{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:20:31Z","timestamp":1782858031666,"version":"3.54.5"},"reference-count":81,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Artificial insemination (AI) in rabbits depends largely on chilled semen storage, but the physiological responses to chilling and associated biochemical changes in seminal plasma (SP) remain poorly understood, particularly across breeds. This study aimed to compare the semen preservation capacity of Algerian local population (LAP) and New Zealand White (NZW) rabbits and to explore the relationship between SP oxidative stress biomarkers and sperm traits during 72 h of chilled storage at 5 \u00b0C. Semen pools (nine\/breed) were evaluated at 0, 4, 24, 48, and 72 h for motility, viability, and acrosome status. Oxidative stress markers were also assessed in the SP, including malondialdehyde (MDA), reactive oxygen metabolites (ROMs), superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT). LAP sperm showed higher motility (p &lt; 0.001) and viability (p &lt; 0.05), particularly between 4 h and 48 h, and exhibited a lower rate of acrosome reaction (p &lt; 0.001) from 48 h to 72 h. Lower SOD and higher CAT activity in LAP (p &lt; 0.001), correlated with MDA and acrosome status, respectively, may reflect a more balanced antioxidant response. Lipid peroxidation did not appear to be the main factor driving sperm deterioration (p &gt; 0.05). These results demonstrate that LAP rabbits exhibit better resilience to chilled storage compared to NZW and highlight the potential value of CAT and SOD activities as indicators of sperm resilience during chilled storage. Further studies are required to validate and extend these findings, with the aim of improving semen preservation strategies.<\/jats:p>","DOI":"10.3390\/ani15162384","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T14:51:46Z","timestamp":1755183106000},"page":"2384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Breed-Specific Responses of Rabbit Semen to Chilling Storage: Sperm Quality, Acrosome Status, and Oxidative Stress Biomarkers"],"prefix":"10.3390","volume":"15","author":[{"given":"Ibtissem","family":"Boulbina","sequence":"first","affiliation":[{"name":"Laboratory of Research \u201cHealth and Animal Productions\u201d, Higher National Veterinary School \u201cRabie Bouchama\u201d, Issad Abbes Street, Oued Smar, Algiers 16000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed El Amine","family":"Bekara","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hacina","family":"AinBaziz","sequence":"additional","affiliation":[{"name":"Laboratory of Research \u201cHealth and Animal Productions\u201d, Higher National Veterinary School \u201cRabie Bouchama\u201d, Issad Abbes Street, Oued Smar, Algiers 16000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5063-6785","authenticated-orcid":false,"given":"Simona","family":"Mattioli","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Environmental Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6134-0901","authenticated-orcid":false,"given":"Cesare","family":"Castellini","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Environmental Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.5455\/vetworld.2012.301-310","article-title":"Assisted Reproductive Techniques in Farm Animal\u2014From Artificial Insemination to Nanobiotechnology","volume":"5","author":"Verma","year":"2012","journal-title":"Vet. 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