{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:17:24Z","timestamp":1790453844722,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:00:00Z","timestamp":1759017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Animals"],"abstract":"<jats:p>During the grain and cereal production process, whether during harvesting, processing, or storage, errors can occur, compromising product quality and potentially leading to contamination by fungi, which produce toxic substances known as mycotoxins. When fed to animals, these contaminated grains and cereals can cause several negative effects on animal health, impacting their production performance, including immunosuppression, hepatotoxicity, nephrotoxicity, and reproductive problems. To minimize the problems caused by mycotoxins, anti-mycotoxin additives, also known as adsorbents, are used. These are inert materials that bind to mycotoxins and are excreted in feces, preventing their action within the animal\u2019s body. Therefore, the objective of this study was to evaluate the effectiveness of an anti-mycotoxin product based on bentonite, activated charcoal, milk thistle extract, and yeast cell wall in the diet of mycotoxin-contaminated lambs on animal health and performance. Thirty lambs were divided into three homogeneous groups: control (without mycotoxins or additives), mycotoxin (with mycotoxins), and anti-mycotoxin (mycotoxins and additive). The mycotoxins used for feed contamination were aflatoxin (AFLA) (200 ppb), fumonisin (FUMO) (15 ppm), zearalenone (ZEA) (500 ppb), and deoxynivalenol (DON) (1.5 ppm). The anti-mycotoxin additive was used at a dose of 1 kg\/ton of concentrate. Parameters of zootechnical performance, hematological profile, serum biochemistry, and oxidative status were evaluated. The group that ingested the contaminated concentrate with mycotoxin had a lower average daily weight gain (ADG) when compared to the control and anti-mycotoxin groups. Ingestion of a mycotoxin-contaminated diet increased the activity of aspartate aminotransferase and gamma-glutamyltransferase, which are indicators of liver damage. However, when the anti-mycotoxin additive was used, the increase in these enzymes was modest and lower than in the mycotoxin group. Ingestion of a mycotoxin-containing concentrate increased levels of oxidative stress biomarkers such as reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), myeloperoxidase (MPO), and reduced glutathione (GST), demonstrating that the mycotoxin challenge was effective in causing oxidative stress. However, when the diet was contaminated with mycotoxins and supplemented with the anti-mycotoxin additive, the levels of ROS and TBARS were similar to those of the negative control group. We concluded that adding the anti-mycotoxin product to the lambs\u2019 diets prevented or minimized the problems caused by mycotoxin consumption, allowing these lambs to have ADG, and feed efficiency similar to the control group.<\/jats:p>","DOI":"10.3390\/ani15192835","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T11:40:52Z","timestamp":1759146052000},"page":"2835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Combination of Anti-Mycotoxin Additive in Diet Contaminated with Multiple Mycotoxins (Aflatoxin, Fumonisin, Zearalenone and Deoxynivalenol): Effects on Performance and Health of Lambs"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3987-3661","authenticated-orcid":false,"given":"Suelyn de Oliveira","family":"Marques","sequence":"first","affiliation":[{"name":"Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapec\u00f3 89815-630, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9734-6902","authenticated-orcid":false,"given":"Guilherme Luiz","family":"Deolindo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Biologia Molecular, Universidade do Estado de Santa Catarina, Lages 88520-000, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5970-7703","authenticated-orcid":false,"given":"Andrei Lucas Rebelatto","family":"Brunetto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Zootecnia, Universidade do Estado de Santa Catarina, Chapec\u00f3 89815-630, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lara Amaral","family":"da Veiga","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapec\u00f3 89815-630, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato Santos","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapec\u00f3 89815-630, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0544-8632","authenticated-orcid":false,"given":"Eduardo Micotti","family":"Da Gloria","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Luiz de Queiroz College of Agriculture, University of S\u00e3o Paulo, Piracicaba 13418-900, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilnei Bruno","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Biologia Molecular, Universidade do Estado de Santa Catarina, Lages 88520-000, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarete Dulce","family":"Bagatini","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal Fronteira Sul, Chapec\u00f3 89812-000, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandro Schafer","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapec\u00f3 89815-630, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,28]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Micotoxinas em produtos de origem animal","volume":"30","author":"Dias","year":"2018","journal-title":"Rev. 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