{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:05:08Z","timestamp":1780484708752,"version":"3.54.1"},"reference-count":29,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T00:00:00Z","timestamp":1754524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["POSGRAD\/UEA 2021"],"award-info":[{"award-number":["POSGRAD\/UEA 2021"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["001\/2017"],"award-info":[{"award-number":["001\/2017"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["013\/2022"],"award-info":[{"award-number":["013\/2022"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["001\/2023"],"award-info":[{"award-number":["001\/2023"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["003\/2022"],"award-info":[{"award-number":["003\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Sporotrichosis is a zoonotic fungal infection with increasing incidence in the Brazilian Amazon, primarily affecting domestic cats and posing risks to human health. This study characterized the clinical and epidemiological profiles of 29 feline sporotrichosis cases in Manaus and optimized molecular diagnostic methods for Sporothrix species identification. Most affected cats were young (86.2% aged 1\u20133 years), male (82.7%), and free-roaming or semi-indoor (44.8% each), frequently presenting cutaneous lesions localized at the nasal planum (23.3%), ears (7%), eyes (2.3%) and other facial areas (18.6%). Three DNA extraction methods were compared; the phenol\u2013chloroform protocol yielded the highest DNA concentration and purity, and ITS1\u2013ITS4 primers showed an adequate sensibility for PCR detection. In silico RFLP profiles using common restriction enzymes showed limited discriminatory power among Sporothrix species. ITS sequencing of four high-quality amplicons confirmed all isolates as Sporothrix brasiliensis. Antifungal susceptibility testing of all isolates revealed geometric mean MICs of 0.25 \u00b5g\/mL for ketoconazole, 0.57 \u00b5g\/mL for itraconazole, 7.27 \u00b5g\/mL for amphotericin B, and 64 \u00b5g\/mL for fluconazole, respectively. These findings provide clinical, molecular, and therapeutic information supporting the diagnosis and surveillance of feline sporotrichosis in the Amazon, reinforcing the need for ongoing veterinary and public health monitoring.<\/jats:p>","DOI":"10.3390\/ani15152318","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T10:58:26Z","timestamp":1754564306000},"page":"2318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical and Molecular Characterization of Feline Sporotrichosis in the Brazilian Amazon: PCR-Based Identification of Sporothrix brasiliensis"],"prefix":"10.3390","volume":"15","author":[{"given":"Nayara F\u00e1tima","family":"Lazameth-Diniz","sequence":"first","affiliation":[{"name":"Laboratory of Mycology, Coordination of Society, Environment and Health, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Barreto de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology, Coordination of Society, Environment and Health, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via da Silva","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology, Coordination of Society, Environment and Health, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Oliveira da Silva","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology, Coordination of Society, Environment and Health, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica Simplicio de","family":"Souza","sequence":"additional","affiliation":[{"name":"School of Technology, Amazonas State University (UEA), Manaus 69065-001, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia Santana","family":"Cruz","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ani Beatriz Jackisch","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Le\u00f4nidas and Maria Deane Institute (ILMD), Oswaldo Cruz Foundation (Fiocruz Amaz\u00f4nia), Manaus 69057-070, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8967-9528","authenticated-orcid":false,"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany"},{"name":"Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 22049 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3163-7499","authenticated-orcid":false,"given":"Jo\u00e3o Vicente Braga de","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology, Coordination of Society, Environment and Health, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.3201\/eid2603.190803","article-title":"Geographic Expansion of Sporotrichosis, Brazil","volume":"26","author":"Marques","year":"2020","journal-title":"Emerg. 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