{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:42:27Z","timestamp":1760060547498,"version":"build-2065373602"},"reference-count":65,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["n\u00ba 2019\/06940-1","20\u201322517J"],"award-info":[{"award-number":["n\u00ba 2019\/06940-1","20\u201322517J"]}]},{"name":"Czech Science Foundation","award":["n\u00ba 2019\/06940-1","20\u201322517J"],"award-info":[{"award-number":["n\u00ba 2019\/06940-1","20\u201322517J"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Chromosomal instability plays a significant role in karyotype evolution and speciation in mammalian groups with notable intraspecific chromosomal variation. The Cervidae family, known for its rapid karyotypic evolution due to chromosomal fragility, shows substantial chromosomal diversity, making it a focal point for studies on chromosomal evolution, particularly with respect to conservation and taxonomic classification. The Amazon gray brocket deer (Passalites nemorivagus) exhibits pronounced chromosomal polymorphism, including two distinct sex chromosome systems: the ancestral XX\/XY system and a new system due to an X\u2013autosome fusion (neo-X), where males present XY1Y2. This variation is intriguing, especially given that the effects on hybrids have not been previously reported. This study uses bovine whole-chromosome painting (WCP) and BAC probes to document karyotypic variation in P. nemorivagus. A male with the XY system and a heterozygous autosomal Robertsonian fusion was paired with a female with neo-X chromosomes, and the resulting female offspring displayed an X\u2013autosome fusion in heterozygosity. The females in this study, hybrids for the sex system, exhibited estrus, copulated, and both gave birth to offspring. This characterization is the first step in investigating the effects of sex chromosome system variation on hybrid viability and fertility, and provides insights into the reproductive biology of Neotropical deer.<\/jats:p>","DOI":"10.3390\/ani15172557","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T14:02:46Z","timestamp":1756735366000},"page":"2557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neo-X-Linked Chromosome Polymorphism: Cytogenetic Insights from Passalites nemorivagus (Mammalia, Cervidae)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3808-6095","authenticated-orcid":false,"given":"Raquel Muhlbeier","family":"Bonato","sequence":"first","affiliation":[{"name":"Deer Research and Conservation Center (NUPECCE), School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0369-1858","authenticated-orcid":false,"given":"Agda Maria","family":"Bernegossi","sequence":"additional","affiliation":[{"name":"Deer Research and Conservation Center (NUPECCE), School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eluzai Dinai Pinto","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Deer Research and Conservation Center (NUPECCE), School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2426-804X","authenticated-orcid":false,"given":"Halina","family":"Cernohorska","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Veterinary Research Institute, 621 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9076-8221","authenticated-orcid":false,"given":"Miluse","family":"Vozdova","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Veterinary Research Institute, 621 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7805-0265","authenticated-orcid":false,"given":"Jos\u00e9 Maur\u00edcio Barbanti","family":"Duarte","sequence":"additional","affiliation":[{"name":"Deer Research and Conservation Center (NUPECCE), School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0169-5347(01)02187-5","article-title":"Chromosomal Rearrangements and Speciation","volume":"16","author":"Rieseberg","year":"2001","journal-title":"Trends Ecol. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.tree.2010.07.008","article-title":"Chromosomal Speciation Revisited: Rearranging Theory with Pieces of Evidence","volume":"25","author":"Faria","year":"2010","journal-title":"Trends Ecol. