{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T22:02:03Z","timestamp":1789250523697,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T00:00:00Z","timestamp":1755907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Special project of the Third Xinjiang Comprehensive Scientific Expedition of the Ministry of Science and Technology","award":["2022xjkk150403"],"award-info":[{"award-number":["2022xjkk150403"]}]},{"name":"Special project of the Third Xinjiang Comprehensive Scientific Expedition of the Ministry of Science and Technology","award":["2023YFD2401004"],"award-info":[{"award-number":["2023YFD2401004"]}]},{"name":"Sub-project of the National Key Research and Development Program","award":["2022xjkk150403"],"award-info":[{"award-number":["2022xjkk150403"]}]},{"name":"Sub-project of the National Key Research and Development Program","award":["2023YFD2401004"],"award-info":[{"award-number":["2023YFD2401004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>To systematically elucidate the chronological patterns of embryonic development and morphological changes in the larval and juvenile stages of Silurus glanis, and provide fundamental biological insights into this species, in this study, fertilized eggs were obtained through artificial spawning induction technology. After removing adhesiveness from fertilized eggs using trypsin, a detailed developmental study was conducted. The study systematically analyzed the chronological sequence of embryonic development and the morphological change patterns of larval and juvenile fish. The results showed the following: The fertilized eggs of S. glanis are yellow, spherical, and sticky, and the stickiness allows eggs to attach to spawning substrates, enhancing hatching success. The egg diameter after water absorption was (2.88 \u00b1 0.13) mm. The embryonic development took 47 h and 55 min, with a total accumulated temperature of 1245.56 h degrees Celsius, the developmental process includes seven stages and twenty-six periods, namely the zygophase stage, cleavage stage, blastula stage, gastrula stage, neurula stage, organogenesis stage, and hatching stage. At a temperature of (26.0 \u00b1 0.9) \u00b0C, the hatched individuals went through the pre-yolk sac larval stage, late larval stage, juvenile fry stage, and juvenile stage. In the pre-yolk sac larval stage, otoliths appeared in the bilateral otic vesicles, a pair of barbel primordia emerged under the mandible, a short and thin straight intestine formed in the abdominal cavity, and the oral fissure first appeared. In the late larval stage, the fin rays were initially formed, the intestine became thicker and longer, the oral fissure, anus, and cloaca were formed, and the larvae could float and start feeding on exogenous food. In the juvenile fry stage, the differentiation of various organs was basically complete, the nostrils became larger, and both the anal fin and caudal fin had dark black markings. In the juvenile stage, the maxillary barbels elongated, the mucus layer thickened on the body and back, the abdomen is light white, and it had the external morphological characteristics of an adult fish. By measuring and calculating the total length, body length, body height, and head length of S. glanis larvae and juveniles (0\u201340 days), the results showed that the growth characteristics conformed to the following fish growth formula: TL = 0.0141x2 + 0.8096x + 8.2421 (R2 = 0.9916), where x denotes days after hatching. This study has preliminarily mastered the chronological patterns of the embryonic development, growth, and formation of the morphological characteristics in larval and juvenile S. glanis, providing scientific data and laying a theoretical foundation for the division of early developmental stages, reproduction, hatching, and fry cultivation.<\/jats:p>","DOI":"10.3390\/ani15172478","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:09:32Z","timestamp":1756080572000},"page":"2478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Study on the Morphological Development Timeline and Growth Model of Embryos and Larvae of European Catfish (Silurus glanis)"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhuoleaersi","family":"Adakebaike","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Tarim University, Alar 843300, China"},{"name":"Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5665-2854","authenticated-orcid":false,"given":"Zhengwei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Tarim University, Alar 843300, China"},{"name":"Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hudelati","family":"Anasi","sequence":"additional","affiliation":[{"name":"Xinjiang Aquatic