{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T09:20:37Z","timestamp":1792833637198,"version":"4.5.0"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biog\u00e9nesis Bag\u00f3"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Two experiments evaluated the effects of copper (Cu) and zinc (Zn) injection on body weight (BW), body condition score (BCS), pregnancy rate, ovarian traits, and antioxidant and inflammatory responses of beef heifers. In Exp. 1, 29 heifers were assigned to having saline or CuZn (a solution containing 15 and 50 mg\/mL of Cu and Zn) subcutaneously injected (5 mL\/heifer) 9 days before artificial insemination. Exp. 2 was conducted to increase the statistical power to evaluate pregnancy rate, and 283 heifers were assigned to either the saline or CuZn group. In Exp. 1, CuZn heifers tended (p \u2264 0.10) to gain more BW and to have greater corpus luteum size and plasma concentration of glutathione peroxidase. No effects of treatment were detected (p \u2265 0.18) for BCS; estrus score; serum concentration of Cu, Zn, and cortisol; and plasma concentration of haptoglobin, ceruloplasmin, and superoxide dismutase. In Exp. 2, CuZn heifers had greater (p &lt; 0.01) BW and serum Cu. The CuZn heifers with low BCS had greater (p \u2264 0.05) estrus scores and tended (p = 0.10) to have greater pregnancy rates. Thus, injecting CuZn may be an effective strategy to enhance growth, reproductive performance, and antioxidant responses in heifers, especially when their BCS is below 5.<\/jats:p>","DOI":"10.3390\/ani15192926","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:19:26Z","timestamp":1760015966000},"page":"2926","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Parenteral Copper and Zinc Administration on Reproduction, Inflammation, and Antioxidant Responses of Bos indicus Beef Heifers"],"prefix":"10.3390","volume":"15","author":[{"given":"Luana Gomes da","family":"Silva","sequence":"first","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9050-6990","authenticated-orcid":false,"given":"Marcelo","family":"Vedovatto","sequence":"additional","affiliation":[{"name":"Dean Lee Research and Extension Center, Louisiana State University, Alexandria, LA 71302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-2577","authenticated-orcid":false,"given":"Juliana","family":"Ranches","sequence":"additional","affiliation":[{"name":"Eastern Oregon Agricultural Research Center, Oregon State University, Burns, OR 97720, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edilane Costa","family":"Martins","sequence":"additional","affiliation":[{"name":"Eastern Oregon Agricultural Research Center, Oregon State University, Burns, OR 97720, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Fellipe","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Hill Farm Research Station, Louisiana State University, Homer, LA 71040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0437-3651","authenticated-orcid":false,"given":"Eduardo de Assis","family":"Lima","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6727-8603","authenticated-orcid":false,"given":"Luiz Carlos Louzada","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Cia Pecu\u00e1ria Assessoria, Campo Grande 79003-140, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7806-9396","authenticated-orcid":false,"given":"Willian Vaniel Alves dos","family":"Reis","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9868-0256","authenticated-orcid":false,"given":"Gumercindo Loriano","family":"Franco","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"ref_1","unstructured":"Valadares Filho, S.C., Costa e Silva, L.F., Gionbelli, M.P., Rotta, P.P., Marcondes, M.I., Chizzotti, M.L., and Prados, L.F. 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