{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:14:44Z","timestamp":1775888084954,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Food and Agriculture Research\u2014New Investigator Award","award":["SD00H666\u201318"],"award-info":[{"award-number":["SD00H666\u201318"]}]},{"name":"USDA National Institute of Food and Agriculture","award":["SD00H666\u201318"],"award-info":[{"award-number":["SD00H666\u201318"]}]},{"name":"National Pork Board","award":["SD00H666\u201318"],"award-info":[{"award-number":["SD00H666\u201318"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Understanding protein and amino acid deposition in pregnant gilts is important for developing nutritional strategies that meet these demands and enhance reproductive performance. Current models, such as the NRC (2012) gestating sow model, assume a constant proportional protein and amino acid content in tissues throughout pregnancy. However, empirical data suggest that gestational tissue growth and composition change dynamically. In this study, we developed a gestation model that characterizes the dynamic changes in growth, crude protein, and amino acid deposition throughout gestation. Based on a systematized search of published data, mathematical functions were developed to estimate daily protein and amino acid deposition in key tissues, including allantoic and amniotic fluid, uterus, placenta, fetus, mammary gland, and maternal body. Our results suggest that dietary crude protein levels and amino acid profiles should be adjusted to meet metabolic demands, particularly in early gestation, where a potential nutritional deficiency was identified. Additionally, the amino acid profile of deposited protein shifts during late gestation, suggesting a changing demand for specific amino acids. These findings challenge existing models and highlight the need for adaptive dietary strategies that better align with pregnancy\u2019s biological demands. By refining protein and amino acid deposition estimates, this study provides a framework guiding future research on precision feeding, ultimately improving gilt and sow reproductive performance.<\/jats:p>","DOI":"10.3390\/ani15142126","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T10:10:38Z","timestamp":1752833438000},"page":"2126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterizing the Dynamic Protein and Amino Acid Deposition in Tissues of Pregnant Gilts: Implications for Stage-Specific Nutritional Strategies"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3070-3506","authenticated-orcid":false,"given":"Christian D.","family":"Ramirez-Camba","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4090-6627","authenticated-orcid":false,"given":"Pedro E.","family":"Urriola","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2978-5852","authenticated-orcid":false,"given":"Crystal L.","family":"Levesque","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD 57007, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"ref_1","unstructured":"NRC (2012). 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