{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:09:16Z","timestamp":1791515356794,"version":"4.3.4"},"reference-count":36,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"STI2030\u2014Major Projects","award":["2023ZD040520102"],"award-info":[{"award-number":["2023ZD040520102"]}]},{"name":"STI2030\u2014Major Projects","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"name":"STI2030\u2014Major Projects","award":["ASTIP-2021-IAS-16"],"award-info":[{"award-number":["ASTIP-2021-IAS-16"]}]},{"name":"China Agriculture Research Systems","award":["2023ZD040520102"],"award-info":[{"award-number":["2023ZD040520102"]}]},{"name":"China Agriculture Research Systems","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"name":"China Agriculture Research Systems","award":["ASTIP-2021-IAS-16"],"award-info":[{"award-number":["ASTIP-2021-IAS-16"]}]},{"name":"National Germplasm Bank of Domestic Animals (2025)","award":["2023ZD040520102"],"award-info":[{"award-number":["2023ZD040520102"]}]},{"name":"National Germplasm Bank of Domestic Animals (2025)","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"name":"National Germplasm Bank of Domestic Animals (2025)","award":["ASTIP-2021-IAS-16"],"award-info":[{"award-number":["ASTIP-2021-IAS-16"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["2023ZD040520102"],"award-info":[{"award-number":["2023ZD040520102"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ASTIP-2021-IAS-16"],"award-info":[{"award-number":["ASTIP-2021-IAS-16"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Evaluation of semen quality is crucial for predicting fertility success in artificial insemination and eventual rooster selection within poultry breeding programs. However, the multitude of parameters obtained in semen quality analysis makes it challenging for breeders to make informed selection decisions. This study evaluated semen samples from 210 roosters in seven chicken breeds, using a computer-aided sperm analysis (CASA) system. A multivariate approach utilizing factor analysis (FA) was applied to simplify the data. The FA condensed 14 semen quality traits into 3 factors, linearity factor, velocity factor, and quantitative factor, explaining 75.82% of the original variations. The Beijing-You breed was selected to analyze the association between fertility and these semen quality factors, and we found high correlation between fertility and quantitative (r = 0.84) and linearity (r = 0.63) factors, and low correlation with the velocity factor (r = 0.19). Based on individual factor scores, breed clustering revealed distinct profiles with Houdan, Tibetan, and White Leghorn demonstrating lower sperm counts and poor linearity. Columbian Plymouth Rock exhibited high speed with poor linearity while Beijing-You, Rhode Island Red, and Barred Plymouth Rock displayed higher sperm counts with compromised speed and linearity. This study demonstrates that FA effectively consolidates multiple semen quality traits into fewer, biologically relevant factors. These findings provide valuable insights into breed-specific reproductive characteristics and offer actionable information for optimizing breeding in poultry production.<\/jats:p>","DOI":"10.3390\/ani15131906","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T05:00:37Z","timestamp":1751259637000},"page":"1906","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Factor Analysis of Semen Quality in Chicken and Its Impact on Fertility"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8117-6232","authenticated-orcid":false,"given":"Yunlei","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6425-7786","authenticated-orcid":false,"given":"Yanyan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7938-1231","authenticated-orcid":false,"given":"Aixin","family":"Ni","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4769-5271","authenticated-orcid":false,"given":"Hailai Hagos","family":"Tesfay","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2901-249X","authenticated-orcid":false,"given":"Adamu Mani","family":"Isa","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"Department of Animal Science, Usmanu Danfodiyo University, Sokoto 840104, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0153-1338","authenticated-orcid":false,"given":"Yunhe","family":"Zong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5865-1338","authenticated-orcid":false,"given":"Hui","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7993-0742","authenticated-orcid":false,"given":"Jingwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-009X","authenticated-orcid":false,"given":"Jilan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3923\/ajava.2014.211.228","article-title":"Artificial insemination in poultry and possible transmission of infectious pathogens: A review","volume":"9","author":"Dhama","year":"2014","journal-title":"Asian J. Anim. Vet. Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1017\/S0043933918000430","article-title":"History of artificial insemination in poultry, its components and significance","volume":"74","author":"Mohan","year":"2018","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"545","DOI":"10.4103\/1008-682X.154312","article-title":"The future of computer-aided sperm analysis","volume":"17","author":"Mortimer","year":"2015","journal-title":"Asian J. Androl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.21608\/epsj.2017.5605","article-title":"Analysis of sperm motility, velocity and morphometry of three egyptian indigenous chicken strains","volume":"37","author":"Sayed","year":"2017","journal-title":"Egypt. Poult. Sci. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/j.1939-4640.2003.tb02681.x","article-title":"New semen quality scores developed by principal component analysis of semen characteristics","volume":"24","author":"Agarwal","year":"2003","journal-title":"J. Androl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1071\/AN15120","article-title":"Meat quality traits of nellore bulls according to different degrees of backfat thickness: A multivariate approach","volume":"57","author":"Baldassini","year":"2016","journal-title":"Anim. Prod. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Patel, N., Bergamaschi, M., Magro, L., Petrini, A., and Bittante, G. (2019). Relationships of a detailed mineral profile of meat with animal performance and beef quality. Animals, 9.","DOI":"10.20944\/preprints201911.0025.v1"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Markos, S., Belay, B., and Dessie, T. (2024). Morphometric differentiation of three chicken ecotypes of ethiopia using multivariate analysis. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0295134"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/j.1439-0310.2010.01758.x","article-title":"Using principal components and factor analysis in animal behaviour research: Caveats and guidelines","volume":"116","author":"Budaev","year":"2010","journal-title":"Ethology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.theriogenology.2010.11.034","article-title":"Statistical series: Opportunities and challenges of sperm motility subpopulation analysis","volume":"75","author":"Tizado","year":"2011","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"889","DOI":"10.4103\/1008-682X.188448","article-title":"Sperm subpopulations in avian species: A comparative study between the rooster (gallus domesticus) and guinea fowl (numida meleagris)","volume":"18","year":"2016","journal-title":"Asian J. Androl."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Gacem, S., Valverde, A., Catalan, J., Yanez Ortiz, I., Soler, C., and Miro, J. (2021). A new approach of sperm motility subpopulation structure in donkey and horse. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.651477"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"596","DOI":"10.2164\/jandrol.05153","article-title":"Two-step cluster procedure after principal component analysis identifies sperm subpopulations in canine ejaculates and its relation to cryoresistance","volume":"27","year":"2006","journal-title":"J. Androl."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3109\/19396368.2013.766281","article-title":"Use of multivariate statistics to identify unreliable data obtained using casa","volume":"59","author":"Mendoza","year":"2013","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1177\/096228029200100105","article-title":"Principal component analysis and exploratory factor analysis","volume":"1","author":"Joliffe","year":"1992","journal-title":"Stat. Methods Med. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3382\/ps.0160019","article-title":"The collection of spermatozoa from the domestic fowl and turkey","volume":"16","author":"Burrows","year":"1937","journal-title":"Poult. Sci."},{"key":"ref_17","unstructured":"W.H.O (2021). Who laboratory manual for the examination and processing of human semen. WHO Laboratory Manual for the Examination and Processing of Human Semen, WHO."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.3382\/ps.0691764","article-title":"Analysis of poultry fertility data","volume":"69","author":"Kirby","year":"1990","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/j.1939-4640.2000.tb03277.x","article-title":"Correlation of casa velocity and linearity parameters with sperm mobility phenotype in turkeys","volume":"21","author":"King","year":"2000","journal-title":"J. Androl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"779","DOI":"10.2527\/jas.2011-4311","article-title":"Application of computer-assisted semen analysis to explain variations in pig fertility1","volume":"90","author":"Broekhuijse","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3","DOI":"10.56093\/ijans.v85i1.46113","article-title":"Computer assisted sperm analysis the relationship to bull field fertility, possible errors and their impact on outputs: A review","volume":"85","author":"Simonik","year":"2015","journal-title":"Indian J. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"949","DOI":"10.3923\/ijps.2008.949.953","article-title":"Semen quality traits of seven strains of chickens raised in the humid tropics","volume":"7","author":"Peters","year":"2008","journal-title":"Int. J. Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Alquezar-Baeta, C., Gimeno-Martos, S., Miguel-Jimenez, S., Santolaria, P., Yaniz, J., Palacin, I., Casao, A., Cebrian-Perez, J.A., Muino-Blanco, T., and Perez-Pe, R. (2019). Opencasa: A new open-source and scalable tool for sperm quality analysis. PLoS Comput. Biol., 15.","DOI":"10.1371\/journal.pcbi.1006691"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3844","DOI":"10.3382\/ps\/pex224","article-title":"Correlated responses to long-term divergent selection for 8-week body weight in female white plymouth rock chickens: Sexual maturity","volume":"96","author":"Jambui","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Liu, Y., Sun, Y., Li, Y., Bai, H., Xue, F., Xu, S., Xu, H., Shi, L., Yang, N., and Chen, J. (2017). Analyses of long non-coding rna and mrna profiling using rna sequencing in chicken testis with extreme sperm motility. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-08738-9"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.theriogenology.2018.09.010","article-title":"Identification and differential expression of micrornas in the testis of chicken with high and low sperm motility","volume":"122","author":"Liu","year":"2018","journal-title":"Theriogenology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s11250-024-03975-3","article-title":"Principal component analysis of phenotypic and breeding value data for semen traits in egyptian buffalo bulls","volume":"56","author":"Amin","year":"2024","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2147\/JMDH.S341444","article-title":"A factor analysis model for rapid evaluation of the semen quality of fertile men in china","volume":"15","author":"Wang","year":"2022","journal-title":"J. Multidiscip. Healthc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.3923\/javaa.2010.1717.1719","article-title":"The effect of spermatozoa number on fertility rate of chicken in artificial insemination programs","volume":"9","author":"Tabatabaei","year":"2010","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.5713\/ab.21.0072","article-title":"Semen evaluation: Methodological advancements in sperm quality-specific fertility assessment-a review","volume":"34","author":"Tanga","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e14742","DOI":"10.1111\/rda.14742","article-title":"Predictive indicators of cryotolerance and fertility in bovine sperm: Evaluating fresh semen quality to improveai outcomes with frozen\u2013thawed sperm","volume":"59","author":"Tamargo","year":"2024","journal-title":"Reprod. Domest. Anim."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"106194","DOI":"10.1016\/j.tvjl.2024.106194","article-title":"The contribution of different sperm parameters to better explain ram semen cryoresistance","volume":"306","author":"Tabarez","year":"2024","journal-title":"Vet. J."},{"key":"ref_33","first-page":"1269","article-title":"Comparative analysis of semen quality parameters and their relationship with fertility in different genetic groups of layer chicken","volume":"53","author":"Mavi","year":"2019","journal-title":"Indian J. Anim. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6139","DOI":"10.1016\/j.psj.2020.06.088","article-title":"Comparative studies of semen quality traits and sperm kinematic parameters in relation to fertility rate between 2 genetic groups of breed lines","volume":"99","author":"Tesfay","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.0105-6263.2004.00437.x","article-title":"Prediction of pregnancy by intrauterine insemination using casa estimates and strict criteria in patients with male factor infertility","volume":"27","author":"Shibahara","year":"2004","journal-title":"Int. J. Androl."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"452","DOI":"10.3906\/vet-1801-83","article-title":"The combination of casa kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen","volume":"42","author":"Cil","year":"2018","journal-title":"Turk. J. Vet. Anim. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/13\/1906\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:00:45Z","timestamp":1760032845000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/13\/1906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,28]]},"references-count":36,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ani15131906"],"URL":"https:\/\/doi.org\/10.3390\/ani15131906","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,28]]}}}