{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:55:39Z","timestamp":1763729739371,"version":"3.45.0"},"reference-count":54,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cOpen Access Publication Fund\u201d of the University of Veterinary Medicine Hannover, Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Spectral detector computed tomography (SDCT) is an innovative imaging technique in veterinary medicine that utilises simultaneous data acquisition at different energy levels using two rows of detectors. This technique provides several interesting applications which improve insights into tissue composition. One implementation is the generation of virtual non-contrast (VNC) images from post-contrast spectral CT data by identifying and subtracting iodine pixels. Preliminary studies suggest that VNC images may offer diagnostic quality comparable to true unenhanced (TUE) images in healthy dogs; however, this technique has yet to be evaluated in clinical patients. This study compared the Hounsfield units (HUs) measured in VNC to those in TUE images of canine hepatic pathologies, taking into account specific types of pathologies based on their imaging characteristics. The attenuation values of the VNC and TUE series were analysed using two one-sided t-tests (TOST), and the signal-to-noise ratio (SNR) was calculated for each region of interest (ROI). A 5-point Likert scale was utilised to assess image noise, quality, and iodine subtraction in the VNC images. A total of 287 ROIs were analysed in the liver, gallbladder, paravertebral muscle, and pancreatic body of 28 dogs with histopathologically confirmed hepatic pathologies. 92.61% of the hepatic ROIs displayed a \u201cnegligible\u201d difference of \u226410 HUs between VNC and TUE images, with significant p-values of &lt;0.05 maintained for all ROIs within the limit of \u226410 HUs in the TOST, confirming equivalence between the two imaging modalities. The image quality assessment indicated that SDCT-derived images provided equal or superior quality compared to conventional CT. Therefore, it can be concluded that VNC images calculated from SDCT data could be an alternative to conventional TUE images for hepatic pathologies.<\/jats:p>","DOI":"10.3390\/ani15233366","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:41:58Z","timestamp":1763728918000},"page":"3366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Virtual Non-Contrast Images Generated by Spectral Detector Computed Tomography and Conventional Computed Tomography Images of Histologically Confirmed Hepatic Pathologies in 28 Dogs"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4550-0748","authenticated-orcid":false,"given":"Lydia K.","family":"Clau\u00dfen","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5518-7748","authenticated-orcid":false,"given":"Alkje M.","family":"van Gemmeren","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Lietz","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Meller","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano","family":"Wang-Leandro","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Beineke","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Nerschbach","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-638X","authenticated-orcid":false,"given":"Holger A.","family":"Volk","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9386-3096","authenticated-orcid":false,"given":"Kristina","family":"Merhof","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1111\/j.1740-8261.2011.01917.x","article-title":"CT characteristics of primary hepatic mass lesions in dogs","volume":"53","author":"Fukushima","year":"2012","journal-title":"Vet. Radiol. Ultrasound"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Stehl\u00edk, L., Di Tommaso, M., Del Signore, F., Panin\u00e1rov\u00e1, M., Terragni, R., Magni, T., Pontonutti, L., Carloni, A., Alberti, M., and De Magistris, A.V. (2020). Triple-Phase Multidetector Computed Tomography in Distinguishing Canine Hepatic Lesions. Animals, 11.","DOI":"10.3390\/ani11010011"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1292\/jvms.19-0278","article-title":"Computed tomographic features for differentiating benign from malignant liver lesions in dogs","volume":"81","author":"Ohta","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1111\/jsap.12921","article-title":"Determining the anatomical origin of canine hepatic masses by CT","volume":"59","author":"Lamb","year":"2018","journal-title":"J. Small Anim. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Burti, S., Zotti, A., Bonsembiante, F., Contiero, B., and Banzato, T. (2021). Diagnostic Accuracy of Delayed Phase Post Contrast Computed Tomographic Images in the Diagnosis of Focal Liver Lesions in Dogs: 69 Cases. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.611556"},{"key":"ref_6","first-page":"17","article-title":"Differential diagnosis of hepatic tumor-like lesions in dog by using dynamic CT scanning","volume":"58","author":"Taniura","year":"2009","journal-title":"Hiroshima J. Med. Sci."