{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:50Z","timestamp":1787027510563,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Poly Gain Pte. Ltd."},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"crossref","award":["RGNS 65-091"],"award-info":[{"award-number":["RGNS 65-091"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Coccidiosis, caused by Eimeria spp., is a major economic burden in poultry production, prompting growing interest in natural alternatives to synthetic anticoccidials. This study evaluated the use of Polygain\u2122, a sugarcane-derived polyphenol-rich feed material, as a natural anticoccidial in broiler chickens experimentally challenged with Eimeria tenella, E. maxima, and E. acervulina. A total of 144 Ross 308 chicks were allocated to six groups: uninfected\u2013untreated control, infected\u2013untreated control, infected plus nicarbazin + narasin, and three Polygain\u2122 treatment groups (250, 500, and 1000 ppm). Birds were orally challenged with mixed oocysts on day 14. Parameters assessed included body weight gain (BWG), lesion scores (LS), oocyst per gram (OPG), and calculated indices such as relative oocyst production (ROP), reduction in lesion score (RLS), percent optimum anticoccidial activity (POAA), and anticoccidial index (ACI). Polygain\u2122 reduced intestinal lesions (p &lt; 0.05), particularly in the caecum, with complete lesion resolution observed at 1000 ppm by 21 days post-infection. Polygain\u2122 ACI values (143\u2013146) were limited in comparison to nicarbazin + narasin treatment (ACI 160). These findings demonstrate that Polygain\u2122 supplementation confers measurable protective dose related effects against coccidiosis without impairing growth performance, supporting its potential as a natural feed material for integrated coccidiosis management.<\/jats:p>","DOI":"10.3390\/ani15213130","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:16:24Z","timestamp":1761729384000},"page":"3130","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Polygain\u2122 Supplementation on Growth Performance, Lesion Severity, and Oocyst Shedding in Eimeria-Challenged Broiler Chickens"],"prefix":"10.3390","volume":"15","author":[{"given":"Thalia Marina","family":"Llalla Vidal","sequence":"first","affiliation":[{"name":"The Product Makers, Melbourne, VIC 3173, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siraprapa","family":"Boobphahom","sequence":"additional","affiliation":[{"name":"Research Centre for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4935-644X","authenticated-orcid":false,"given":"Suttitas","family":"Tongkamsai","sequence":"additional","affiliation":[{"name":"Research Centre for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand"},{"name":"Department of Veterinary Medicine, Faculty of Veterinary Science, Rajamangala University of Technology Tawan-Ok, Chonburi 20110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7455-5162","authenticated-orcid":false,"given":"Matthew","family":"Flavel","sequence":"additional","affiliation":[{"name":"The Product Makers, Melbourne, VIC 3173, Australia"},{"name":"Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC 3083, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","article-title":"Re-calculating the cost of coccidiosis in chickens","volume":"51","author":"Blake","year":"2020","journal-title":"Vet. 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