Science Research Institute\/Northwest Fisheries Resources and Environment Scientific Observation and Experiment Station of the Ministry of Agriculture, Urumqi 830000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangtao","family":"He","sequence":"additional","affiliation":[{"name":"Xinjiang Aquatic Science Research Institute\/Northwest Fisheries Resources and Environment Scientific Observation and Experiment Station of the Ministry of Agriculture, Urumqi 830000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejie","family":"Zhai","sequence":"additional","affiliation":[{"name":"Xinjiang Aquatic Science Research Institute\/Northwest Fisheries Resources and Environment Scientific Observation and Experiment Station of the Ministry of Agriculture, Urumqi 830000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunming","family":"Shi","sequence":"additional","affiliation":[{"name":"Xinjiang Aquatic Science Research Institute\/Northwest Fisheries Resources and Environment Scientific Observation and Experiment Station of the Ministry of Agriculture, Urumqi 830000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhulan","family":"Nie","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Tarim University, Alar 843300, China"},{"name":"Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mahalder, B., Haque, M.M., Siddique, M.A.B., Hasan, N.A., Alam, M.M., Talukdar, M.M.N., Shohan, M.H., Ahasan, N., Hasan, M.M., and Ahammad, A.K.S. (2023). Embryonic and Larval Development of Stinging Catfish, Heteropneustes fossilis, in Relation to Climatic and Water Quality Parameters. Life, 13.","DOI":"10.3390\/life13020583"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/jfb.12432","article-title":"Early environment influences later performance in fishes","volume":"85","author":"Jonsson","year":"2014","journal-title":"J. Fish Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/dvdy.388","article-title":"Stages of embryonic development in the live-bearing fish, Gambusia holbrooki","volume":"251","author":"Mousavi","year":"2022","journal-title":"Dev. Dyn."},{"key":"ref_4","first-page":"1370","article-title":"Temperature during embryonic development has persistent effects on metabolic enzymes in the muscle of zebrafish","volume":"217","author":"Schnurr","year":"2014","journal-title":"J. Exp. Biol."},{"key":"ref_5","unstructured":"Zhu, S. (1995). Synopsis of Freshwater Fishes of China, Jiangsu Science and Technology Press."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Guo, Y. (2012). Xinjiang Fisheries Records, Xinjiang Science and Technology Press.","DOI":"10.1057\/9781137287670_30"},{"key":"ref_7","first-page":"961","article-title":"Experimental Study of Capruring and Short Distance Transportation with in Situ Temporary Rising of Silurus glanis Linnaeus In Yili Rever","volume":"52","author":"Keerjiang","year":"2015","journal-title":"Xinjiang Agric. Sci."},{"key":"ref_8","unstructured":"Ren, M., Guo, Y., Zhang, Q., Zhang, R., Li, H., Keerjiang, A., Cai, L., Yong, W., Ren, B., and Gao, H. (1998). Fisheries Resources and Fishery of River Yili, Heilongjiang Science and Technology Press."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1111\/faf.12774","article-title":"Life in the fast lane: Revisiting the fast growth\u2014High survival paradigm during the early life stages of fishes","volume":"24","author":"Robert","year":"2023","journal-title":"Fish Fish."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1111\/jfb.14428","article-title":"Influence of light intensity and photoperiod on embryonic development, survival and growth of threatened catfish Ompok bimaculatus early larvae","volume":"97","author":"Arambam","year":"2020","journal-title":"J. Fish Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Harada, A.E., Lindgren, E.A., Hermsmeier, M.C., Rogowski, P.A., Terrill, E., and Burton, R.S. (2015). Monitoring Spawning Activity in a Southern California Marine Protected Area Using Molecular Identification of Fish Eggs. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0134647"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3463","DOI":"10.1002\/ece3.6144","article-title":"Efficacy of metabarcoding for identification of fish eggs evaluated with mock communities","volume":"10","author":"Duke","year":"2020","journal-title":"Ecol. Evol."},{"key":"ref_13","first-page":"303","article-title":"Observation of embryonic and larval development of sea bass Dicentrarchus labrax","volume":"34","author":"Ma","year":"2019","journal-title":"J. Dalian Ocean. Univ."