{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T06:47:30Z","timestamp":1785048450955,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Mild\u2013moderate equine asthma (MEA) is a very common but underdiagnosed pulmonary disease in horses, with mild cases not showing clinical respiratory signs. This study evaluates the influence of a standardized lunging exercise test (SLET) on bronchoalveolar lavage fluid (BALF) cytology in MEA horses. We hypothesized that SLET would increase the total nucleated cell count (TNCC) and\/or percentages of inflammatory cells associated with EA. In a prospective, randomized, non-blinded, between-subjects study design of two independent groups, 39 horses (17 mild and 22 moderate) were included. They were chosen out of a cohort of horses undergoing respiratory investigations (history, clinical examination, and clinical pathology (white blood cells (WBC) and arterial blood gas analysis (aBGA)) consistent with MEA, using a scoring system in a clinical setting of an equine referral clinic. Bronchoalveolar lavage (BAL) was performed 30 min post-SLET in 16 randomly chosen horses. The other horses underwent BAL without SLET. The SLET resulted in a statistically significant increase (p &lt; 0.001) in the proportions of neutrophils in BALF cytology, and in an increased chance of confirmation of the presumed diagnosis in horses with mild phenotypes (p &lt; 0.001, OR = 8.00, CI = 1.28\u201350.04), while moderate phenotypes were less frequently diagnosed. Exercise had no association with cytology across all horses. Unexpectedly, the SLET group of horses with a moderate phenotype had a statistically significant lower TNCC (p = 0.035). In conclusion, an SLET prior to BAL might increase the chance of an MEA diagnosis.<\/jats:p>","DOI":"10.3390\/ani15162428","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T10:59:01Z","timestamp":1755601141000},"page":"2428","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Influence of a Standardized Lunging Exercise Test on BALF Cytology in Horses Suffering from Mild\u2013Moderate Equine Asthma"],"prefix":"10.3390","volume":"15","author":[{"given":"Lioba","family":"Lendl","sequence":"first","affiliation":[{"name":"Equine Clinic Hochmoor, 48712 Gescher, Germany"},{"name":"School of Veterinary Medicine, Freie Universitaet Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Wirth","sequence":"additional","affiliation":[{"name":"Equine Clinic Hochmoor, 48712 Gescher, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-2926","authenticated-orcid":false,"given":"Roswitha","family":"Merle","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Epidemiology and Biostatistics, School of Veterinary Medicine, Freie Universitaet Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3333-9647","authenticated-orcid":false,"given":"Ann Kristin","family":"Barton","sequence":"additional","affiliation":[{"name":"Equine Clinic Hochmoor, 48712 Gescher, Germany"},{"name":"School of Veterinary Medicine, Freie Universitaet Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/jvim.13824","article-title":"Inflammatory Airway Disease of Horses\u2014Revised Consensus Statement","volume":"30","author":"Cardwell","year":"2016","journal-title":"J. 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