{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T02:58:21Z","timestamp":1785725901112,"version":"3.56.0"},"reference-count":73,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"American Jersey Cattle Association","award":["GM-1071"],"award-info":[{"award-number":["GM-1071"]}]},{"name":"Ministry of Higher Education of the Arab Republic of Egypt","award":["GM-1071"],"award-info":[{"award-number":["GM-1071"]}]},{"name":"University of Wisconsin-Madison, Office of the Vice Chancellor for Research and Graduate Education","award":["GM-1071"],"award-info":[{"award-number":["GM-1071"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Sexing procedures and subsequent freezing still impact sperm cells, leading to decreased fertility of gender-ablated semen. This study aimed to enhance cryo-survivability and reduce the capacitation-like change rate of gender-ablated semen by adding 2 mg of cholesterol-loaded cyclodextrin (CLC) per mL of extended semen containing 67 \u00d7 106 sperm cells. This marks the first use of CLC with gender-ablated semen. Semen from four Jersey bulls was used for this study. Viability, motility, and mitochondrial activity were evaluated and adjusted to account for the inactivation of undesired sex sperm cells during processing. Binding ability to oviduct cells, fertilizing ability, and acrosome status were also evaluated. Adding CLC did not increase sperm motility. The population with intact membranes and acrosomes was significantly increased (p &lt; 0.05) from 28.9 \u00b1 1.2% to 34.1 \u00b1 1.2% in the CLC-treated group. Mitochondrial potential, capacitation status at the membrane, calcium levels, and binding ability to oviduct cells were maintained. CLC treatment did not delay capacitation while significantly improving fertilization rates after 8 and 12 h of co-incubation (77 \u00b1 3% vs. 67 \u00b1 3% and 82 \u00b1 3% vs. 74 \u00b1 3%, respectively; p &lt; 0.05). In conclusion, CLC addition significantly improved gender-ablated post-thaw sperm viability, acrosome integrity, and fertilizing ability while preserving motility, capacitation progress, and binding ability to oviduct cells.<\/jats:p>","DOI":"10.3390\/ani15142038","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T08:15:47Z","timestamp":1752221747000},"page":"2038","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Using Cholesterol-Loaded Cyclodextrin to Improve Cryo-Survivability and Reduce Capacitation-Like Changes in Gender-Ablated Jersey Semen"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2595-7541","authenticated-orcid":false,"given":"Ahmed S.","family":"Aly","sequence":"first","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA"},{"name":"Department of Animal Production, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Rozeboom","sequence":"additional","affiliation":[{"name":"Genus PLC & ABS Global, De Forest, WI 53532, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4085-556X","authenticated-orcid":false,"given":"John J.","family":"Parrish","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"s97","DOI":"10.1017\/S1751731118000721","article-title":"Review: Applications and Benefits of Sexed Semen in Dairy and Beef Herds","volume":"12","author":"Holden","year":"2018","journal-title":"Animal"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1017\/S1751731114000664","article-title":"Applications and Cost Benefits of Sexed Semen in Pasture-Based Dairy Production Systems","volume":"8","author":"Butler","year":"2014","journal-title":"Animal"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rahman, M.S., and Pang, M.G. 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