},{"key":"ref_14","first-page":"1285","article-title":"Artificial Spawning and Feeding of European Catfish, Siluris glanis L., in Turkey","volume":"7","author":"Kenan","year":"2008","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"142","DOI":"10.14798\/73.4.846","article-title":"Effects of feeding regime on growth feed conversion and size variation of Silurus glanis","volume":"73","author":"Havas","year":"2015","journal-title":"Croat. J. Fish."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"385","DOI":"10.4194\/1303-2712-v15_2_23","article-title":"Comparison of the results from induced breeding of European catfish (Silurus glanis L.) broodstock reared in an intensive system or in pond conditions","volume":"15","author":"Szabo","year":"2015","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_17","first-page":"25","article-title":"Danube Six Silurus glanis Linnaeus Biology and its Culturing Observation","volume":"3","author":"Wang","year":"1993","journal-title":"Freshw. Fish."},{"key":"ref_18","first-page":"1335","article-title":"Studies on the Tolerance of Silurus glaris Linnaeus Fingerlings to Salinity and Alkalinity","volume":"56","author":"Wei","year":"2019","journal-title":"Xinjiang Agric. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Barbacariu, C.A., Rimbu, C.M., Burducea, M., Dirvariu, L., Miron, L.D., Boiangiu, R.S., Dumitru, G., and Todirascu-Ciornea, E. (2023). Comparative Study of Flesh Quality, Blood Profile, Antioxidant Status, and Intestinal Microbiota of European Catfish (Silurus glanis) Cultivated in a Recirculating Aquaculture System (RAS) and Earthen Pond System. Life, 13.","DOI":"10.3390\/life13061282"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Simeanu, C., M\u0103gdici, E., P\u0103s\u0103rin, B., Avarvarei, B.-V., and Simeanu, D. (2022). Quantitative and Qualitative Assessment of European Catfish (Silurus glanis) Flesh. Agriculture, 12.","DOI":"10.3390\/agriculture12122144"},{"key":"ref_21","first-page":"174","article-title":"Artificial Propagation and Seedling Rearing Technology of Silurus soidatovi","volume":"40","author":"Zeng","year":"2013","journal-title":"Fish. Sci. Technol. Inf."},{"key":"ref_22","first-page":"602","article-title":"Ultrastructure of spermatozoon and effects of pH and temperature onspermatozoon motility in oriental sheatfish Silurus asotus","volume":"31","author":"Qiao","year":"2016","journal-title":"J. Dalian Ocean. Univ."},{"key":"ref_23","first-page":"584","article-title":"Embryonic development and growth of larval and juvenile basa fish striped catfish (Pangasianodon hypophthalmus) and the determination of growth model","volume":"38","author":"Ni","year":"2023","journal-title":"J. Dalian Ocean. Univ."},{"key":"ref_24","first-page":"9","article-title":"Observation and Study on Embryos and Postembryonic Development of Pseudobagrus fulvidraco","volume":"5","author":"Wang","year":"1989","journal-title":"Freshw. Fish."},{"key":"ref_25","first-page":"9","article-title":"A Study of the embryonic development of Silurus asotus Linnaeus and the effect of water temperature on it","volume":"3","author":"Xiao","year":"1998","journal-title":"J. South China Norm. Univ. (Nat. Sci.)"},{"key":"ref_26","first-page":"662","article-title":"Artificial reproduction and embryonic development of Onychostoma rara","volume":"31","author":"Han","year":"2024","journal-title":"J. Fish. Sci. China"},{"key":"ref_27","first-page":"569","article-title":"Artificial spawning and embryonic development of freshwater drum, Aplodinotus grunniens","volume":"28","author":"Zhang","year":"2021","journal-title":"J. Fish. Sci. China"},{"key":"ref_28","first-page":"333","article-title":"Study on the early embryonic development of Claris lareza","volume":"23","author":"Lin","year":"1999","journal-title":"J. Nanchang Univ. (Nat. Sci.)"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1086\/BBLv216n3p226","article-title":"Egg size as a life history character of marine invertebrates: Is it all it\u2019s cracked up to be?","volume":"216","author":"Moran","year":"2009","journal-title":"Biol. Bull."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/BF00323776","article-title":"Interactions among stream fishes: Predator-induced habitat shifts and larval survival","volume":"87","author":"Harvey","year":"1991","journal-title":"Oecologia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"105200","DOI":"10.1016\/j.prevetmed.2020.105200","article-title":"Comparative assessment of live cyprinid and salmonid movement networks in England and Wales","volume":"185","author":"Tidbury","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.bpj.2020.06.036","article-title":"Mechanical Characteristics of Ultrafast Zebrafish Larval Swimming Muscles","volume":"119","author":"Mead","year":"2020","journal-title":"Biophys. J."