{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:46:17Z","timestamp":1763117177116,"version":"3.45.0"},"reference-count":42,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Rungen Biotechnology Co., Ltd.","award":["20230715"],"award-info":[{"award-number":["20230715"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Soybean meal causes health issues in piglets due to the presence of antigenic proteins. Although enzymatic treatment can break down antigenic proteins, subsequent high-temperature drying may impair amino acid (AA) digestibility via the Maillard reaction. This study evaluated whether the air-drying temperature affects the ileal AA digestibility of a novel reduced-sugar enzyme-treated soybean meal (ESM) in 25 kg pigs, using fishmeal as a high-digestibility reference. In two trials using pigs fitted with simple T-cannulas in the terminal ileum, ileal digestibility was assessed. In trial 1, a replicated 3 \u00d7 3 Latin square design with three diets (fishmeal, ESM, and a nitrogen-free diet; two pigs per diet) and three periods were used per square. Fishmeal showed greater apparent (82.50% vs. 45.01%) and standardized (86.60% vs. 48.86%) digestibility of crude protein and all amino acids than ESM dried at 130 \u00b0C. In trial 2, eight pigs were allocated to two diets in a two-period crossover design to compare the AA digestibility of ESM dried at high (130 \u00b0C; HtESM) and low (80 \u00b0C; LtESM) temperatures. LtESM exhibited greater apparent (82.24% vs. 52.40%) and standardized (86.37% vs. 56.47%) digestibility of crude protein and more amino acids than HtESM. Collectively, the drying temperature critically determined the AA digestibility of ESM, irrespective of its reducing sugar content.<\/jats:p>","DOI":"10.3390\/ani15223288","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:07:03Z","timestamp":1763114823000},"page":"3288","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Drying Temperature Dictates Ileal Amino Acid Digestibility of Enzyme-Treated Soybean Meal in 25 kg Pigs"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3500-3391","authenticated-orcid":false,"given":"Xianyi","family":"Tan","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Bioengineering Institute, Sichuan Runge Biotechnology Co., Ltd., Deyang 618200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Liu","sequence":"additional","affiliation":[{"name":"Bioengineering Institute, Sichuan Runge Biotechnology Co., Ltd., Deyang 618200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Bioengineering Institute, Sichuan Runge Biotechnology Co., Ltd., Deyang 618200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8348-4199","authenticated-orcid":false,"given":"Yong","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9241-6614","authenticated-orcid":false,"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2972-9677","authenticated-orcid":false,"given":"Yunxiang","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.2527\/1990.6861790x","article-title":"Transient hypersensitivity to soybean meal in the early-weaned pig","volume":"68","author":"Li","year":"1990","journal-title":"J. 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