{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T22:43:58Z","timestamp":1768344238646,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["421658\/2018-5"],"award-info":[{"award-number":["421658\/2018-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study investigates the effects of heat stress in a climate chamber from day 0 to 28 days of life on physiological responses, intake, nutrient digestibility, immunity, and performance in neonatal Holstein calves. Thirty-four calves (nineteen females, fifteen males) were randomly assigned to a control group (CON, temperature\u2013humidity index [THI] 66, with 22 \u00b0C and 65% humidity for 24 h, n = 17) or a heat-stressed (HS) group, which was exposed to a THI of 82, 32 \u00b0C, and 65% humidity for 9 h, followed by a THI of 66, 22 \u00b0C, and 65% humidity for 15 h. The HS calves exhibited increased respiratory rates and rectal temperatures (p &lt; 0.001), particularly during heat exposure periods, as well as a 59.5% increase in water intake compared to CON. While milk and solid feed intake, average daily gain, and feed efficiency were similar between groups, HS calves had reduced ether extract digestibility and altered ruminal fermentation, including lower acetate and lower propionate concentrations. The blood cytokine analysis showed elevated interleukin-4 and reduced interleukin-8 and IP-10 levels in heat-stressed calves, indicating a shift toward an anti-inflammatory immune profile. Despite no major performance impairments, heat stress has induced clear physiological, digestive, and immunological changes. These results underscore the importance of implementing thermal mitigation strategies during early life to safeguard calf health and development in increasingly warmer climates.<\/jats:p>","DOI":"10.3390\/ani15131876","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T08:23:22Z","timestamp":1750839802000},"page":"1876","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-0085","authenticated-orcid":false,"given":"Luiz F. M.","family":"Neves","sequence":"first","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6367-5562","authenticated-orcid":false,"given":"Mariana B.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7081-382X","authenticated-orcid":false,"given":"Joana P.","family":"Campolina","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5332-6010","authenticated-orcid":false,"given":"Mariana M.","family":"Campos","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation\u2014Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7133-994X","authenticated-orcid":false,"given":"Eduardo M. B.","family":"Souza","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5119-2442","authenticated-orcid":false,"given":"Jaciara","family":"Diav\u00e3o","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation\u2014Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-3159","authenticated-orcid":false,"given":"Abias S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation\u2014Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8788-5715","authenticated-orcid":false,"given":"Thierry R.","family":"Tomich","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation\u2014Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1346-5240","authenticated-orcid":false,"given":"Wanessa A.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation\u2014Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4484-7233","authenticated-orcid":false,"given":"Helena F.","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra G.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, J., Li, J., Wang, F., Xiao, J., Wang, Y., Yang, H., Li, S., and Cao, Z. 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