{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T23:31:06Z","timestamp":1783035066635,"version":"3.54.6"},"reference-count":39,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T00:00:00Z","timestamp":1751155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Program of Shandong Province","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Key Research and Development Program of Shandong Province","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Key Research and Development Program of Shandong Province","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]},{"name":"Earmarked fund for CARS","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Earmarked fund for CARS","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Earmarked fund for CARS","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The present study was conducted to investigate the effects of dietary addition of tributyrin on growth performance, antioxidant and intestinal health in weaned rabbits. Weaned Hyla rabbits (35 d, n = 1280) of similar body weight were randomly divided into four groups (eight replicates per group, 40 rabbits per replicate), fed diets with 0, 0.1%, 0.2% or 0.4% tributyrin addition. A 3-day pre-experimental period was followed by a 36-day trial period. Our results show that dietary addition of 0.2% tributyrin significantly increased the average daily gain (ADG) and final body weight (FBW) of rabbits, and decreased the diarrhea rate (p &lt; 0.05). Dietary addition of 0.1% and 0.2% tributyrin significantly increased the average carcass weight and liver weight (p &lt; 0.05). In the liver, dietary addition of tributyrin increased the content of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) compared with the control group (p &lt; 0.05). Dietary addition of 0.1% and 0.2% tributyrin significantly increased the serum GSH-PX level (p &lt; 0.05). Dietary addition of 0.2% tributyrin significantly increased the serum T-SOD level (p &lt; 0.05). Dietary addition of 0.2% tributyrin significantly increased villus height\/crypt depth (V\/C) in the duodenum (p &lt; 0.05). Dietary addition of 0.1\u20130.4% tributyrin significantly increased V\/C in the jejunum (p &lt; 0.05). Dietary addition of 0.1\u20130.2% tributyrin significantly increased the V\/C in the ileum, but significantly decreased the content of serum diamine oxidase (DAO) and of D-lactic acid in serum (p &lt; 0.05). In conclusion, dietary addition of tributyrin can improve the production performance and antioxidant ability of liver and serum, and improve the intestinal health by decreasing the diarrhea rate and the intestinal permeability and improving intestinal morphology in weaned rabbits. Based on the present results, the optimal level of tributyrin is 0.2% in the weaned rabbit diet.<\/jats:p>","DOI":"10.3390\/ani15131923","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T05:00:37Z","timestamp":1751259637000},"page":"1923","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dietary Addition of Tributyrin Improved the Production Performance, Antioxidant Ability and Intestinal Health in Weaned Rabbits"],"prefix":"10.3390","volume":"15","author":[{"given":"Nanbin","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghui","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijie","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5670-8038","authenticated-orcid":false,"given":"Fuchang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9262-2103","authenticated-orcid":false,"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.3920\/BM2017.0055","article-title":"Appropriate dose of Lactobacillus buchneri supplement improves intestinal microbiota and prevents diarrhoea in weaning Rex rabbits","volume":"9","author":"Zhou","year":"2018","journal-title":"Benef. Microbes"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"El-Sabrout, K., Sherasiya, A., Ahmad, S., Aggag, S., Nannoni, E., Cavallini, D., and Buonaiuto, G. (2024). Environmental Enrichment in Rabbit Husbandry: Comparative Impacts on Performance and Welfare. Animals, 16.","DOI":"10.3390\/ani14162367"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Abd El-Aziz, A., Elfadadny, A., Abo Ghanima, M., Cavallini, D., Fusaro, I., Giammarco, M., Buonaiuto, G., and El-Sabrout, K. (2024). Nutritional Value of Oregano-Based Products and Its Effect on Rabbit Performance and Health. Animals, 20.","DOI":"10.3390\/ani14203021"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1080\/10408398.2022.2137776","article-title":"Beneficial effects of butyrate on brain functions: A view of epigenetic","volume":"64","author":"Alpino","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.3390\/ph7111008","article-title":"Involvement of the Antioxidant Effect and Anti-inflammatory Response in Butyrate-Inhibited Vascular Smooth Muscle Cell Proliferation","volume":"7","author":"Mathew","year":"2014","journal-title":"Pharmaceuticals"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Connolly, K.R., Sweeney, T., and O\u2019Doherty, J.V. (2024). Sustainable Nutritional Strategies for Gut Health in Weaned Pigs: The Role of Reduced Dietary Crude Protein, Organic Acids and Butyrate Production. Animals, 15.","DOI":"10.20944\/preprints202412.0272.v1"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s003940070020","article-title":"Rationale for the luminal provision of butyrate in intestinal diseases","volume":"39","author":"Stein","year":"2000","journal-title":"Eur. J. Nutr."