},{"key":"ref_7","first-page":"46","article-title":"Comparative evaluation of the liver in dogs with a splenic mass by using ultrasonography and contrast-enhanced computed tomography","volume":"49","author":"Irausquin","year":"2008","journal-title":"Can. Vet. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e1083","DOI":"10.1002\/vetr.1083","article-title":"Comparing the CT and MRI findings for canine primary hepatocellular lesions","volume":"190","author":"Kurokawa","year":"2022","journal-title":"Vet. Rec."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.2460\/ajvr.78.11.1273","article-title":"Evaluation of canine hepatic masses by use of triphasic computed tomography and B-mode, color flow, power, and pulsed-wave Doppler ultrasonography and correlation with histopathologic classification","volume":"78","author":"Griebie","year":"2017","journal-title":"Am. J. Vet. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/BF00327254","article-title":"X-ray energies for effective atomic number determination","volume":"11","author":"Rutherford","year":"1976","journal-title":"Neuroradiology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1118\/1.594555","article-title":"Extraction of information from CT scans at different energies","volume":"6","author":"Millner","year":"1979","journal-title":"Med. Phys."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.2214\/AJR.12.9196","article-title":"Dual-energy CT applications in the abdomen","volume":"199","author":"Heye","year":"2012","journal-title":"AJR Am. J. Roentgenol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1148\/rg.304095175","article-title":"Dual-energy multidetector CT: How does it work, what can it tell us, and when can we use it in abdominopelvic imaging?","volume":"30","author":"Coursey","year":"2010","journal-title":"Radiographics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1007\/s13244-017-0571-4","article-title":"Detector-based spectral CT with a novel dual-layer technology: Principles and applications","volume":"8","author":"Rassouli","year":"2017","journal-title":"Insights Imaging"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.ejrad.2018.05.007","article-title":"Dual-layer spectral computed tomography: Virtual non-contrast in comparison to true non-contrast images","volume":"104","author":"Sauter","year":"2018","journal-title":"Eur. J. Radiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.diii.2015.11.018","article-title":"Applications of dual energy computed tomography in abdominal imaging","volume":"97","author":"Lestra","year":"2016","journal-title":"Diagn. Interv. Imaging"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.ejrad.2014.07.009","article-title":"Quantitative analysis of the dual-energy CT virtual spectral curve for focal liver lesions characterization","volume":"83","author":"Wang","year":"2014","journal-title":"Eur. J. Radiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6994","DOI":"10.1038\/s41598-020-64002-7","article-title":"Iodine accumulation of the liver in patients treated with amiodarone can be unmasked using material decomposition from multiphase spectral-detector CT","volume":"10","author":"Laukamp","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_19","first-page":"1429","article-title":"Clinical value of spectral CT imaging combined with AFP in identifying liver cancer and hepatic focal nodular hyperplasia","volume":"24","author":"Xu","year":"2019","journal-title":"J. Buon"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3348\/kjr.2012.13.4.434","article-title":"Spectral CT: Preliminary studies in the liver cirrhosis","volume":"13","author":"Lv","year":"2012","journal-title":"Korean J. Radiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00330-008-1122-7","article-title":"Dual energy CT: Preliminary observations and potential clinical applications in the abdomen","volume":"19","author":"Graser","year":"2009","journal-title":"Eur. Radiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1177\/0284185119893094","article-title":"Virtual non-contrast for evaluation of liver parenchyma and vessels: Results from 25 patients using multi-phase spectral-detector CT","volume":"61","author":"Laukamp","year":"2020","journal-title":"Acta Radiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.acra.2013.01.010","article-title":"Tin-filter enhanced dual-energy-CT: Image quality and accuracy of CT numbers in virtual noncontrast imaging","volume":"20","author":"Kaufmann","year":"2013","journal-title":"Acad. Radiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.diii.2019.04.007","article-title":"Virtual unenhanced phase with spectral dual-energy CT: Is it an alternative to conventional true unenhanced phase for abdominal tissues?","volume":"100","author":"Jamali","year":"2019","journal-title":"Diagn. Interv. Imaging"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1007\/s00261-016-1036-9","article-title":"Spectral detector CT-derived