{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:30:37Z","timestamp":1793727037968,"version":"4.6.1"},"reference-count":67,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA-National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2024-38821-42115"],"award-info":[{"award-number":["2024-38821-42115"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Consisting of phospholipids, sperm membranes surround the head and tail, playing essential roles in maintaining cellular structural integrity and functions. Their characteristics directly influence sperm fertility and cryopreservation outcomes. This minireview provides a summary of how sperm fertility and freezability are affected by the characteristics of its cell membranes. The primary emphasis is on the molecular and cellular anatomy as well as the physiology of sperm membranes and their attributes associated with fertility determinants or biomarkers for fertility and freezability. It also explores how this knowledge can guide the development of extenders to improve sperm freezability and enhance reproductive technologies in mammals. By providing integrity, fluidity, and selective permeability, the membranes play vitally important roles in sperm motility, which is required for successful fertilization. Cryopreservation, which involves freezing and thawing of sperm for storage or ART, alters the integrity and functionality of the sperm membranes. Sperm freezability, its viability following freezing and thawing, is influenced by several properties of the sperm cell membranes, such as lipid composition, cholesterol content, and structures and functions of the membrane proteins. This review provides concise information about the nature of sperm membranes. It highlights the importance of understanding specific biophysical and biochemical features, including lipid composition, protein distribution, and membrane phase behavior. Particular attention is given to parameters such as the cholesterol\u2013phospholipid ratio and membrane phase transition temperature (Tm). A deeper understanding of these factors can contribute to the identification of reliable fertility biomarkers and the optimization of cryopreservation techniques used in ART and animal breeding programs. Furthermore, this review underscores the need for comprehensive investigations into the molecular and cellular architecture of sperm cells. Such studies are essential for advancing both fundamental and applied aspects of reproductive biology in food-producing animals, endangered species, and humans.<\/jats:p>","DOI":"10.3390\/ani15223248","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T11:47:27Z","timestamp":1762775247000},"page":"3248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sperm Cell Membranes of Bulls and Bucks Associated with Sperm Fertility and Freezability"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5468-0844","authenticated-orcid":false,"given":"Seher","family":"Simsek","sequence":"first","affiliation":[{"name":"Ministry of Agriculture and Forestry, Republic of T\u00fcrkiye, General Directorate of Livestock, Ankara 06060, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Hitit","sequence":"additional","affiliation":[{"name":"Cooperative Agricultural Research Center, College of Agriculture, Food, and Natural Resources, Prairie View A&M University, Prairie View, TX 77446, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8432-7347","authenticated-orcid":false,"given":"Mustafa","family":"Bodu","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42075, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erdogan","family":"Memili","sequence":"additional","affiliation":[{"name":"Cooperative Agricultural Research Center, College of Agriculture, Food, and Natural Resources, Prairie View A&M University, Prairie View, TX 77446, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hotea, I., Dragomirescu, M., Berbecea, A., and Radulov, I. (2023). The Role of Nutrition in Enhancing Sustainability in Sheep Production. Sheep Farming\u2014Sustainability from Traditional to Precision Production, IntechOpen.","DOI":"10.5772\/intechopen.113938"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1017\/S1751731112001954","article-title":"The roles of livestock in developing countries","volume":"7","author":"Herrero","year":"2013","journal-title":"Animal"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.5713\/ab.21.0072","article-title":"Semen evaluation: Methodological advancements in sperm quality-specific fertility assessment\u2014A review","volume":"34","author":"Tanga","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107643","DOI":"10.1016\/j.anireprosci.2024.107643","article-title":"Harnessing the value of fertility biomarkers in bull sperm for buck sperm","volume":"272","author":"Bodu","year":"2025","journal-title":"Anim. Reprod. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"106849","DOI":"10.1016\/j.anireprosci.2021.106849","article-title":"Assessment of sperm motility in livestock: Perspectives based on sperm swimming conditions in vivo","volume":"246","author":"Waberski","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Carvalho, F.E., Ferraz, J.B.S., Pedrosa, V.B., Matos, E.C., Eler, J.P., Silva, M.R., Guimar\u00e3es, J.D., Bussiman, F.O., Silva, B.C.A., and Can\u00e7ado, F.A. (2023). Genetic parameters for various semen production and quality traits and indicators of male and female reproductive performance in Nellore cattle. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09216-5"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Khan, M.Z., Chen, W., Naz, S., Liu, X., Liang, H., Chen, Y., Kou, X., Liu, Y., Ashraf, I., and Han, Y. (2024). Determinant genetic markers of semen quality in livestock. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1456305"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.22271\/veterinary.2023.v8.i5Sc.732","article-title":"Management strategies for improving production and reproduction performance of sheep and goat","volume":"8","author":"Kalwani","year":"2023","journal-title":"Int. J. Vet. Sci. Anim. Husb."},{"key":"ref_9","unstructured":"Salisbury, G., and VanDemark, N. (1961). Physiology of Reproduction and Artificial \u0130nsemination of Cattle, W. H. Freeman & Company."},{"key":"ref_10","unstructured":"Zammit, S. (2022). Physical Activity and Semen Quality Parameters in Adults. [Bachelor\u2019s Thesis, University of Malta]."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.theriogenology.2008.05.031","article-title":"The effect of nutrition on sexual development of bulls","volume":"70","author":"Barth","year":"2008","journal-title":"Theriogenology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3390\/ruminants4010003","article-title":"Enhancing Animal Production through Smart Agriculture: Possibilities, Hurdles, Resolutions, and Advantages","volume":"4","author":"Dayoub","year":"2024","journal-title":"Ruminants"},{"key":"ref_13","unstructured":"Dyce, K.M., Sack, W.O., and Wensing, C.J.G. (2009). Textbook of Veterinary Anatomy-E-Book, Elsevier Health Sciences."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0074-7696(08)62688-1","article-title":"The Structure of the Mammalian Spermatozoon","volume":"Volume 7","author":"Bourne","year":"1958","journal-title":"International Review of Cytology"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Leung, E.T.Y., Lee, B.K.M., Lee, C.-L., Tian, X., Lam, K.K.W., Li, R.H.W., Ng, E.H.Y., Yeung, W.S.B., Ou, J.-P., and Chiu, P.C.N. (2023). The role of spermatozoa-zona pellucida interaction in selecting fertilization-competent spermatozoa in humans. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1135973"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Skowronek, M.F., Pietroroia, S., de Cola, G., Ramos, M., Silvera, D., Casanova, G., Lecumberry, F., Cassina, A., and Sapiro, R. (2025). Mitochondrial morphology in fertile and infertile men: Image processing and morphometric analysis of the sperm midpiece. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1609081"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Costa, J., Braga, P.C., Rebelo, I., Oliveira, P.F., and Alves, M.G. (2023). Mitochondria Quality Control and Male Fertility. Biology, 12.","DOI":"10.3390\/biology12060827"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"R163","DOI":"10.1530\/REP-13-0178","article-title":"Mitochondria functionality and sperm quality","volume":"146","author":"Amaral","year":"2013","journal-title":"Reproduction"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1002\/humu.20378","article-title":"Evidence for adaptive selection acting on the tRNA and rRNA genes of human mitochondrial DNA","volume":"27","author":"Wallace","year":"2006","journal-title":"Hum. Mutat."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/bs.ctdb.2024.10.008","article-title":"Chapter Seven\u2014The mammalian egg\u2019s zona pellucida, fertilization, and fertility","volume":"Volume 162","author":"Wassarman","year":"2025","journal-title":"Current Topics in Developmental Biology"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hopper, R.M. (2021). Bovine Reproduction, John Wiley & Sons.","DOI":"10.1002\/9781119602484"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1242\/jcs.051326","article-title":"Flagellar and ciliary beating: The proven and the possible","volume":"123","author":"Lindemann","year":"2010","journal-title":"J. Cell Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1016\/j.theriogenology.2009.07.009","article-title":"Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluation","volume":"72","author":"Marenjak","year":"2009","journal-title":"Theriogenology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"840","DOI":"10.4103\/1008-682X.186877","article-title":"A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method","volume":"18","author":"Hidalgo","year":"2016","journal-title":"Asian J. Androl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1080\/01485010151096405","article-title":"Phospholipid composition of human sperm and seminal plasma in relation to sperm fertility","volume":"46","author":"Gulaya","year":"2001","journal-title":"Arch. Androl."