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1755-8166-4-22","article-title":"The Genome Diversity and Karyotype Evolution of Mammals","volume":"4","author":"Graphodatsky","year":"2011","journal-title":"Mol. Cytogenet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1186\/1297-9686-39-5-583","article-title":"Chromosomal Control of Pig Populations in France: 2002\u20132006 Survey","volume":"39","author":"Ducos","year":"2007","journal-title":"Genet. Sel. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/brv.12213","article-title":"Chromosomal Polymorphism in Mammals: An Evolutionary Perspective","volume":"92","author":"Dobigny","year":"2017","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.4238\/vol9-3gmr822","article-title":"Different Responses to Doxorubicin-Induced Chromosome Aberrations in Brazilian Deer Species","volume":"9","author":"Duarte","year":"2010","journal-title":"Genet. Mol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1126\/science.168.3937.1364","article-title":"Indian Muntjac, Muntiacus muntjak: A Deer with a Low Diploid Chromosome Number","volume":"168","author":"Wurster","year":"1970","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0303-2647(90)90008-O","article-title":"Chromosomal Evolution in Cervidae","volume":"24","author":"Fontana","year":"1990","journal-title":"Biosystems"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1159\/000525592","article-title":"Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae","volume":"162","author":"Bernegossi","year":"2022","journal-title":"Cytogenet. Genome Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Proskuryakova, A.A., Ivanova, E.S., Makunin, A.I., Larkin, D.M., Ferguson-Smith, M.A., Yang, F., Uphyrkina, O.V., Perelman, P.L., and Graphodatsky, A.S. (2023). Comparative Studies of X Chromosomes in Cervidae Family. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-39088-4"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3897\/zookeys.1167.100577","article-title":"Revalidation of Passalites Gloger, 1841 for the Amazon Brown Brocket Deer P. nemorivagus (Cuvier, 1817) (Mammalia, Artiodactyla, Cervidae)","volume":"1167","author":"Vacari","year":"2023","journal-title":"ZooKeys"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.ympev.2008.07.009","article-title":"The Surprising Evolutionary History of South American Deer","volume":"49","author":"Duarte","year":"2008","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3897\/compcytogen.v7i1.4314","article-title":"Cytogenetic Description of the Amazonian Brown Brocket Mazama nemorivaga (Artiodactyla, Cervidae)","volume":"7","author":"Fiorillo","year":"2013","journal-title":"Comp. Cytogenet."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Galindo, D.J., Martins, G.S., Vozdova, M., Cernohorska, H., Kubickova, S., Bernegossi, A.M., Kadlcikova, D., Rubes, J., and Duarte, J.M. (2021). Chromosomal Polymorphism and Speciation: The Case of the Genus Mazama (Cetartiodactyla; Cervidae). Genes, 12.","DOI":"10.3390\/genes12020165"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/jhered\/esae031","article-title":"The Role of Conflict in the Formation and Maintenance of Variant Sex Chromosome Systems in Mammals","volume":"115","author":"Hughes","year":"2024","journal-title":"J. Hered."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s10641-011-9853-8","article-title":"Turnover of Sex Chromosomes and Speciation in Fishes","volume":"94","author":"Kitano","year":"2012","journal-title":"Environ. Biol. Fishes"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3198","DOI":"10.1111\/j.1558-5646.2012.01681.x","article-title":"The contribution of female meiotic drive to the evolution of neo-sex chromosomes","volume":"66","author":"Yoshida","year":"2012","journal-title":"Evolution"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/BF00285762","article-title":"Synaptonemal Complex of the Sex-Autosome Trivalent in a Male Indian Muntjac","volume":"82","author":"Pathak","year":"1981","journal-title":"Chromosoma"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1159\/000084212","article-title":"Polymorphic Karyotypes and Sex Chromosomes in the Tufted Deer (Elaphodus cephalophus): Cytogenetic Studies and Analyses of Sex Chromosome-Linked Genes","volume":"109","author":"Cao","year":"2005","journal-title":"Cytogenet. Genome Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1093\/jmammal\/gyac068","article-title":"Resurrection of the Genus Subulo Smith, 1827 for the Gray Brocket Deer, with Designation of a Neotype","volume":"104","author":"Bernegossi","year":"2023","journal-title":"J. Mammal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.4238\/2013.September.13.1","article-title":"Meiotic Pairing of B Chromosomes, Multiple Sexual System, and Robertsonian Fusion in the Red Brocket Deer Mazama americana (Mammalia, Cervidae)","volume":"12","author":"Aquino","year":"2013","journal-title":"Genet. Mol. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Peres, P.H.F., Luduv\u00e9rio, D.J., Bernegossi, A.M., Galindo, D.J., Nascimento, G.B., Oliveira, M.L., Sandoval, E.D.P., Vozdova, M., Kubickova, S., and Cernohorska, H. (2021). Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777). Front. Genet., 12.","DOI":"10.3389\/fgene.2021.742870"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/s00412-004-0292-6","article-title":"Viability of X-Autosome Translocations in Mammals: An Epigenomic Hypothesis from a Rodent Case-Study","volume":"113","author":"Dobigny","year":"2004","journal-title":"Chromosoma"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00412-007-0140-6","article-title":"Dissection of a Y-Autosome Translocation in Cryptomys hottentotus (Rodentia, Bathyergidae) and Implications for the Evolution of a Meiotic Sex Chromosome Chain","volume":"117","author":"Deuve","year":"2008","journal-title":"Chromosoma"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Searle, J.B., Polly, P.D., and Zima, J. (2019). Meiosis and Fertility Associated with Chromosomal Heterozygosity. Shrews, Chromosomes and Speciation, Cambridge University Press.","DOI":"10.1017\/9780511895531"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s00412-015-0566-1","article-title":"Protein Markers of Synaptic Behavior and Chromatin Remodeling of the Neo-XY Body in Phyllostomid Bats","volume":"125","author":"Rahn","year":"2016","journal-title":"Chromosoma"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s10577-016-9524-x","article-title":"Meiotic Behaviour of Evolutionary Sex-Autosome Translocations in Bovidae","volume":"24","author":"Vozdova","year":"2016","journal-title":"Chromosome Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s10344-020-1367-2","article-title":"Faecal DNA and Camera Traps Detect an Evolutionarily Significant Unit of the Amazonian Brocket Deer in the Brazilian Atlantic Forest","volume":"66","author":"Gatti","year":"2020","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/biolre\/ioz031","article-title":"Breakthroughs and New Horizons in Reproductive Biology of Rare and Endangered Animal Species","volume":"101","author":"Comizzoli","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10344-016-1040-y","article-title":"First Live Offspring of Amazonian Brown Brocket Deer (Mazama nemorivaga) Born by Artificial Insemination","volume":"62","author":"Oliveira","year":"2016","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/bio.2023.0030","article-title":"A Method to Freeze Skin Samples for Cryobanks: A Test of Some Cryoprotectants for an Endangered Deer","volume":"22","author":"Oliveira","year":"2024","journal-title":"Biopreserv. Biobank."},{"key":"ref_32","unstructured":"Verma, R., and Babu, A. (1995). Human Chromosomes: Principles & Techniques, McGraw-Hill, Inc.. [2nd ed.]."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/0888-7543(92)90147-K","article-title":"Degenerate Oligonucleotide-Primed PCR: General Amplification of Target DNA by a Single Degenerate Primer","volume":"13","author":"Telenius","year":"1992","journal-title":"Genomics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1023\/A:1020914702767","article-title":"The Use of Laser Microdissection for the Preparation of Chromosome-Specific Painting Probes in Farm Animals","volume":"10","author":"Kubickova","year":"2002","journal-title":"Chromosome Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1159\/000503082","article-title":"Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae","volume":"159","author":"Vozdova","year":"2019","journal-title":"Cytogenet. Genome Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1098\/rstb.2000.0556","article-title":"B-Chromosome