virtual non-contrast images: Comparison of attenuation values with unenhanced CT","volume":"42","author":"Ananthakrishnan","year":"2017","journal-title":"Abdom. Radiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yang, P., Zhao, R., Deng, W., An, S., Li, Y., Sheng, M., Chen, X., Qian, Y., Yu, Y., and Mu, D. (2023). Feasibility and accuracy of coronary artery calcium score on virtual non-contrast images derived from a dual-layer spectral detector CT: A retrospective multicenter study. Front. Cardiovasc. Med., 10.","DOI":"10.3389\/fcvm.2023.1114058"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bucolo, G.M., Ascenti, V., Barbera, S., Fontana, F., Aric\u00f2, F.M., Piacentino, F., Coppola, A., Cicero, G., Marino, M.A., and Booz, C. (2023). Virtual Non-Contrast Spectral CT in Renal Masses: Is It Time to Discard Conventional Unenhanced Phase?. J. Clin. Med., 12.","DOI":"10.3390\/jcm12144718"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s13244-021-01146-8","article-title":"Utilisation of virtual non-contrast images and virtual mono-energetic images acquired from dual-layer spectral CT for renal cell carcinoma: Image quality and radiation dose","volume":"13","author":"Zhang","year":"2022","journal-title":"Insights Imaging"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.7314\/APJCP.2015.16.6.2521","article-title":"Virtual non-contrast computer tomography (CT) with spectral CT as an alternative to conventional unenhanced CT in the assessment of gastric cancer","volume":"16","author":"Tian","year":"2015","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Steinhardt, M., Marka, A.W., Ziegelmayer, S., Makowski, M., Braren, R., Graf, M., and Gawlitza, J. (2024). Comparison of Virtual Non-Contrast and True Non-Contrast CT Images Obtained by Dual-Layer Spectral CT in COPD Patients. Bioengineering, 11.","DOI":"10.3390\/bioengineering11040301"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"20220157","DOI":"10.1259\/bjr.20220157","article-title":"Comparison of true non-contrast and virtual non-contrast images in the characterization of renal lesions using detector-based spectral CT","volume":"96","author":"Verstraeten","year":"2023","journal-title":"Br. J. Radiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6762","DOI":"10.1007\/s00330-019-06322-5","article-title":"Virtual versus true non-contrast dual-energy CT imaging for the diagnosis of aortic intramural hematoma","volume":"29","author":"Dupuis","year":"2019","journal-title":"Eur. Radiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4324","DOI":"10.1007\/s00330-017-4785-0","article-title":"Diagnostic accuracy of virtual non-contrast enhanced dual-energy CT for diagnosis of adrenal adenoma: A systematic review and meta-analysis","volume":"27","author":"Connolly","year":"2017","journal-title":"Eur. Radiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"81.e21","DOI":"10.1016\/j.crad.2020.08.026","article-title":"Replacing true unenhanced imaging in renal carcinoma with virtual unenhanced images in dual-energy spectral CT: A feasibility study","volume":"76","author":"Ma","year":"2021","journal-title":"Clin. Radiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Lietz, P., Br\u00fcntgens, M., Wang-Leandro, A., Volk, H.A., Meller, S., and Merhof, K. (2023). Virtual non-contrast images of detector-based spectral computed tomography in dogs: A promising alternative to true non-contrast images in veterinary medicine. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1251535"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"van Gemmeren, A.M., Clau\u00dfen, L.K., Lietz, P., Meller, S., Wang-Leandro, A., Beineke, A., Nerschbach, V., Volk, H.A., and Merhof, K. (2025). Spectral detector computed tomography imaging of histologically confirmed splenic pathologies in 30 canine patients: A comparison of virtual non-contrast images and true unenhanced images. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1645439"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Miki\u0107, M., Lietz, P., Dierig, J.A., Meller, S., Pees, M., and Merhof, K. (2025). Evaluation of virtual non-contrast detector-based spectral CT images in comparison to true unenhanced images in 20 rabbits. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1521986"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Szablics, F., B\u00e9rczi, \u00c1., Cs\u0151re, J., Borzs\u00e1k, S., Szentiv\u00e1nyi, A., Kiss, M., Juh\u00e1sz, G., Papp, D., Suhai, F.I., and Csobay-Nov\u00e1k, C. (2025). Virtual Non-Contrast Reconstructions Derived from Dual-Energy CTA Scans in Peripheral Arterial Disease: Comparison with True Non-Contrast Images and Impact on Radiation Dose. J. Clin. Med., 14.","DOI":"10.3390\/jcm14155571"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1097\/RCT.0000000000001450","article-title":"Optimal Virtual Monoenergetic Photon Energy (keV) for Photon-Counting-Detector Computed Tomography Angiography","volume":"47","author":"Dunning","year":"2023","journal-title":"J. Comput. Assist. Tomogr."