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bodu, M., Hitit, M., Woldesenbet, S., U\u011fur, M.R., Erdo\u011fan, Z., Greenwood, O.C., Murray, R.D., Cervantes, A.P., and Memili, E. (2025). Lipidomic Landscapes of Cryopreserved Sperm from Alpine and Spanish\u2013Creole Bucks. Animals, 15.","DOI":"10.3390\/ani15131897"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1093\/biolre\/ioaa140","article-title":"The enigmatic sperm proteins in mammalian fertilization: An overview","volume":"103","author":"Gahlay","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0093-691X(01)00674-4","article-title":"Current status of sperm cryopreservation: Why isn\u2019t it better?","volume":"57","author":"Medeiros","year":"2002","journal-title":"Theriogenology"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Falc\u00f3, M., S\u00e1ez-Espinosa, P., L\u00f3pez-Botella, A., Aizpurua, J., and G\u00f3mez-Torres, M.J. (2022). The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073929"},{"key":"ref_30","first-page":"4985","article-title":"Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1","volume":"125","author":"Satouh","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0093-691X(99)00239-3","article-title":"Fundamental aspects of sperm cryobiology: The importance of species and individual differences","volume":"53","author":"Holt","year":"2000","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0015-0282(16)54255-1","article-title":"Levels of cholesterol and phospholipids in freshly ejaculated sperm and Percoll-gradient-pelletted sperm from fertile and unexplained infertile men","volume":"55","author":"Sugkraroek","year":"1991","journal-title":"Fertil. Steril."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Khodadadi, E., and Moradi, M. (2023). Cholesterol concentration effect on bilayer membranes and its role in designing efficient liposomal drug delivery systems. Biophys. J., 122.","DOI":"10.1016\/j.bpj.2022.11.2015"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3512","DOI":"10.1021\/ar500260t","article-title":"The Structural Role of Cholesterol in Cell Membranes: From Condensed Bilayers to Lipid Rafts","volume":"47","author":"Krause","year":"2014","journal-title":"Acc. Chem. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1080\/08982104.2023.2226216","article-title":"Liposome bilayer stability: Emphasis on cholesterol and its alternatives","volume":"34","author":"Nsairat","year":"2024","journal-title":"J. Liposome Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100893","DOI":"10.1016\/j.mcpro.2024.100893","article-title":"Spatial Organization of the Sperm Cell Glycoproteome","volume":"24","author":"Ji","year":"2025","journal-title":"Mol. Cell. Proteom."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bravo, A., S\u00e1nchez, R., Zambrano, F., and Uribe, P. (2024). Exogenous Oxidative Stress in Human Spermatozoa Induces Opening of the Mitochondrial Permeability Transition Pore: Effect on Mitochondrial Function, Sperm Motility and Induction of Cell Death. Antioxidants, 13.","DOI":"10.3390\/antiox13060739"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e38591","DOI":"10.1016\/j.heliyon.2024.e38591","article-title":"Energy metabolism and spermatogenesis","volume":"10","author":"Rotimi","year":"2024","journal-title":"Heliyon"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Sugita, H., Takarabe, S., Kageyama, A., Kawata, Y., and Ito, J. (2024). Molecular Mechanism of Oocyte Activation in Mammals: Past, Present, and Future Directions. Biomolecules, 14.","DOI":"10.3390\/biom14030359"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4274\/jtgga.galenos.2023.2022-7-15","article-title":"The effect of dietary habits on oocyte\/sperm quality","volume":"24","author":"Pradas","year":"2023","journal-title":"J. Turk. Ger. Gynecol. Assoc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.theriogenology.2023.07.009","article-title":"Comparative analysis of different metabolites in semen of Guanzhong dairy goats with different motility rates","volume":"210","author":"Yuan","year":"2023","journal-title":"Theriogenology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S1359-6101(01)00013-2","article-title":"Molecules that function in the steps of fertilization","volume":"12","author":"Singson","year":"2001","journal-title":"Cytokine Growth Factor. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1093\/humupd\/dmad026","article-title":"Genetic mechanisms of fertilization failure and early embryonic arrest: A comprehensive review","volume":"30","author":"Wei","year":"2023","journal-title":"Hum. Reprod. Update"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Merc, V., Frolikova, M., and Komrskova, K. (2021). Role of Integrins in Sperm Activation and Fertilization. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111809"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Bodu, M., Hitit, M., Donmez, H., Kaya, A., Ugur, M.R., and Memili, E. (2025). Exploration of Small Non-Coding RNAs as Molecular Markers of Ram Sperm Fertility. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062690"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.theriogenology.2013.05.024","article-title":"Variation in lipid profiles within semen compartments\u2014The bovine model of aging","volume":"80","author":"Mahgrefthe","year":"2013","journal-title":"Theriogenology"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Shan, S., Xu, F., Hirschfeld, M., and Brenig, B. (2021). Sperm Lipid Markers of Male Fertility in Mammals. