{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:36:49Z","timestamp":1760060209278,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFD1300205-9","202405410411233"],"award-info":[{"award-number":["2021YFD1300205-9","202405410411233"]}]},{"name":"Hubei Jingruitianheng Biotechechnology Co., Ltd.","award":["2021YFD1300205-9","202405410411233"],"award-info":[{"award-number":["2021YFD1300205-9","202405410411233"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Fermented wheat protein (FWP) has a high protein content and is beneficial to intestinal health, making it an ideal alternative to fishmeal (FM). This study evaluated the effects of replacing FM with FWP on growth performance, nutrient digestibility, and gut health in weaned piglets. A total of 144 weaned piglets (28 days old) were randomly divided into three dietary treatments with 48 piglets per treatment for a duration of 28 days. The treatments included a control diet containing 3% FM (CON group), a diet with 1.5% FM and 1.5% FWP (50% FWP replacement), and a diet with 3% FWP (100% FWP replacement). The results showed that replacing FM with FWP did not significantly affect average daily gain, average daily feed intake, or the feed conversion ratio but significantly reduced diarrhea rates (p &lt; 0.01). The 50% and 100% FWP replacement significantly improved the digestibility of crude protein and acid detergent fiber, respectively (p &lt; 0.05). The 100% FWP replacement significantly improved the activity of trypsin and chymotrypsin in the duodenum and jejunum (p &lt; 0.05). Additionally, the 100% FWP replacement significantly enhanced serum total antioxidant capacity, glutathione peroxidase, and immunoglobulin A contents (p &lt; 0.05). The 100% FWP replacement significantly increased villus height in the jejunum (p &lt; 0.01). Moreover, the 100% FWP replacement significantly increased the expression of intestinal-barrier-related genes Zonula Occludens-1 and occludin (p &lt; 0.01) and decreased the expression of inflammation-related genes (p &lt; 0.01). Overall, FWP effectively reduces diarrhea rates, improves gut structure, enhances digestion, and boosts antioxidant and immune functions, particularly at a high replacement ratio (100%). Therefore, FWP can be considered an effective alternative to FM.<\/jats:p>","DOI":"10.3390\/ani15162362","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T07:53:44Z","timestamp":1754985224000},"page":"2362","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Replacing Fishmeal with Fermented Wheat Protein Improves Nutrient Digestibility and Intestinal Health in Weaned Piglets"],"prefix":"10.3390","volume":"15","author":[{"given":"Nuo","family":"Xiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaokang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Lin","sequence":"additional","affiliation":[{"name":"Hubei Jingruitianheng Biotechechnology Co., Ltd., Yichang 443000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanseng","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changhua","family":"Lai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.5713\/ab.22.0072","article-title":"Blend of essential oils can reduce diarrheal disorders and improve liver antioxidant status in weaning piglets","volume":"36","author":"Grando","year":"2023","journal-title":"Anim. Biosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.aninu.2017.12.004","article-title":"Gastrointestinal tract (gut) health in the young pig","volume":"4","author":"Pluske","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Modina, S.C., Polito, U., Rossi, R., Corino, C., and Di Giancamillo, A. (2019). Nutritional Regulation of Gut Barrier Integrity in Weaning Piglets. Animals, 9.","DOI":"10.3390\/ani9121045"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.livsci.2019.04.016","article-title":"Effects of replacing soybean meal, soy protein concentrate, fermented soybean meal or fish meal with enzyme-treated soybean meal on growth performance, nutrient digestibility, antioxidant capacity, immunity and intestinal morphology in weaned pigs","volume":"225","author":"Ma","year":"2019","journal-title":"Livest. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"skad087","DOI":"10.1093\/jas\/skad087","article-title":"Torula yeast may improve intestinal health and immune function of weanling pigs","volume":"101","author":"Espinosa","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_6","first-page":"574","article-title":"Effects of enzymatically hydrolyzed wheat gluten on growth performance, antioxidant status, and immune function in weaned pigs","volume":"97","author":"Han","year":"2017","journal-title":"Can. J. Anim. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Gabler, A.M., and Scherf, K.A. (2020). Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins. Biomolecules, 10.","DOI":"10.3390\/biom10091227"},{"key":"ref_8","first-page":"20220222602","article-title":"Wheat oligopeptides enhance the intestinal mucosal barrier and alleviate inflammation via theTLR4\/Myd88\/MAPK signaling pathway in aged mice","volume":"66","author":"Yang","year":"2022","journal-title":"Food Nutr. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lima, M.S., de Lima, V.C.O., Piuvezam, G., de Azevedo, K.P.M., Maciel, B.L.L., and Morais, A.H.D. (2022). Mechanisms of action of anti-inflammatory proteins and peptides with anti-TNF-alpha activity and their effects on the intestinal barrier: A systematic review. