{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T12:31:01Z","timestamp":1782131461033,"version":"3.54.5"},"reference-count":70,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Kittens are born with some maternally derived antibodies (MDA) that, together with maternal gut microbiota, bring some form of early defense against pathogens. However, this protection declines and the rapid changes and challenges the kitten typically encounters can adversely affect their health. Nutrition can modulate the immune system and gut microbiota, offering a boost to protection following weaning. This controlled, randomized, double-blinded study tests a dietary supplement containing nucleotides, oligosaccharides, vitamin E and \u03b2-carotene in 50 domestic short-haired kittens. Control kittens were fed standard, dry kitten diet whilst test kittens received the same diet with supplement for 52 weeks. Kittens received routine vaccinations and blood samples were collected throughout the study for standard hematology, serum antibodies and cytokine expression. Rectal fecal samples were analyzed for gut microbiota via 16S rRNA gene sequencing. Supplemented kittens showed significantly improved antibody response to Chlamydia vaccine at various timepoints versus controls, with no consistent effects of supplementation on responses to other vaccines. Several cytokines were significantly influenced by the supplement, which also significantly altered gut microbiota diversity. In conclusion, a dietary supplement was shown to promote immune response and gut microbiota changes in kittens and may help to support a healthy transition into adulthood.<\/jats:p>","DOI":"10.3390\/ani15233504","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T10:50:38Z","timestamp":1764931838000},"page":"3504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Dietary Supplement Containing Nucleotides, Oligosaccharides, Vitamin E and \u03b2-Carotene Promotes Immune Response and Gut Microbiota Changes in Kittens"],"prefix":"10.3390","volume":"15","author":[{"given":"Willy","family":"Joly","sequence":"first","affiliation":[{"name":"Royal Canin Research Center, 650 Avenue de la Petite Camargue, 30470 Aimargues, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1063-7768","authenticated-orcid":false,"given":"Matthew","family":"Harrison","sequence":"additional","affiliation":[{"name":"Waltham Petcare Science Institute, Mars Petcare, Waltham on the Wolds, Melton Mowbray, Leicestershire LE14 4RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Laxalde","sequence":"additional","affiliation":[{"name":"Royal Canin Research Center, 650 Avenue de la Petite Camargue, 30470 Aimargues, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4219-5905","authenticated-orcid":false,"given":"Virginie","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Royal Canin Research Center, 650 Avenue de la Petite Camargue, 30470 Aimargues, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Maggini, S., Pierre, A., and Calder, P.C. (2018). Immune Function and Micronutrient Requirements Change over the Life Course. Nutrients, 10.","DOI":"10.3390\/nu10101531"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1079\/095442200108728981","article-title":"Undernutrition, Infection and Immune Function","volume":"13","author":"Calder","year":"2000","journal-title":"Nutr. Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Deusch, O., O\u2019Flynn, C., Colyer, A., Morris, P., Allaway, D., Jones, P.G., and Swanson, K.S. (2014). Deep Illumina-based Shotgun Sequencing Reveals Dietary Effects on the Structure and Function of the Fecal Microbiome of Growing Kittens. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101021"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Munteanu, C., and Schwartz, B. (2022). The Relationship Between Nutrition and the Immune System. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1082500"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Childs, C.E., Calder, P.C., and Miles, E.A. (2019). Diet and Immune Function. Nutrients, 11.","DOI":"10.3390\/nu11081933"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1177\/0884533611434933","article-title":"The Role of Nucleotides in the Immune and Gastrointestinal Systems: Potential Clinical Applications","volume":"27","author":"Hess","year":"2012","journal-title":"Nutr. Clin. Pract."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1080\/1745039X.2010.496952","article-title":"Nucleotides in Canine Colostrum and Milk at Different Stages of Lactation","volume":"64","author":"Tonini","year":"2010","journal-title":"Arch. Anim. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1439-0396.2007.00680_3.x","article-title":"Dietary Nucleotides Improve the Immune Status of Puppies at Weaning","volume":"91","author":"Romano","year":"2007","journal-title":"J. Anim. Phys. Anim. Nutr."