{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:23:56Z","timestamp":1791048236403,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Japan Society for the Promotion of Science (JSPS)","award":["JP21KK0124"],"award-info":[{"award-number":["JP21KK0124"]}]},{"name":"the Japan Society for the Promotion of Science (JSPS)","award":["JP22K19896"],"award-info":[{"award-number":["JP22K19896"]}]},{"name":"the Japan Society for the Promotion of Science (JSPS)","award":["JP23K23764"],"award-info":[{"award-number":["JP23K23764"]}]},{"name":"the Japan Society for the Promotion of Science (JSPS)","award":["JP23K23805"],"award-info":[{"award-number":["JP23K23805"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Mitochondrial-targeted antioxidant supplementation is promising for in vitro culture of mammalian embryos. Elamipretide (SS-31) is a synthetic tetrapeptide that binds to the inner mitochondrial membrane, contributing to the prevention of oxidative stress. In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated. Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 \u00b5M, with 0 \u00b5M and DMSO-treated groups established as controls. In vitro fertilization and embryo culture were performed to analyze developmental potential. Oocytes cultured in medium with 1 \u00b5M SS-31 exhibited higher maturation and blastocyst formation rates than the control (0 \u00b5M) (78.3 \u00b1 3.8% vs. 55.2 \u00b1 4.1% and 7.6 \u00b1 1.6% vs. 2.8 \u00b1 1.8%, respectively). Oocytes treated with 1 \u00b5M SS-31 showed significantly lower reactive oxygen species, higher glutathione content, and improved mitochondrial membrane potential than those without treatment. DNA fragmentation in oocytes after in vitro maturation was significantly lower in the 1 \u00b5M SS-31 supplemented group than in the control. This study demonstrates that SS-31 exerts beneficial effects on the in vitro production of porcine embryos via the enhancement of the mitochondrial antioxidant capacity.<\/jats:p>","DOI":"10.3390\/ani15172497","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:25:57Z","timestamp":1756189557000},"page":"2497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mitochondrial-Targeted Protective Potential of Elamipretide for the In Vitro Production of Porcine Embryos"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7325-4207","authenticated-orcid":false,"given":"Suong T.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0382-959X","authenticated-orcid":false,"given":"Takeshige","family":"Otoi","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"},{"name":"Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia"},{"name":"Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Namula","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0073-1394","authenticated-orcid":false,"given":"Oky Setyo","family":"Widodo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0225-5854","authenticated-orcid":false,"given":"Theerawat","family":"Tharasanit","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9470-8426","authenticated-orcid":false,"given":"Kaywalee","family":"Chatdarong","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7045-3922","authenticated-orcid":false,"given":"Megumi","family":"Nagahara","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"},{"name":"Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Nakai","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4673-1581","authenticated-orcid":false,"given":"Maki","family":"Hirata","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"},{"name":"Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuminori","family":"Tanihara","sequence":"additional","affiliation":[{"name":"Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan"},{"name":"Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41392-023-01547-9","article-title":"Mitochondrial dynamics in health and disease: Mechanisms and potential targets","volume":"8","author":"Chen","year":"2023","journal-title":"Signal Transduct. 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