{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:08:37Z","timestamp":1793106517405,"version":"4.5.0"},"reference-count":59,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shandong Key Science and Technology Innovation Program","award":["2019JZZY010704"],"award-info":[{"award-number":["2019JZZY010704"]}]},{"name":"Agricultural Science and Technology Innovation Program (ASTIP) of the Chinese Academy of Agricultural Sciences","award":["2019JZZY010704"],"award-info":[{"award-number":["2019JZZY010704"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In recent years, scientific interest in functional ingredients capable of replacing the non-therapeutic use of antibiotics in animal feed has intensified, fostering the exploration of novel additives such as Saccharomyces cerevisiae hydrolysate (SCH). This study investigated the effect of dietary SCH supplementation on growth performance, intestinal morphology, local immune response, and cecal microbiota composition in Ross 308 broiler chickens. A total of 300 one-day-old male chicks were randomly assigned to two experimental groups, receiving either a standard diet or a diet supplemented with SCH (500 mg\/kg during the starter and grower phases; 250 mg\/kg during the finisher phase). SCH supplementation significantly improved growth performance during the finisher phase, with increases in final body weight (p = 0.025), average daily gain (p = 0.049), and average daily feed intake (p = 0.027), without significant changes in feed conversion ratio (p &gt; 0.05). Favourable intestinal morphological modifications were observed, with a significant increase in villus height to crypt depth ratio in both the jejunum and ileum at days 28 (p = 0.035 and 0.002, respectively) and 42 (p &lt; 0.001). The expression of pro-inflammatory cytokine genes was significantly reduced, with lower levels of TNF-\u03b1, IL-1\u03b2, and IL-6, while tight junction protein genes ZO-1 and Occludin were significantly upregulated (p &lt; 0.05). Microbiota profiling revealed higher alpha diversity and greater abundance of Prevotella. These findings highlight SCH as a promising dietary strategy to improve broiler performance, intestinal function, and sustainability in poultry production systems.<\/jats:p>","DOI":"10.3390\/ani15172531","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:41:12Z","timestamp":1756381272000},"page":"2531","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of Saccharomyces cerevisiae Hydrolysate on Broiler Performance and Gut Health"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2303-170X","authenticated-orcid":false,"given":"Marcello","family":"Comi","sequence":"first","affiliation":[{"name":"Department of Human Science and Quality of Life Promotion, Universit\u00e0 Telematica San Raffaele, 00166 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8233-659X","authenticated-orcid":false,"given":"Davide","family":"Lanzoni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, 26900 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9477-2607","authenticated-orcid":false,"given":"Vera","family":"Perricone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, 26900 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-Ren","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1038\/s41545-020-0051-0","article-title":"Antibiotic Resistance Genes from Livestock Waste: Occurrence, Dissemination, and Treatment","volume":"3","author":"He","year":"2020","journal-title":"NPJ Clean Water"},{"key":"ref_2","first-page":"65","article-title":"Alternative Feed Additives to Antibiotics in Improving Health and Performance in Poultry and for the Prevention of Antimicrobials: A Review","volume":"6","author":"Okey","year":"2023","journal-title":"Niger. 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