Evolution","volume":"355","author":"Camacho","year":"2000","journal-title":"Phil. Trans. R. Soc. Lond. B"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1159\/000298819","article-title":"Elucidating the Evolution of the Red Brocket Deer Mazama americana Complex (Artiodactyla; Cervidae)","volume":"128","author":"Abril","year":"2010","journal-title":"Cytogenet. Genome Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s13039-023-00669-7","article-title":"Characteristics and Clinical Evaluation of X Chromosome Translocations","volume":"16","author":"Huang","year":"2023","journal-title":"Mol. Cytogenet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s13353-024-00861-4","article-title":"Comparative Karyotype Analysis of the Red Brocket Deer (M. americana Sensu Lato and M. rufa) Complex: Evidence of Drastic Chromosomal Evolution and Implications on Speciation Process","volume":"65","author":"Bernegossi","year":"2024","journal-title":"J. Appl. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1139\/cjz-2022-0037","article-title":"Cytogenetic, Molecular, and Morphological Characterization of Odocoileus pandora (Merriam, 1901) (Artiodactyla, Cervidae)","volume":"101","author":"Sandoval","year":"2023","journal-title":"Can. J. Zool."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3390\/taxonomy4010002","article-title":"Description of Bisbalus, a New Genus for the Gray Brocket, Mazama cita Osgood, 1912 (Mammalia, Cervidae), as a Step to Solve the Neotropical Deer Puzzle","volume":"4","author":"Sandoval","year":"2024","journal-title":"Taxonomy"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"192","DOI":"10.12933\/therya-24-4913","article-title":"Molecular Cytogenetics Markers Reveal the Existence of a Cryptic Complex of Mazama temama Species","volume":"15","author":"Sandoval","year":"2024","journal-title":"Therya"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1071\/ZO9890331","article-title":"Marsupial Cytogenetics","volume":"37","author":"Hayman","year":"1989","journal-title":"Aust. J. Zool."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s003359900459","article-title":"Comparative Chromosome Painting between Two Marsupials: Origins of an XX\/XY1Y2 Sex Chromosome System","volume":"8","author":"Toder","year":"1997","journal-title":"Mamm. Genome"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1111\/jeb.14039","article-title":"Sex Chromosome Systems in Neotropical Primates: What Have We Learnt so Far from Cytogenetics and Genomics?","volume":"35","author":"Steinberg","year":"2022","journal-title":"J. Evol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13072-023-00493-8","article-title":"Premature Ovarian Insufficiency Is Associated with Global Alterations in the Regulatory Landscape and Gene Expression in Balanced X-Autosome Translocations","volume":"16","author":"Favilla","year":"2023","journal-title":"Epigenet. Chromatin"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s10764-023-00352-z","article-title":"Genetic Diversity and Insights into the Distribution of Brown Howler Monkeys (Alouatta guariba Group) (Atelidae, Alouattinae)","volume":"44","author":"Povill","year":"2023","journal-title":"Int. J. Primatol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Iannuzzi, A., Parma, P., and Iannuzzi, L. (2021). Chromosome Abnormalities and Fertility in Domestic Bovids: A Review. Animals, 11.","DOI":"10.3390\/ani11030802"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Charlesworth, B., and Wall, J.D. (1999). Inbreeding, Heterozygote Advantage and the Evolution of Neo-X and Neo-Y Sex Chromosomes. Proc. R. Soc. B Biol. Sci., 266.","DOI":"10.1098\/rspb.1999.0603"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"735","DOI":"10.2307\/2408385","article-title":"Sex Chromosomes and the Evolution of Sexual Dimorphism","volume":"38","author":"Rice","year":"1984","journal-title":"Evolution"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1159\/000014976","article-title":"A Molecular Cytogenetic Analysis of X Chromosome Repatterning in the Bovidae: Transpositions, Inversions, and Phylogenetic Inference","volume":"80","author":"Robinson","year":"1998","journal-title":"Cytogenet. Cell Genet."