},{"key":"ref_8","first-page":"629","article-title":"Phase I study of the orally administered butyrate prodrug, tributyrin, in patients with solid tumors","volume":"4","author":"Conley","year":"1998","journal-title":"Clin. Cancer Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.2174\/138945012804545443","article-title":"Anticarcinogenic actions of tributyrin, a butyric acid prodrug","volume":"13","author":"Heidor","year":"2012","journal-title":"Curr. Drug Targets"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.psj.2020.10.006","article-title":"Rumen-bypassed tributyrin alleviates heat stress by reducing the inflammatory responses of immune cells","volume":"100","author":"Guo","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1021\/acs.jafc.8b06208","article-title":"Dietary tributyrin attenuates intestinal inflammation, enhances mitochondrial function, and induces mitophagy in piglets challenged with diquat","volume":"67","author":"Wang","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Miragoli, F., Patrone, V., Prandini, A., Sigolo, S., Dell\u2019Anno, M., Rossi, L., Senizza, A., Morelli, L., and Callegari, M.L. (2021). Implications of tributyrin on gut microbiota shifts related to performances of weaning piglets. Microorganisms, 9.","DOI":"10.3390\/microorganisms9030584"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102061","DOI":"10.1016\/j.psj.2022.102061","article-title":"Dietary tributyrin intervention improves the carcass traits, organ indices, and blood biomarker profiles in broilers under the isocaloric diets administration","volume":"101","author":"Hu","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109644","DOI":"10.1016\/j.meatsci.2024.109644","article-title":"Effects of protein grass hay as alternative feed resource on lamb\u2019s fattening performance and meat quality","volume":"218","author":"Zhang","year":"2024","journal-title":"Meat Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1093\/jas\/skz047","article-title":"Dietary addition of Artemisia argyi reduces diarrhea and modulates the gut immune function without affecting growth performances of rabbits after weaning1","volume":"97","author":"Liu","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4248","DOI":"10.1093\/jas\/skz277","article-title":"Dietary addition of garlic straw improved the intestinal barrier in rabbits1","volume":"97","author":"Liu","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.aninu.2023.08.006","article-title":"Benefits of tributyrin on growth performance, gastrointestinal tract development, ruminal bacteria and volatile fatty acid formation of weaned Small-Tailed Han lambs","volume":"15","author":"Li","year":"2023","journal-title":"Anim. Nutr."},{"key":"ref_18","first-page":"45","article-title":"A study of the growth of Pseudallescheria boydii isolates from sewage sludge and clinical sources on tributyrin, rapeseed oil, biodiesel oil and diesel oil","volume":"15","author":"Ulfig","year":"2008","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4599","DOI":"10.3168\/jds.2022-22957","article-title":"Effects of medium-chain fatty acids and tributyrin supplementation in milk replacers on growth performance, blood metabolites, and hormone concentrations in Holstein dairy calves","volume":"106","author":"Murayama","year":"2023","journal-title":"J. Dairy. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1111\/jam.15321","article-title":"Effects of dietary tributyrin and physterol ester supplementation on growth performance, intestinal morphology, microbiota and metabolites in weaned piglets","volume":"132","author":"Chen","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chen, C., Qu, M., Li, G., Wan, G., Liu, P., Omar, S.M., Mei, W., Hu, Z., Zhou, Q., and Xu, L. (2024). Dietary tributyrin improves growth performance, meat quality, muscle oxidative status, and gut microbiota in taihe silky fowls under cyclic heat stress. Animals, 14.","DOI":"10.3390\/ani14203041"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, X.E., Li, Z.W., Liu, L.L., and Ren, Q.C. (2024). Effects of supplementing tributyrin on meat quality characteristics of foreshank muscle of weaned small-tailed han. Animals, 23.","DOI":"10.3390\/ani14081235"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"620","DOI":"10.3389\/fvets.2020.620665","article-title":"Silver nanoparticles biosynthesized with salvia officinalis leaf exert protective effect on hepatic tissue injury induced by Plasmodium chabaudi","volume":"7","author":"Metwally","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2581","DOI":"10.2527\/jas.2011-4444","article-title":"Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs","volume":"90","author":"Zhu","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.aninu.2016.06.006","article-title":"Effects of dietary supplementation of Artemisia argyi aqueous extract on antioxidant indexes of small intestine in broilers","volume":"2","author":"Zhao","year":"2016","journal-title":"Anim. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7406","DOI":"10.1016\/j.aquaculture.2024.740607","article-title":"Dietary tributyrin supplementation promotes the growth by improving intestinal digestive and antioxidant capacity and the abundance of intestinal beneficial bacteria for swimming crab (Portunus trituberculatus)","volume":"583","author":"Peng","year":"2024","journal-title":"Aquaculture"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hou, J., Lian, L., Lu, L., Gu, T., Zeng, T., Chen, L., Xu, W., Li, G., Wu, H., and Tian, Y. (2023). Effects of dietary bacillus coagulans and tributyrin on growth performance, serum antioxidants, intestinal morphology, and cecal microbiota of growing yellow-feathered broilers. Animals, 13.","DOI":"10.3390\/ani13223534"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.cell.2016.05.041","article-title":"From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites","volume":"165","author":"Koh","year":"2016","journal-title":"Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1017\/S0954422410000247","article-title":"From the gut to the peripheral tissues: The multiple effects of butyrate","volume":"23","author":"Guilloteau","year":"2010","journal-title":"Nutr. Res. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wang, C., Cao, S., Shen, Z., Hong, Q., Feng, J., Peng, Y., and Hu, C. (2019). Effects of dietary tributyrin on intestinal mucosa development, mitochondrial function and AMPK-mTOR pathway in weaned pigs. J. Anim. Sci. Biotechnol., 10.","DOI":"10.1186\/s40104-019-0394-x"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5837","DOI":"10.1039\/D1FO00208B","article-title":"Dietary supplementation with Bacillus subtilis and xylo-oligosaccharides improves growth performance and intestinal morphology and alters intestinal microbiota and metabolites in weaned piglets","volume":"12","author":"Ding","year":"2021","journal-title":"Food Funct."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hu, Q., Yin, F., Li, B., Guo, Y., and Yin, Y. (2021). Dietary tributyrin administration improves intestinal morphology and selected bacterial and short-chain fatty acid profiles in broilers under an isocaloric feeding regime. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.715712"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhang, W.-X., Zhang, Y., Zhang, X.-W., Deng, Z.-X., Liu, J.-X., He, M.-L., and Wang, H.-F. (2020). Effects of Dietary Supplementation with Combination of Tributyrin and Essential Oil on Gut Health and Microbiota of Weaned Piglets. Animals, 10.","DOI":"10.3390\/ani10020180"},{"key":"ref_34","first-page":"493","article-title":"The Impact of Tributyrin on Performance and Intestinal Health of Broiler Chickens Post Coccidiosis Vaccination","volume":"3","author":"Wang","year":"2021","journal-title":"Avian Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zhao, L., Luo, L., Jia, W., Xiao, J., Huang, G., Tian, G., Li, J., and Xiao, Y. (2014). Serum diamine oxidase as a hemorrhagic shock biomarker in a rabbit model. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0102285"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7339","DOI":"10.3389\/fmedt.2021.733940","article-title":"Evaluation of fecal calprotectin, d-lactic acid and bedside gastrointestinal ultrasound image data for the prediction of acute gastrointestinal injury in sepsis patients","volume":"3","author":"Li","year":"2021","journal-title":"Front. Med. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"20596","DOI":"10.3402\/ljm.v8i0.20596","article-title":"A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia","volume":"8","author":"Cakmaz","year":"2013","journal-title":"Libyan J. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0002-9610(94)90101-5","article-title":"Serum D(-)-lactate levels as an aid to diagnosing acute intestinal ischemia","volume":"167","author":"Murray","year":"1994","journal-title":"Am. J. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chen, J., Song, Z., Ji, R., Liu, Y., Zhao, H., Liu, L., and Li, F. (2022). Chlorogenic acid improves growth performance of weaned rabbits via modulating the intestinal epithelium functions and intestinal microbiota. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1027101"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/13\/1923\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:01:28Z","timestamp":1760032888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/13\/1923"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,29]]},"references-count":39,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ani15131923"],"URL":"https:\/\/doi.org\/10.3390\/ani15131923","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,29]]}}}