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0270749"},{"key":"ref_10","unstructured":"Van Krimpen, M.M., and Binnendijk, G.P. (2001). The Effect of Wheat-Protein in Diets on the Performance and Health of Weanling Piglets, Praktijkonderzoek Veehouderij. Number 233."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"768","DOI":"10.3724\/SP.J.1006.2016.00768","article-title":"Contents of Protein and Amino Acids of Wheat Grain in Different Wheat Production Regions and Their Evaluation","volume":"42","author":"Liu","year":"2016","journal-title":"Acta Agron. Sin."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e02345-22","DOI":"10.1128\/spectrum.02345-22","article-title":"Dietary supplementation with Lactiplantibacillus plantarum P-8 improves the growth performance and gut microbiota of weaned piglets","volume":"12","author":"Yu","year":"2024","journal-title":"Microbiol. Spectrum"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"517","DOI":"10.22358\/jafs\/189648\/2024","article-title":"Effect of dietary wheat gluten levels on intestinal mucin flow and composition in young pigs","volume":"33","author":"Swiech","year":"2024","journal-title":"J. Anim. Feed Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Jia, N., Jin, J., Wei, X., Trabalza-Marinucci, M., Jia, G., Zhou, Q., Zhang, R., Li, H., Wang, F., and Zhao, H. (2025). Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1561196"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1080\/00071668.2017.1315051","article-title":"Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens","volume":"58","author":"Jazi","year":"2017","journal-title":"Br. Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"101615","DOI":"10.1016\/j.psj.2021.101615","article-title":"Dietary hydrolyzed wheat gluten supplementation ameliorated intestinal barrier dysfunctions of broilers challenged with Escherichia coli O78","volume":"101","author":"Wu","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"skae229","DOI":"10.1093\/jas\/skae229","article-title":"Effects of wheat-based fermented liquid feed on growth performance, nutrient digestibility, gut microbiota, intestinal morphology, and barrier function in grower-finisher pigs","volume":"102","author":"Zhang","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_18","first-page":"409","article-title":"Effects of dietary supplementation of fermented wheat bran on performance and blood profiles in weaned pigs","volume":"44","author":"Jeong","year":"2017","journal-title":"Korean J. Agric. Sci."},{"key":"ref_19","first-page":"150","article-title":"Effects of fermented wheat bran on growth performance, nutrient apparent digestibility, immune function and fecal microbiota of weaned piglets","volume":"34","author":"Fang","year":"2022","journal-title":"Chin. J. Anim. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Benzie, I.F.F., and Strain, J.J. (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of \u201cAntioxidant Power\u201d: The FRAP Assay, Elsevier.","DOI":"10.1006\/abio.1996.0292"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1079\/BJN19960062","article-title":"Apparent ileal dry matter and crude protein digestibility of rations fed to pigs and determined with the use of chromic oxide (Cr2O3) and acid-insoluble ash as digestive markers","volume":"76","author":"Veldman","year":"1996","journal-title":"Br. J. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"917","DOI":"10.5713\/ajas.2014.r.02","article-title":"Evaluation of Amino Acid and Energy Utilization in Feedstuff for Swine and Poultry Diets","volume":"27","author":"Kong","year":"2014","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chia, S.Y., Tanga, C.M., Osuga, I.M., Alaru, A.O., Mwangi, D.M., Githinji, M., Subramanian, S., Fiaboe, K.K.M., Ekesi, S., and vanLoon, J.J.A. (2019). Effect of Dietary Replacement of Fishmeal by Insect Meal on Growth Performance, Blood Profiles and Economics of Growing Pigs in Kenya. Animals, 9.","DOI":"10.3390\/ani9100705"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"skae293","DOI":"10.1093\/jas\/skae293","article-title":"A blend of bacillus-fermented soybean meal, functional amino acids, and nucleotides improves nutrient digestibility, bolsters immune response, reduces diarrhea, and enhances growth performance in weaned piglets","volume":"102","author":"Tran","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Upadhaya, S.D., and Kim, I.H. (2021). The Impact of Weaning Stress on Gut Health and the Mechanistic Aspects of Several Feed Additives Contributing to Improved Gut Health Function in Weanling Piglets\u2014A Review. Animals, 11.","DOI":"10.3390\/ani11082418"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Qiu, Y., Tang, J., Wang, L., Yang, X., and Jiang, Z. (2024). Fermented Corn-Soybean Meal Improved Growth Performance and Reduced Diarrhea Incidence by Modulating Intestinal Barrier Function and Gut Microbiota in Weaned Piglets. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25063199"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Muniyappan, M., Shanmugam, S., Park, J.H., Han, K., and Kim, I.H. (2023). Effects of fermented soybean meal supplementation on the growth performance and apparent total tract digestibility by modulating the gut microbiome of weaned piglets. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-30698-6"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Czech, A., Grela, E.R., and Kiesz, M. (2021). Dietary fermented rapeseed or\/and soybean meal additives on performance and intestinal health of piglets. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-96117-w"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"399","DOI":"10.5713\/ajas.2007.399","article-title":"Effect of feeding levels of microbial fermented soy protein on the growth performance, nutrient digestibility and intestinal morphology in weaned piglets","volume":"20","author":"Kim","year":"2007","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110579","DOI":"10.1016\/j.fct.2019.110579","article-title":"Hydrolyzed wheat gluten alleviates deoxynivalenol-induced intestinal injury by promoting intestinal stem cell proliferation and differentiation via upregulation of Wn\/\u03b2-catenin signaling in mice","volume":"131","author":"Zhou","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yu, L., Li, H., Peng, Z., Ge, Y., Liu, J., Wang, T., Wang, H., and Dong, L. (2021). Early Weaning Affects Liver Antioxidant Function in Piglets. Animals, 11.","DOI":"10.3390\/ani11092679"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6262","DOI":"10.1002\/jsfa.13456","article-title":"Dietary rutin improves the antidiarrheal capacity of weaned piglets by improving intestinal barrier function, antioxidant capacity and cecal microbiota composition","volume":"104","author":"Ma","year":"2024","journal-title":"J. Sci. Food Agric."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liu, H., Hu, J., Mahfuz, S., and Piao, X. (2020). Effects of Hydrolysable Tannins as Zinc Oxide Substitutes on Antioxidant Status, Immune Function, Intestinal Morphology, and Digestive Enzyme Activities in Weaned Piglets. Animals, 10.","DOI":"10.3390\/ani10050757"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wlazlo, L., Nowakowicz-Debek, B., Ossowski, M., Lukaszewicz, M., and Czech, A. (2022). Effect of Fermented Rapeseed Meal in Diets for Piglets on Blood Biochemical Parameters and the Microbial Composition of the Feed and Faeces. Animals, 12.","DOI":"10.3390\/ani12212972"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zhao, P., Hou, Y., Wang, Z., Liao, A., Pan, L., Zhang, J., Dong, Y., Hu, Z., Huang, J., and Ou, X. (2023). Effect of fermentation on structural properties and antioxidant activity of wheat gluten by Bacillus subtilis. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1116982"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3136\/fstr.8.227","article-title":"Isolation and characterization of peptides with antioxidant activity derived from wheat gluten","volume":"8","author":"Suetsuna","year":"2002","journal-title":"Food Sci. Technol. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1080\/09540105.2015.1007448","article-title":"Effects of antibacterial peptide on humoral immunity in weaned piglets","volume":"26","author":"Yuan","year":"2015","journal-title":"Food Agric. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, M., Yang, Z., Xu, F., Zhang, J., Luo, X., Ma, Y., Pang, H., Duan, Y., Chen, J., and Cai, Y. (2024). Effects of Probiotic-Fermented Feed on the Growth Profile, Immune Functions, and Intestinal Microbiota of Bamei Piglets. Animals, 14.","DOI":"10.3390\/ani14040647"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, S., Xiao, H., Xiong, Y., Chen, J., Wu, Q., Wen, X., Jiang, Z., and Wang, L. (2022). Effects of Fermented Feed on the Growth Performance, Intestinal Function, and Microbiota of Piglets Weaned at Different Age. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.841762"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"110934","DOI":"10.1016\/j.vetimm.2025.110934","article-title":"Effects of Saccharomyces cerevisiae fermentation-derived postbiotics supplementation in sows and piglets\u2019 diet on intestinal morphology, and intestinal barrier function in weaned pigs in an intensive pig production system","volume":"283","author":"Hung","year":"2025","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_41","first-page":"463","article-title":"Effect of fermented liquid feeds on production parameters and gut microbiota composition of weaned piglets and growing pigs","volume":"144","author":"Varga","year":"2022","journal-title":"Magy. Allatorv. Lapja"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1590\/S1516-89132005000800008","article-title":"Effect of probiotic and prebiotic inclusion in weaned piglet diets on structure and ultra-structure of small intestine","volume":"48","author":"Thomaz","year":"2005","journal-title":"Braz. Arch. Biol. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"de Groot, N., Fari\u00f1as, F., Cabrera-G\u00f3mez, C.G., Pallares, F.J., and Ramis, G. (2024). Blend of organic acids improves gut morphology and affects inflammation response in piglets after weaning. Front. Anim. Sci., 5.","DOI":"10.3389\/fanim.2024.1308514"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/16\/2362\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:25:13Z","timestamp":1760034313000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/16\/2362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,12]]},"references-count":43,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["ani15162362"],"URL":"https:\/\/doi.org\/10.3390\/ani15162362","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,8,12]]}}}