},{"key":"ref_40","first-page":"20180546","article-title":"Dual energy computed tomography virtual monoenergetic imaging: Technique and clinical applications","volume":"92","author":"Cicero","year":"2019","journal-title":"Br. J. Radiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1007\/s11547-024-01835-6","article-title":"Optimization of window settings for coronary arteries assessment using spectral CT-derived virtual monoenergetic imaging","volume":"129","author":"Mastrodicasa","year":"2024","journal-title":"Radiol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1007\/s00261-017-1151-2","article-title":"Assessment of 70-keV virtual monoenergetic spectral images in abdominal CT imaging: A comparison study to conventional polychromatic 120-kVp images","volume":"42","author":"Rassouli","year":"2017","journal-title":"Abdom. Radiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e22955","DOI":"10.1002\/ima.22955","article-title":"Stacked deep model-based classification of the multiclass brain hemorrhages in CT scans","volume":"34","author":"Malik","year":"2024","journal-title":"Int. J. Imaging Syst. Technol."},{"key":"ref_44","unstructured":"Rothuizen, J., Bunch, S.E., Charles, J.A., Cullen, J.M., Desmet, V.J., Szatm\u00e1ri, V., Twedt, D.C., van den Ingh, T.S., van Winkle, T., and Washabau, R.J. (2006). WSAVA Standards for Clinical and Histological Diagnosis of Canine and Feline Liver Diseases, Saunders Limited."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1007\/s00330-020-07298-3","article-title":"Quantitative distribution of iodinated contrast media in body computed tomography: Data from a large reference cohort","volume":"31","author":"Zopfs","year":"2021","journal-title":"Eur. Radiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.2214\/AJR.17.18248","article-title":"Abdominal Attenuation Values on Virtual and True Unenhanced Images Obtained With Third-Generation Dual-Source Dual-Energy CT","volume":"210","author":"Durieux","year":"2018","journal-title":"AJR Am. J. Roentgenol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3348\/kjr.2020.0144","article-title":"Spectral Computed Tomography: Fundamental Principles and Recent Developments","volume":"22","author":"So","year":"2021","journal-title":"Korean J. Radiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1148\/radiol.12110851","article-title":"Virtual nonenhanced dual-energy CT urography with tin-filter technology: Determinants of detection of urinary calculi in the renal collecting system","volume":"264","author":"Mangold","year":"2012","journal-title":"Radiology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6260","DOI":"10.21037\/qims-24-535","article-title":"Comparison of virtual and true non-contrast images from dual-layer spectral detector computed tomography (CT) in patients with colorectal cancer","volume":"14","author":"Wen","year":"2024","journal-title":"Quant. Imaging Med. Surg."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1097\/RCT.0000000000000903","article-title":"Comparing Arterial- and Venous-Phase Acquisition for Optimization of Virtual Noncontrast Images From Dual-Energy Computed Tomography Angiography","volume":"43","author":"Lehti","year":"2019","journal-title":"J. Comput. Assist. Tomogr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1097\/RCT.0000000000000982","article-title":"Virtual Noncontrast Images From Portal Venous Phase Spectral-Detector CT Acquisitions for Adrenal Lesion Characterization","volume":"45","author":"Laukamp","year":"2021","journal-title":"J. Comput. Assist. Tomogr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"108740","DOI":"10.1016\/j.ejrad.2019.108740","article-title":"Association between true non-contrast and virtual non-contrast vertebral bone CT attenuation values determined using dual-layer spectral detector CT","volume":"121","author":"Ding","year":"2019","journal-title":"Eur. J. Radiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2752","DOI":"10.1007\/s00261-017-1170-z","article-title":"Quality of routine diagnostic abdominal images generated from a novel detector-based spectral CT scanner: A technical report on a phantom and clinical study","volume":"42","author":"Hojjati","year":"2017","journal-title":"Abdom. Radiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.diii.2022.11.003","article-title":"Spectral CT imaging: Technical principles of dual-energy CT and multi-energy photon-counting CT","volume":"104","author":"Greffier","year":"2023","journal-title":"Diagn. Interv. Imaging"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/23\/3366\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:51:20Z","timestamp":1763729480000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/23\/3366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,21]]},"references-count":54,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ani15233366"],"URL":"https:\/\/doi.org\/10.3390\/ani15233366","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,21]]}}}