{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:28:45Z","timestamp":1760059725115,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Laminosioptes cysticola (Vizioli, 1870), a tissue-dwelling mite responsible for nodular acariasis in birds, was identified from two hens reared in a rural backyard flock in Umbria, Italy. Adult mites were found in the subcutaneous tissue and on the serosal surface of various internal organs. Larval and first- and second-stage nymphal forms were observed beneath the skin and near the trachea and esophageal serosa. By comparing the existing literature with that reported in the present study, we propose a hypothetical reconstruction of the parasite\u2019s life cycle. It is postulated that the entry of L. cysticola occurs through the cervical skin, where adults mate and larviparous females give birth to larvae. These larvae migrate into the loose connective tissues surrounding the trachea and esophagus, where they develop into nymphs. The immature forms then progress along the esophagus and trachea to reach the thoracic and abdominal cavities, colonizing the serosal surfaces of visceral organs. It remains unclear whether, or how, the mites return to the subcutaneous tissues to complete their maturation. Senescent specimens degenerate within the subcutis, where they are encased by a granulomatous inflammatory reaction that leads to the formation of characteristic calcified nodules.<\/jats:p>","DOI":"10.3390\/ani15142024","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T07:38:27Z","timestamp":1752133107000},"page":"2024","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Considerations on the Life Cycle of Laminosioptes cysticola (Vizioli, 1870) Based on a Natural Infestation in Two Laying Hens"],"prefix":"10.3390","volume":"15","author":[{"given":"Iolanda","family":"Moretta","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Principato","sequence":"additional","affiliation":[{"name":"Urania Research Centre, Via del Mattatoio Vecchio 7, 06063 Magione, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3544-8812","authenticated-orcid":false,"given":"Giuseppe","family":"Giglia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-8154","authenticated-orcid":false,"given":"Elvio","family":"Lepri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Antonello","family":"Principato","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy"},{"name":"Urania Research Centre, Via del Mattatoio Vecchio 7, 06063 Magione, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"ref_1","first-page":"67","article-title":"Lista de los parasitos de los animales domesticas en la Argentina","volume":"9","author":"Roveda","year":"1947","journal-title":"Gac. Vet."},{"key":"ref_2","first-page":"87","article-title":"Une nouvelle famille d\u2019Acariens endoparasites des chauves-souris: Gastronyssidae. Fam. nov","volume":"36","author":"Fain","year":"1956","journal-title":"Ann. Soc. Belge M\u00e9d. Trop."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"78","DOI":"10.2307\/1587795","article-title":"The subcutaneous mite of chickens: An incidence report","volume":"9","author":"Cassidy","year":"1965","journal-title":"Avian Dis."},{"key":"ref_4","first-page":"37","article-title":"The parasitic fauna of poultry in Serbia. IV. The Poarevac region","volume":"19","author":"Cvetkovic","year":"1965","journal-title":"Vet. Glas."},{"key":"ref_5","first-page":"321","article-title":"Vorkommen von Laminosioptes cysticola in den Bauchorganen einer Pute [Occurrence of Laminosioptes cysticola in the abdominal organs of a hen turkey]","volume":"7","author":"Kaliner","year":"1970","journal-title":"Pathol. Vet."},{"key":"ref_6","unstructured":"Morrow, C. (1986, January 26\u201330). Poultry Parasites. Proceedings of the \u201cPoultry Health\u201d, Sydney, NSW, Australia."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1136\/vr.101.19.387","article-title":"A case of Laminosioptes cysticola infection","volume":"101","author":"Amure","year":"1977","journal-title":"Vet. Rec."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00009900","article-title":"Notes on the genus Laminosioptes Megnin, 1880 (Acari, Astigmata) with description of three new species","volume":"2","author":"Fain","year":"1981","journal-title":"Syst. Parasitol."},{"key":"ref_9","first-page":"52","article-title":"Acariasi nodulare del pollo da Laminosioptes cysticola (Acarina: Laminosioptidae): Studio micrometrico degli stadi immaturi del parassita","volume":"2","author":"Principato","year":"1991","journal-title":"Zootec. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"484","DOI":"10.2307\/1592211","article-title":"Clinical illness in a wild turkey with Laminosioptes cysticola infestation of the viscera and peripheral nerves","volume":"41","author":"Smith","year":"1997","journal-title":"Avian Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1080\/03079459994416","article-title":"Health status of free-living pigeons in the city of Santiago","volume":"28","author":"Toro","year":"1999","journal-title":"Avian Pathol."