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Vea, I.M., and Shingleton, A.W. (2021). Network-regulated organ allometry: The developmental regulation of morphological scaling. Wiley Interdiscip. Rev. Dev. Biol., 10.","DOI":"10.1002\/wdev.391"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"101159","DOI":"10.1016\/j.aqrep.2022.101159","article-title":"Transition from endogenous to exogenous feeding in hatchery-cultured European eel larvae","volume":"24","author":"Benini","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_35","first-page":"844","article-title":"Ontogenetic allometry in larval and Juvenile clown anemonefIsh Amphiprion ocellaris","volume":"44","author":"Hu","year":"2020","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_36","first-page":"1207","article-title":"Embryological Stages and Allometric Growth During Yolk-Sac Larvae of Lampetra japonica (Martens)","volume":"41","author":"Li","year":"2017","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00114-003-0424-5","article-title":"Development of catfish lateral line organs: Electroreceptors require innervation, although mechanoreceptors do not","volume":"90","author":"Roth","year":"2003","journal-title":"Naturwissenschaften"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1002\/(SICI)1096-9861(20000612)421:4<570::AID-CNE7>3.0.CO;2-6","article-title":"Distribution and innervation of lateral line organs in the channel catfish","volume":"421","author":"Northcutt","year":"2000","journal-title":"J. Comp. Neurol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1111\/j.1095-8649.2008.02038.x","article-title":"Development of sensory organ in larvae of African catfish Clarias gariepinus","volume":"73","author":"Mukai","year":"2008","journal-title":"J. Fish Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4434","DOI":"10.1111\/are.15282","article-title":"Determination of a predictive growth model for cultivated African catfish Clarias gariepinus (Burchell, 1882)","volume":"52","author":"Musa","year":"2021","journal-title":"Aquac. Res."},{"key":"ref_41","first-page":"16","article-title":"Production of the African catfish (Clarias gariepinus) I. Growth, mortality and yield south of the Zambezi","volume":"10","author":"Clay","year":"1984","journal-title":"J. Limnol. Soc. S. Afr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1439-0426.1985.tb00407.x","article-title":"The influence of temperature on the growth of the European catfish (Silurus glanis L.)","volume":"1","author":"Hilge","year":"2007","journal-title":"J. Appl. Ichthyol."},{"key":"ref_43","first-page":"1379","article-title":"Daphnia\u2019s challenge: Survival and reproduction when calcium and food are limiting","volume":"38","author":"Goodberry","year":"2016","journal-title":"J. Plankton Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1439-0264.2007.00812.x","article-title":"The development of the digestive tract in larval European catfish (Silurus glanis L.)","volume":"37","author":"Kuzir","year":"2008","journal-title":"Anat. Histol. Embryol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1111\/jfb.15498","article-title":"First insights into the activity of major digestive enzymes in the intestine of the European catfish Silurus glanis and protective anti-enzymatic potential of its gut parasite Silurotaenia siluri","volume":"103","author":"Frolova","year":"2023","journal-title":"J. Fish Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0165-7836(91)90104-N","article-title":"A comparison of non-linear growth models for fisheries","volume":"11","author":"Pawlak","year":"1991","journal-title":"Fish. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s11160-010-9168-4","article-title":"Age and growth of the European catfish (Silurus glanis) in a Turkish Reservoir and comparison with introduced populations","volume":"21","author":"Alp","year":"2011","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3750\/AIP2014.44.3.03","article-title":"Growth, cannibalism, and survival relations in larvae of European catfish, Silurus glanis (Actinopterygii: Siluriformes: Siluridae)\u2014Attempts to mitigate sibling cannibalism","volume":"44","author":"Flisiak","year":"2014","journal-title":"Acta Ichthyol. Piscat."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/17\/2478\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:34:37Z","timestamp":1760034877000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/17\/2478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,23]]},"references-count":48,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["ani15172478"],"URL":"https:\/\/doi.org\/10.3390\/ani15172478","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,23]]}}}