{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:39:41Z","timestamp":1787863181790,"version":"build-2784847793"},"reference-count":99,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T00:00:00Z","timestamp":1754524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2023YFD2401000"],"award-info":[{"award-number":["2023YFD2401000"]}]},{"name":"National Key R&amp;D Program of China","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"National Key R&amp;D Program of China","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund CAFS","award":["2023YFD2401000"],"award-info":[{"award-number":["2023YFD2401000"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund CAFS","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund CAFS","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]},{"name":"the earmarked fund for CARS-48-07","award":["2023YFD2401000"],"award-info":[{"award-number":["2023YFD2401000"]}]},{"name":"the earmarked fund for CARS-48-07","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"the earmarked fund for CARS-48-07","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]},{"name":"the seed industry revitalization project of Jiangsu province","award":["2023YFD2401000"],"award-info":[{"award-number":["2023YFD2401000"]}]},{"name":"the seed industry revitalization project of Jiangsu province","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"the seed industry revitalization project of Jiangsu province","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]},{"name":"Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm","award":["2023YFD2401000"],"award-info":[{"award-number":["2023YFD2401000"]}]},{"name":"Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Macrobrachium nipponense represents a commercial decapod species that predominantly inhabits freshwater ecosystems or environments with low salinity. However, the species exhibits normal survival and reproductive capacity in natural aquatic habitats with salinity levels up to 10 parts per thousand (ppt). The present study aimed to elucidate the molecular mechanisms underlying salinity acclimation in M. nipponense by investigating alterations in oxidative stress, morphological adaptations, and hepatopancreatic gene expression profiles following exposure to a salinity level of 10 ppt. The present study demonstrates that glutathione peroxidase and Na+\/K+-ATPase play critical roles in mitigating oxidative stress induced by elevated salinity in M. nipponense. Furthermore, histological analysis revealed distinct pathological alterations in the hepatopancreas of M. nipponense following 7-day salinity exposure, including basement-membrane disruption, luminal expansion, vacuolization, and a marked reduction in storage cells. Transcriptomic profiling of M. nipponense hepatopancreas suggested coordinated activation of both immune (lysosome and protein processing in endoplasmic reticulum pathways) and energy (pyruvate metabolism, glycolysis\/gluconeogenesis, and citrate cycle) metabolic processes during salinity acclimation in M. nipponense. Quantitative real-time PCR validation confirmed the reliability of RNA-seq data. This study provides molecular insights into the salinity adaptation mechanisms in M. nipponense, offering potential applications for improving cultivation practices in brackish water environments.<\/jats:p>","DOI":"10.3390\/ani15152319","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T10:58:26Z","timestamp":1754564306000},"page":"2319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Salinity Stress on Antioxidant Enzyme Activity, Histopathology, and Gene Expression in the Hepatopancreas of the Oriental River Prawn, Macrobrachium nipponense"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6550-9268","authenticated-orcid":false,"given":"Shubo","family":"Jin","sequence":"first","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghao","family":"Ye","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtuo","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sufei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"ref_1","first-page":"138","article-title":"Inland brackish (alkaline-saline) water resources and fisheries utilization in China","volume":"31","author":"Liang","year":"2013","journal-title":"Chin. 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