},{"key":"ref_12","first-page":"349","article-title":"Acariasis of subcutaneous and muscular tissue in poultry","volume":"56","author":"Paszowska","year":"2000","journal-title":"Med. Weter."},{"key":"ref_13","unstructured":"Reina, D., Frontera, E., S\u00e1nchez-L\u00f3pez, J., Hern\u00e1ndez, S., Navarrete, I., and Mart\u00ednez-Moreno, F.J. (2002, January 19\u201322). Fowl cyst mite (Laminosioptes cysticola) in lesser kestrel (Falco naumanni). Proceedings of the Final Conference \u201cMange and Myiasis of Livestock\u201d, Bari, Italy."},{"key":"ref_14","first-page":"66","article-title":"The diagnosis and treatment of Laminosioptes cysticola disease in Ciconia nigra","volume":"2","author":"Chen","year":"2003","journal-title":"Chin. J. Vet. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"188","DOI":"10.4102\/jsava.v80i3.200","article-title":"A survey of ectoparasites, cestodes and management of free-range indigenous chickens in rural Zimbabwe","volume":"80","author":"Mukaratirwa","year":"2009","journal-title":"Tydskr. S. Afr. Vet. Ver."},{"key":"ref_16","first-page":"10","article-title":"Parasitic infections of free-range chickens from Golestan province, Iran","volume":"4","author":"Eslami","year":"2009","journal-title":"Iran J. Parasitol."},{"key":"ref_17","first-page":"1","article-title":"Laminosioptes cysticola and Gallibacterium anatis infections in a lymphoma diseased chicken hen with a cystic right oviduct","volume":"11","author":"Fajardo","year":"2010","journal-title":"Rev. Electr\u00f3n. Vet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1590\/S0103-84782010000600038","article-title":"Laminosioptes cysticola in free-range chickens in Minas Gerais, Brazil","volume":"40","author":"Martins","year":"2010","journal-title":"Cienc. Rural"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1590\/S0103-84782012005000020","article-title":"Infesta\u00e7\u00e3o simult\u00e2nea por Cytodites nudus e Laminosioptes cysticola e seus aspectos patol\u00f3gicos em galinhas de subsist\u00eancia","volume":"42","author":"Herpich","year":"2012","journal-title":"Ci\u00eanc. Rural"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11250-011-0002-3","article-title":"Biodiversity and prevalence of parasites of domestic pigeons (Columba livia domestica) in a selected semiarid zone of South Khorasan, Iran","volume":"44","author":"Radfar","year":"2012","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_21","first-page":"94","article-title":"Prevalence and economic significance of ectoparasites infestation in village chickens (Gallus gallus domesticus) in Gombe, Northeastern Nigeria","volume":"4","author":"Lawal","year":"2016","journal-title":"Direct Res. J. Agric. Food. Sci."},{"key":"ref_22","unstructured":"Taracena Rivera, M.G. (2019). Determinaci\u00f3n de la Presencia de Laminosioptes sp. en Necropsias de Gallinas de Traspatio (Gallus gallus domesticus), Realizadas en Larrsa\u2014Usac, Guatemala, Durante el Per\u00edodo de Febrero\u2014Junio, 2018, Universidad De San Carlos De Guatemala, Facultad De Medicina Veterinaria Y Zootecnia."},{"key":"ref_23","first-page":"92","article-title":"Occurrence of ectoparasites in backyard domestic chickens (Gallus gallus domesticus) in the Northeast of Tunisia","volume":"7","author":"Kaboudi","year":"2019","journal-title":"J. Anim. Health Prod."},{"key":"ref_24","unstructured":"Heath, A., Rolfe, J., and Michel, P. (2022). Conservation Status of Parasitic Mites and Ticks (Acari) in New Zealand, 2021, Department of Conservation Te Papa Atawhai."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e460","DOI":"10.1002\/vrc2.460","article-title":"Suspected Laminosioptes cysticola (fowl cyst mite) lesions in backyard chickens in southern England","volume":"10","author":"Grist","year":"2022","journal-title":"Vet. Rec. Case Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1053\/S1055-937X(03)80003-8","article-title":"Practical Avian Necropsy","volume":"12","author":"Rae","year":"2003","journal-title":"Semin. Avian Exot. Pet Med."},{"key":"ref_27","unstructured":"Maj\u00f3, N., and Dolz, R. (2019). Atlas of Avian Necropsy: Macroscopic Diagnosis Sampling, Servet. [Update Edition]."},{"key":"ref_28","unstructured":"Krantz, G.W., and Walter, D.E. (2009). Cap. 7: Collection, Rearing, and Preparing Specimens. A Manual of Acarology, Texas Tech University Press. [3rd ed.]."},{"key":"ref_29","first-page":"391","article-title":"The description of Laminosioptes hymenopterus n. sp. (Sarcoptiformes) from the American crow","volume":"4","author":"Jones","year":"1962","journal-title":"Acarologia"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/14\/2024\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:07:17Z","timestamp":1760033237000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/14\/2024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,9]]},"references-count":29,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ani15142024"],"URL":"https:\/\/doi.org\/10.3390\/ani15142024","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,7,9]]}}}