},{"key":"ref_9","first-page":"453","article-title":"Dietary nucleotides accelerate changes in intestinal lymphocyte maturation in weanling mice","volume":"37","author":"Manzano","year":"2003","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.2527\/jas.2009-2464","article-title":"Dietary Cellulose, Fructooligosaccharides, and Pectin Modify Fecal Protein Catabolites and Microbial Populations in Adult Cats","volume":"88","author":"Barry","year":"2010","journal-title":"J. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/s41575-019-0173-3","article-title":"Probiotics and Prebiotics in Intestinal Health and Disease: From Biology to the Clinic","volume":"16","author":"Sanders","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1439-0396.2007.00688.x","article-title":"Effects of Dietary scFOS on Immunoglobulins in Colostrums and Milk of Bitches","volume":"91","author":"Adogony","year":"2007","journal-title":"J. Anim. Phys. Anim. Nutr."},{"key":"ref_13","first-page":"131","article-title":"Xylooligosaccharides: An Economical Prebiotic From Agroresidues and Their Health Benefits","volume":"53","author":"Jain","year":"2015","journal-title":"Indian J. Exp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Christensen, E.G., Licht, T.R., Leser, T.D., and Bahl, M.I. (2014). Dietary Xylo-oligosaccharide Stimulates Intestinal Bifidobacteria and Lactobacilli but has Limited Effect on Intestinal Integrity in Rats. BMC Res. Notes, 7.","DOI":"10.1186\/1756-0500-7-660"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1017\/S0007114513004261","article-title":"Xylo-oligosaccharides Alone or in Synbiotic Combination with Bifidobacterium animalis subsp. lactis Induce Bifidogenesis and Modulate Markers of Immune Function in Healthy Adults: A Double-Blind, Placebo-Controlled, Randomised, Factorial Cross-Over Study","volume":"111","author":"Childs","year":"2014","journal-title":"Brit J. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.carres.2007.02.017","article-title":"Almond Shell Xylo-Oligosaccharides Exhibiting Immunostimulatory Activity","volume":"342","author":"Nabarlatz","year":"2007","journal-title":"Carbohydr Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3136\/fstr.18.195","article-title":"Immunomodulatory Effects of Xylooligosaccharides","volume":"18","author":"Chen","year":"2012","journal-title":"Food Sci. Technol. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.rvsc.2015.01.007","article-title":"Moderate Dietary Supplementation with Vitamin E Enhances Lymphocyte Functionality in the Adult Cat","volume":"99","author":"Thomas","year":"2015","journal-title":"Res. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1610S","DOI":"10.1093\/jn\/132.6.1610S","article-title":"Cats Absorb \u03b2-Carotene, but It Is Not Converted to Vitamin A","volume":"132","author":"Schweigert","year":"2002","journal-title":"J. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1093\/jn\/130.8.1910","article-title":"Dietary Beta-Carotene Stimulates Cell-Mediated and Humoral Immune Response in Dogs","volume":"130","author":"Chew","year":"2000","journal-title":"J. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Atwal, J., Joly, W., Bednall, R., Albanese, F., Farquhar, M., Holcombe, L.J., Watson, P., and Harrison, M. (2023). Dietary Supplementation with Nucleotides, Short-Chain Fructooligosaccharides, Xylooligosaccharides, Beta-Carotene and Vitamin E Influences Immune Function in Kittens. Animals, 13.","DOI":"10.3390\/ani13233734"},{"key":"ref_22","unstructured":"National Research Council (2006). Nutrient Requirements of Dogs and Cats."},{"key":"ref_23","first-page":"111","article-title":"Finding Alternatives: An Overview of the 3Rs and the Use of Animals in Research","volume":"87","author":"Robinson","year":"2005","journal-title":"School Sci. Rev."},{"key":"ref_24","first-page":"1","article-title":"The ARRIVE Guidelines 2.0: Updated Guidelines for Reporting Animal Research","volume":"18","author":"Sert","year":"2020","journal-title":"PLoS Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME Improves Sensitivity and Speed of Chimera Detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2584","DOI":"10.7717\/peerj.2584","article-title":"VSEARCH: A Versatile Open Source Tool for Metagenomics","volume":"4","author":"Rognes","year":"2016","journal-title":"Peer J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1038\/ismej.2014.195","article-title":"Minimum Entropy Decomposition: Unsupervised Oligotyping for Sensitive Partitioning of High-Throughput Marker Gene Sequences","volume":"9","author":"Eren","year":"2015","journal-title":"ISME J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1111\/2041-210X.12114","article-title":"Oligotyping: Differentiating Between Closely Related Microbial Taxa Using 16S rRNA Gene Data","volume":"4","author":"Eren","year":"2013","journal-title":"Methods Ecol. