{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:31:54Z","timestamp":1760059914094,"version":"build-2065373602"},"reference-count":73,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chinese National Natural Science Foundation Project","award":["31872962"],"award-info":[{"award-number":["31872962"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Hair, as a non-invasive biospecimen, retains metabolic deposits from sebaceous glands and capillaries, reflecting substances from the peripheral circulation, and provides valuable biochemical information linked to phenotypes, yet its application in animal disease research remains limited. This work applied ultra-high-performance liquid chromatography\u2013tandem mass spectrometry (UHPLC-MS\/MS) to compare the hair metabolomic characteristics of healthy forest musk deer (FMD, Moschus berezovskii) and those diagnosed with hemorrhagic pneumonia (HP), phytobezoar disease (PD), and abscess disease (AD). A total of 2119 metabolites were identified in the FMD hair samples, comprising 1084 metabolites in positive ion mode and 1035 metabolites in negative ion mode. Differential compounds analysis was conducted utilizing the orthogonal partial least squares\u2013discriminant analysis (OPLS-DA) model. In comparison to the healthy control group, the HP group displayed 85 upregulated and 92 downregulated metabolites, the PD group presented 124 upregulated and 106 downregulated metabolites, and the AD group exhibited 63 upregulated and 62 downregulated metabolites. Functional annotation using the Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the differential metabolites exhibited significant enrichment in pathways associated with cancer, parasitism, energy metabolism, and stress. Receiver operating characteristic (ROC) analysis revealed that both the individual and combined panels of differential metabolites exhibited area under the curve (AUC) values exceeding 0.7, demonstrating good sample discrimination capability. This research indicates that hair metabolomics can yield diverse biochemical insights and facilitate the development of non-invasive early diagnostic techniques for diseases in captive FMD.<\/jats:p>","DOI":"10.3390\/ani15142155","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T13:59:11Z","timestamp":1753106351000},"page":"2155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hair Metabolomic Profiling of Diseased Forest Musk Deer (Moschus berezovskii) Using Ultra-High-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry (UHPLC-MS\/MS)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8556-5734","authenticated-orcid":false,"given":"Lina","family":"Yi","sequence":"first","affiliation":[{"name":"School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5080-5861","authenticated-orcid":false,"given":"Han","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Li","sequence":"additional","affiliation":[{"name":"Feng County Xinfenghe Animal Hospital Co., Ltd., Baoji 721799, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongtao","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Tong Ren Tang Shaanxi Musk Industry Co., Ltd., Baoji 721004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6178-7431","authenticated-orcid":false,"given":"Haolin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5275-4646","authenticated-orcid":false,"given":"Defu","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"ref_1","unstructured":"Sheng, H., and Liu, Z. 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