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22168767"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bodu, M., Hitit, M., Greenwood, O.C., Murray, R.D., and Memili, E. (2025). Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1554771"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"106884","DOI":"10.1016\/j.anireprosci.2021.106884","article-title":"\u201cFine feathers make fine birds\u201d\u2014The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction","volume":"246","author":"Gautier","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.14202\/vetworld.2021.1220-1233","article-title":"Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders","volume":"14","author":"Bustani","year":"2021","journal-title":"Vet. World"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.theriogenology.2023.03.023","article-title":"Ram sperm cryopreservation disrupts metabolism of unsaturated fatty acids","volume":"204","author":"Ofosu","year":"2023","journal-title":"Theriogenology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0011-2240(92)90024-V","article-title":"Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes","volume":"29","author":"Parks","year":"1992","journal-title":"Cryobiology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.anireprosci.2015.11.014","article-title":"Enriching membrane cholesterol improves stability and cryosurvival of buffalo spermatozoa","volume":"164","author":"Rajoriya","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1530\/rep.1.01049","article-title":"Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm","volume":"131","author":"Waterhouse","year":"2006","journal-title":"Reproduction"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4716","DOI":"10.3390\/cimb45060300","article-title":"Sperm Cryopreservation Today: Approaches, Efficiency, and Pitfalls","volume":"45","author":"Ozimic","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"146","DOI":"10.4103\/2305-0500.346093","article-title":"Effect of cholesterol-loaded cyclodextrin enriched extenders on the quality of prefrozen and frozen buffalo semen","volume":"11","author":"Mostafa","year":"2022","journal-title":"Asian Pac. J. Reprod."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107547","DOI":"10.1016\/j.anireprosci.2024.107547","article-title":"Insights into crucial molecules and protein channels involved in pig sperm cryopreservation","volume":"269","year":"2024","journal-title":"Anim. Reprod. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0011-2240(90)90009-S","article-title":"Cold-induced ultrastructural changes in bull and boar sperm plasma membranes","volume":"27","author":"Chen","year":"1990","journal-title":"Cryobiology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3836","DOI":"10.3168\/jds.S0022-0302(91)78576-7","article-title":"Effects of Egg Yolk-Citrate and Milk Extenders on Chromatin Structure and Viability of Cryopreserved Bull Sperm","volume":"74","author":"Karabinus","year":"1991","journal-title":"J. Dairy. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cryobiol.2016.11.001","article-title":"Optimization of the cryopreservation of dromedary camel semen: Cryoprotectants and their concentration and equilibration times","volume":"74","author":"Malo","year":"2017","journal-title":"Cryobiology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"107572","DOI":"10.1016\/j.anireprosci.2024.107572","article-title":"Thawing of cryopreserved sperm from domestic animals: Impact of temperature, time, and addition of molecules to thawing\/insemination medium","volume":"268","author":"Pezo","year":"2024","journal-title":"Anim. Reprod. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s11259-024-10573-z","article-title":"Impacts of adding sucrose or trehalose to extenders with different glycerol concentrations on freezablility and fertility of buffalo bull semen","volume":"49","author":"Khalil","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Esmeryan, K.D., Fedchenko, Y.I., and Chaushev, T.A. (2025). Probing the impact of commercial cryoprotectants and freezing technique on the motility of human spermatozoa cryopreserved onto extremely water-repellent soot-coated surfaces. Cryobiology, 118.","DOI":"10.1016\/j.cryobiol.2025.105195"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"0963689721999617","DOI":"10.1177\/0963689721999617","article-title":"Cryopreservation: An Overview of Principles and Cell-Specific Considerations","volume":"30","author":"Whaley","year":"2021","journal-title":"Cell Transplant."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.1007\/s10616-016-0027-6","article-title":"Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa","volume":"68","author":"Shah","year":"2016","journal-title":"Cytotechnology"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.theriogenology.2010.10.032","article-title":"Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility","volume":"75","author":"Kirkwood","year":"2011","journal-title":"Theriogenology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"106904","DOI":"10.1016\/j.anireprosci.2021.106904","article-title":"Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep","volume":"246","author":"Yeste","year":"2022","journal-title":"Anim. 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