{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:12:43Z","timestamp":1776125563210,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Aldosterone, a mineralocorticoid hormone synthesised in the adrenal cortex, is essential for maintaining electrolyte balance and fluid homeostasis. Its role in feline physiology remains underexplored, despite its importance in regulating sodium reabsorption and potassium excretion via mineralocorticoid receptors in renal tubules. This study is warranted given aldosterone\u2019s importance in cats, particularly in light of their unique physiological traits, including highly concentrated urine and sensitivity to hydration status. Primary hyperaldosteronism, the most common feline adrenocortical disorder, contributes to arterial hypertension and chronic kidney disease, yet often remains underdiagnosed due to overlapping symptoms like hypokalaemia and hypertension. This research aimed to validate a liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS) method to measure serum aldosterone and to establish a reference interval in a population of healthy cats across a broad age range. The method demonstrated high precision and accuracy, with inter-assay coefficients of variation under 15%. Analysis of 49 healthy cats (40 young, 9 old) revealed a reference interval of 5.0\u201378.4 pg\/mL (13.8\u2013217.2 pmol\/L). These findings provide a robust framework for diagnosing aldosterone-related disorders in cats and underscore the need for species-specific diagnostic tools. Improved understanding of aldosterone\u2019s role could refine treatment strategies and enhance outcomes for affected feline patients.<\/jats:p>","DOI":"10.3390\/ani15121687","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T09:43:14Z","timestamp":1749202994000},"page":"1687","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Establishment and Validation of Sensitive Liquid Chromatography\u2013Tandem Mass Spectrometry Method for Aldosterone Quantification in Feline Serum with Reference Interval Determination"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1935-5146","authenticated-orcid":false,"given":"Tommaso","family":"Furlanello","sequence":"first","affiliation":[{"name":"San Marco Veterinary Clinic and Laboratory, Via dell\u2019Industria 3, 35030 Veggiano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca Maria","family":"Bertolini","sequence":"additional","affiliation":[{"name":"San Marco Veterinary Clinic and Laboratory, Via dell\u2019Industria 3, 35030 Veggiano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3592-0360","authenticated-orcid":false,"given":"Andrea","family":"Zoia","sequence":"additional","affiliation":[{"name":"San Marco Veterinary Clinic and Laboratory, Via dell\u2019Industria 3, 35030 Veggiano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3728-265X","authenticated-orcid":false,"given":"Jose","family":"Sanchez del Pulgar","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2192-4336","authenticated-orcid":false,"given":"Riccardo","family":"Masti","sequence":"additional","affiliation":[{"name":"San Marco Veterinary Clinic and Laboratory, Via dell\u2019Industria 3, 35030 Veggiano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1053\/j.ajkd.2022.04.016","article-title":"Aldosterone, Mineralocorticoid Receptor Activation, and CKD: A Review of Evolving Treatment Paradigms","volume":"80","author":"Epstein","year":"2022","journal-title":"Am. 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