":"2007","journal-title":"Res. Vet. Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1439-0442.1998.tb00806.x","article-title":"Clinical, haematological, metabolic and endocrine traits during the first three months of life of suckling Simmentaler calves held in a cow-calf operation","volume":"45","author":"Egli","year":"1998","journal-title":"J. Vet. Med. A Physiol. Pathol. Clin. Med."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.domaniend.2012.02.005","article-title":"Energy Metabolism in the Newborn Farm Animal with Emphasis on the Calf: Endocrine Changes and Responses to Milk-Born and Systemic Hormones","volume":"43","author":"Hammon","year":"2012","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3945\/jn.110.128652","article-title":"Intestinal glucose absorption but not endogenous glucose production differs between colostrum- and formula-fed neonatal calves","volume":"141","author":"Junghans","year":"2011","journal-title":"J. Nutr."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1093\/jn\/127.10.2024","article-title":"Delaying colostrum intake by one day impairs plasma lipid, essential fatty acid, carotene, retinol and alpha-tocopherol status in neonatal calves","volume":"127","author":"Blum","year":"1997","journal-title":"J. Nutr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.domaniend.2005.11.004","article-title":"Effects of Colostrum Feeding and Glucocorticoid Administration on Insulin-Dependent Glucose Metabolism in Neonatal Calves","volume":"31","author":"Scheuer","year":"2006","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0301-6226(00)00222-0","article-title":"Colostrum effects on the gastrointestinal tract, and on nutritional, endocrine and metabolic parameters in neonatal calves","volume":"66","author":"Blum","year":"2000","journal-title":"Livest. Prod. Sci."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1093\/jn\/127.10.2011","article-title":"Nutrient metabolism: Delaying colostrum intake by one day has important effects on metabolic traits and on gastrointestinal and metabolic hormones in neonatal calves","volume":"127","author":"Hadorn","year":"1997","journal-title":"J. Nutr."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"609","DOI":"10.2527\/2000.783609x","article-title":"Growth performance, metabolic and endocrine traits, and absorptive capacity in neonatal calves fed either colostrum or milk replacer at two levels","volume":"78","author":"Kuhne","year":"2000","journal-title":"J. Anim. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1017\/S0021859600018645","article-title":"Plasma lipids of newborn and adult ruminants and of lambs from birth to weaning","volume":"69","author":"Leat","year":"1967","journal-title":"J. Agric. Sci. Camb."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1186\/BF03547951","article-title":"Serum enzyme activity of newborn calves, pigs and lambs","volume":"11","author":"Tollersrud","year":"1970","journal-title":"Acta Vet. Scand."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Yu, K., Canalias, F., Sol\u00e0-Oriol, D., Arroyo, L., Pato, R., Saco, Y., Terr\u00e9, M., and Bassols, A. (2019). Age-related serum biochemical reference intervals established for unweaned calves and piglets in the post-weaning period. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00123"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.3168\/jds.S0022-0302(91)78383-5","article-title":"Carbohydrate, enzyme, and hematology dynamics in newborn calves","volume":"74","author":"Kurz","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1093\/jn\/128.3.624","article-title":"Metabolic and endocrine traits of neonatal calves are influenced by feeding colostrum for different durations or only milk replacer","volume":"128","author":"Hammon","year":"1998","journal-title":"J. Nutr."},{"key":"ref_90","unstructured":"Kaneko, J.J., Harvey, J.W., and Bruss, M. (2008). Clinical Biochemistry of Domestic Animals, Academic Press. [6th ed.]. Available online: https:\/\/www.scirp.org\/reference\/referencespapers?referenceid=3706450."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1046\/j.1439-0442.2001.00338.x","article-title":"Activities of gamma-glutamyltransferase, alkaline phosphatase and aspartate-aminotransferase in colostrum, milk and blood plasma of calves fed first colostrum at 0-2, 6-7, 12-13 and 24-25 h after birth","volume":"48","author":"Zanker","year":"2001","journal-title":"J. Vet. Med. A Physiol. Pathol. Clin. Med."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.2527\/jas1983.5661427x","article-title":"Effect of breed, diet and sex on the alkaline phosphatase activity in walls of the bovine rumen and abomasum","volume":"56","author":"Fay","year":"1983","journal-title":"J. Anim. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/12\/1676\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:47:31Z","timestamp":1760032051000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/12\/1676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,6]]},"references-count":92,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["ani15121676"],"URL":"https:\/\/doi.org\/10.3390\/ani15121676","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,6,6]]}}}