{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:32:36Z","timestamp":1790148756720,"version":"4.0.1"},"reference-count":40,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFC2300800"],"award-info":[{"award-number":["2021YFC2300800"]}]},{"name":"National Key Research and Development Program of China","award":["2021YFC2300802"],"award-info":[{"award-number":["2021YFC2300802"]}]},{"name":"National Key Research and Development Program of China","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"National Key Research and Development Program of China","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["2021YFC2300800"],"award-info":[{"award-number":["2021YFC2300800"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["2021YFC2300802"],"award-info":[{"award-number":["2021YFC2300802"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]},{"name":"Special Research Fund of Shanxi Agricultural University for High-level Talents","award":["2021YFC2300800"],"award-info":[{"award-number":["2021YFC2300800"]}]},{"name":"Special Research Fund of Shanxi Agricultural University for High-level Talents","award":["2021YFC2300802"],"award-info":[{"award-number":["2021YFC2300802"]}]},{"name":"Special Research Fund of Shanxi Agricultural University for High-level Talents","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"Special Research Fund of Shanxi Agricultural University for High-level Talents","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Toxoplasma gondii is one of the most widely distributed intracellular parasites worldwide, which can infect humans and a wide range of warm-blooded animals including sheep, with felines serving as its definitive host. T. gondii infection in sheep can lead to premature births, abortions and stillbirths, causing significant economic losses to the sheep industry. Sheep farming has become a key pillar of the agricultural economy in Shanxi Province, North China, but little is known about T. gondii infection in sheep in this province. In the present study, a total of 755 sheep tissue samples (682 muscle tissue samples and 73 lymphatic tissue samples) were collected from different markets in 10 different cities of Shanxi Province. The genomic DNA of all samples was extracted and the B1 gene of T. gondii was amplified by PCR. The B1 gene-positive samples were genotyped at 12 genetic markers employing the multilocus nested PCR-restriction fragment length polymorphism (Mn-PCR-RFLP). The molecular prevalence of T. gondii infection in sheep tissues in Shanxi Province was 20.5% (155\/755). The T. gondii genotype ToxoDB#9 was identified in one positive T. gondii sample, with complete genotyping at all 12 genetic markers based on Mn-PCR-RFLP. This is the first report of molecular prevalence and genotype of T. gondii infection in sheep in Shanxi Province. These results reveal the widespread distribution of T. gondii in sheep in Shanxi, which is of significant public health importance.<\/jats:p>","DOI":"10.3390\/ani15121685","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T09:43:14Z","timestamp":1749202994000},"page":"1685","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Prevalence and Genotyping of Toxoplasma gondii in Sheep Tissues Intended for Human Consumption in Shanxi Province, North China"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1528-3904","authenticated-orcid":false,"given":"Xin-Sheng","family":"Lu","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3724-0776","authenticated-orcid":false,"given":"Xiao-Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Long","family":"Yi","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6869-9203","authenticated-orcid":false,"given":"Ze-Xuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7621-4628","authenticated-orcid":false,"given":"Wen-Bin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":false,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: Global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"Pappas","year":"2009","journal-title":"Int. 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