{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:00:32Z","timestamp":1780066832747,"version":"3.54.0"},"reference-count":22,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022271","name":"Faculty of Veterinary Medicine, Kasetsart University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022271","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study determined the optimal concentration of hydrogen peroxide (H2O2) for inducing oxidative stress in bovine oocytes prior to in vitro maturation (IVM). Ovaries were collected from local abattoirs, and cumulus\u2013oocyte complexes (COCs) were aspirated, selected, and allocated into four groups, exposed to 0, 50, 75, or 100 \u00b5M H2O2, respectively, for 1 h in collecting medium. This was followed by IVM in TCM-199 at 38.5 \u00b0C in humidified atmosphere of 5% CO2 for 23 h. Nuclear maturation was assessed by aceto-orcein staining. Exposure to increasing concentrations of H2O2 resulted in a clear, dose-dependent trend of decreased nuclear maturation rates. The control group (0 \u00b5M) exhibited the highest proportion of oocytes reaching the metaphase II (MII) stage (69.23 \u00b1 8.45%), which remained comparable at 50 \u00b5M (67.50 \u00b1 12.29%). A mild, though not statistically significant, decrease was observed at 75 \u00b5M (56.50 \u00b1 2.33%). In contrast, treatment with 100 \u00b5M H2O2 led to a significant reduction in MII rate to 32.83 \u00b1 7.64%, compared to all other groups. These findings indicated that exposure to 100 \u00b5M H2O2 for 1 h effectively induces oxidative stress in bovine oocytes and could serve as a standard in vitro model for future studies, investigating antioxidant supplementation during pre-IVM and IVM phases.<\/jats:p>","DOI":"10.3390\/ani15223304","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T09:58:42Z","timestamp":1763459922000},"page":"3304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Optimization of Hydrogen Peroxide Concentrations for Inducing Oxidative Stress in Bovine Oocytes Prior to In Vitro Maturation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3390-8406","authenticated-orcid":false,"given":"Sirawit","family":"Yindeetrakul","sequence":"first","affiliation":[{"name":"Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, Nakhon Pathom 73140, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3079-318X","authenticated-orcid":false,"given":"Supawit","family":"Triwutanon","sequence":"additional","affiliation":[{"name":"Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, Nakhon Pathom 73140, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2332-6559","authenticated-orcid":false,"given":"Anawat","family":"Sangmalee","sequence":"additional","affiliation":[{"name":"Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, Nakhon Pathom 73140, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7659-9644","authenticated-orcid":false,"given":"Theera","family":"Rukkwamsuk","sequence":"additional","affiliation":[{"name":"Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, Nakhon Pathom 73140, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"968","DOI":"10.5713\/ajas.18.0201","article-title":"Current situation and future prospects for beef production in Thailand\u2014A review","volume":"31","author":"Bunmee","year":"2018","journal-title":"Asian-Australas. 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