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Proskuryakova, A.A., Kulemzina, A.I., Perelman, P.L., Makunin, A.I., Larkin, D.M., Farr\u00e9, M., Kukekova, A.V., Lynn Johnson, J., Lemskaya, N.A., and Beklemisheva, V.R. (2017). X Chromosome Evolution in Cetartiodactyla. Genes, 8.","DOI":"10.3390\/genes8090216"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1159\/000133085","article-title":"The Chromosomes of Tufted Deer (Elaphodus cephalophus)","volume":"56","author":"Shi","year":"1991","journal-title":"Cytogenet. Cell Genet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1002\/zoo.21223","article-title":"Chromosomal Variation and Perinatal Mortality in San Diego Zoo Soemmerring\u2019s Gazelles","volume":"34","author":"Steiner","year":"2015","journal-title":"Zoo. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/BF01539454","article-title":"Fine Structure of the XY Body in the XY1Y2 Trivalent of the Bat Artibeus lituratus","volume":"2","author":"Solari","year":"1994","journal-title":"Chromosome Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1007\/s10577-010-9146-7","article-title":"Meiotic Analysis of XX\/XY and Neo-XX\/XY Sex Chromosomes in Phyllostomidae by Cross-Species Chromosome Painting Revealing a Common Chromosome 15-XY Rearrangement in Stenodermatinae","volume":"18","author":"Noronha","year":"2010","journal-title":"Chromosome Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00087114.2004.10589365","article-title":"Meiotic Analyses of the Sex Chromosomes in Carolliinae-Phyllostomidae (Chiroptera): NOR Separates the XY1Y2 into Two Independent Parts","volume":"57","author":"Noronha","year":"2004","journal-title":"Caryologia"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1159\/000063030","article-title":"X-Autosome Translocations, Meiotic Synapsis, Chromosome Evolution and Speciation","volume":"96","author":"Ashley","year":"2002","journal-title":"Cytogenet. Genome Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"143","DOI":"10.3897\/zookeys.958.50300","article-title":"Designation of a Neotype for Mazama americana (Artiodactyla, Cervidae) Reveals a Cryptic New Complex of Brocket Deer Species","volume":"958","author":"Sandoval","year":"2020","journal-title":"ZooKeys"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Cursino, M.S., Salviano, M.B., Abril, V.V., Zanetti, E.d.S., and Duarte, J.M.B. (2014). The Role of Chromosome Variation in the Speciation of the Red Brocket Deer Complex: The Study of Reproductive Isolation in Females. BMC Evol. Biol., 14.","DOI":"10.1186\/1471-2148-14-40"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1093\/biolre\/iox041","article-title":"Intraspecific Chromosome Polymorphisms Can Lead to Reproductive Isolation and Speciation: An Example in Red Brocket Deer (Mazama americana)","volume":"96","author":"Salviano","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/190372a0","article-title":"Gene Action in the X-Chromosome of the Mouse (Mus. musculus L.)","volume":"190","author":"Lyon","year":"1961","journal-title":"Nature"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1111\/and.12362","article-title":"Sperm Aneuploidy in Infertile Male Patients: A Systematic Review of the Literature","volume":"47","author":"Chatziparasidou","year":"2015","journal-title":"Andrologia"},{"key":"ref_64","first-page":"e64734","article-title":"Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization","volume":"194","author":"Nadesapillai","year":"2023","journal-title":"J. Vis. Exp."},{"key":"ref_65","unstructured":"Bonato, R.M., Bernegossi, A.M., Sandoval, E.D.P., Cernohorska, H., Vozdova, M., and Duarte, J.M.B. (2025, January 10\u201313). Gen\u00e9tica da conserva\u00e7\u00e3o: Polimorfismo sexual no Veado-roxo e implica\u00e7\u00f5es para o manejo reprodutivo em cativeiro. Proceedings of the Anais do 48\u00ba Congresso da Associa\u00e7\u00e3o de Zool\u00f3gicos e Aqu\u00e1rios do Brasil (AZAB), Bras\u00edlia, Brazil."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/17\/2557\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:36:12Z","timestamp":1760034972000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/17\/2557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,30]]},"references-count":65,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["ani15172557"],"URL":"https:\/\/doi.org\/10.3390\/ani15172557","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,8,30]]}}}