{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:21:52Z","timestamp":1790281312033,"version":"4.1.0"},"reference-count":82,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Shaanxi Livestock and Poultry Breeding Double-chain Fusion Key Project","award":["2022GD-TSLD-46-0501"],"award-info":[{"award-number":["2022GD-TSLD-46-0501"]}]},{"name":"the Agricultural Special Funds of Shaanxi Province, China"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study aimed to investigate the effects of rumen-protected methionine (RPM) on the production performance of lactating dairy goats. Thirty first-time lactating Guanzhong dairy goats with identical kidding dates and comparable body weights (41.17 \u00b1 3.05 kg) were randomly assigned to two groups: (1) CON: basal diet and (2) RPM: basal diet + 7.5 g\/day RPM. The duration of the experiment was 21 days. Compared with the CON group, the RPM group presented a significant increase in milk yield, 4% fat-corrected milk (FCM), and feed efficiency; however, no significant difference was observed in dry feed intake (DMI). Moreover, milk fat, protein, lactose, and SNF production was greater in the PRM group than in the CON group. Compared with the CON group, the RPM group presented higher nonesterified fatty acid (NEFA) and very-low-density lipoprotein (VLDL) levels, and no significant differences in the other metabolites were detected. The concentrations of acetate, propionate, and total volatile fatty acids (TVFAs) in the feces of the RPM group were significantly greater than those in the CON group; however, no significant differences were detected in the concentrations of isobutyrate, butyrate, and valerate. Furthermore, genera such as Muribaculaceae, Bifidobacterium, and Christensenellaceae were significantly enriched in the feces of the RPM group. Concurrently, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the RPM group was significantly enriched in pathways associated with amino acid synthesis, the immune system, and energy metabolism. In summary, dietary supplementation with RPM improved the lipid metabolism function of the liver, increased the abundance of beneficial bacteria such as Muribaculaceae and Bifidobacterium in the colon, and enriched microbial functions related to energy and amino acid metabolism, thereby enhancing colon fermentation and host metabolic status, ultimately improving the production performance of lactating dairy goats. These findings elucidate the positive effects of RPM on the production performance and metabolic health of dairy goats, potentially offering new perspectives and strategies for optimizing dairy production.<\/jats:p>","DOI":"10.3390\/ani15233386","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T11:47:22Z","timestamp":1763984842000},"page":"3386","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rumen-Protected Methionine Supplementation in the Diet Improved the Production Performance of Dairy Goats by Optimizing the Amino Acid Profile and Lipid Metabolism and Modulating the Colonic Microbiome"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1870-640X","authenticated-orcid":false,"given":"Xingwei","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1046-3477","authenticated-orcid":false,"given":"Shengru","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhu","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E105","DOI":"10.3168\/jds.S0022-0302(04)70066-1","article-title":"Nutritional Management of Transition Dairy Cows: Strategies to Optimize Metabolic Health","volume":"87","author":"Overton","year":"2004","journal-title":"J. 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