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_30","unstructured":"R Core Team (2021). R: A Language and Environment for Statistical Computing, version 4.1.0, R Foundation for Statistical Computing."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). Ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_32","unstructured":"Wickham, H., Fran\u00e7ois, R., Henry, L., M\u00fcller, K., and Vaughan, D. (2025, December 01). dplyr: A Grammar of Data Manipulation. R Package, Available online: https:\/\/dplyr.tidyverse.org."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting Linear Mixed-Effects Models Using lme4","volume":"67","author":"Bates","year":"2015","journal-title":"J. Stat. Software"},{"key":"ref_34","unstructured":"Lenth, R. (R Package, 2022). emmeans: Estimated Marginal Means, aka Least-Squares Means, R Package, Version 1.8.2."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/j.1461-0248.2006.00926.x","article-title":"Multivariate dispersion as a measure of beta diversity","volume":"9","author":"Anderson","year":"2006","journal-title":"Ecol. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated Estimation of Fold Change and Dispersion for RNA-seq Data with DESeq2. Gen. Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Salt, C., Butterwick, R.F., Henzel, K.S., and German, A.J. (2023). Comparison of Growth in Neutered Domestic Shorthair kittens With Growth in Sexually-Intact Cats. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0283016"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1017\/S0007114511001851","article-title":"Effects of Neutering on Food Intake, Body Weight and Body Composition in Growing Female Kittens","volume":"106","author":"Alexander","year":"2011","journal-title":"Brit. J. Nutr."},{"key":"ref_39","first-page":"811","article-title":"Normal Hematology of the Cat","volume":"Volume 105","author":"Weiss","year":"2011","journal-title":"Schalm\u2019s Veterinary Hematology"},{"key":"ref_40","first-page":"365","article-title":"Generating and Interpreting Test Results: Test Validity, Quality Control, Reference Values and Basic Epidemiology","volume":"Volume 13","author":"Latimer","year":"2011","journal-title":"Duncan & Prasse\u2019s Veterinary Laboratory Medicine: Clinical Pathology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1038\/sj.ejcn.1601475","article-title":"Modulation of the Immune Response Mediated by Dietary Nucleotides","volume":"56","author":"Gil","year":"2002","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"17","DOI":"10.17221\/35\/2020-VETMED","article-title":"Effects of Dietary Nucleotides and Cationic Peptides on Vaccination Response in Cats","volume":"66","author":"Vojtek","year":"2021","journal-title":"Vet. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.it.2006.11.002","article-title":"IL-12p40: An Inherently Agonistic Cytokine","volume":"28","author":"Cooper","year":"2007","journal-title":"Trends Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1556\/030.62.2015.4.2","article-title":"Interleukin-13 as an important cytokine: A review on its roles in some human diseases","volume":"62","author":"Seyfizadeh","year":"2015","journal-title":"Acta Microbiol. Immunol. Hung."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0167-5699(94)90021-3","article-title":"Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells","volume":"15","author":"Zurawski","year":"1994","journal-title":"Immunol. Today"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"19589","DOI":"10.1038\/s41598-023-46237-2","article-title":"IL-4 and IL-13 induce equivalent expression of traditional M2 markers and modulation of reactive oxygen species in human macrophages","volume":"13","author":"Scott","year":"2023","journal-title":"Sci Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"109130","DOI":"10.1016\/j.intimp.2022.109130","article-title":"Interleukin-6 Cytokine: An Overview of the Immune Regulation, Immune Dysregulation, and Therapeutic Approach","volume":"111","author":"Aliyu","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1046\/j.1365-3164.2003.00353.x","article-title":"Expression of RANTES mRNA in Skin Lesions of Feline Eosinophilic Plaque","volume":"14","author":"Kimura","year":"2003","journal-title":"Vet. Dermatol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Hill, C.J., Lynch, D.B., Murphy, K., Ulaszewska, M., Jeffery, I.B., O\u2019Shea, C.A., Watkins, C., Dempsey, E., Mattivi, F., and Tuohy, K. (2017). Evolution of Gut Microbiota Composition from Birth to 24 weeks in the INFANTMET Cohort. Microbiome, 5.","DOI":"10.1186\/s40168-016-0213-y"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4314\/ovj.v9i3.10","article-title":"Role of Gut Microbiota in Dog and Cat\u2019s Health and Diseases","volume":"9","author":"Mondo","year":"2019","journal-title":"Open Vet. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"101186","DOI":"10.1016\/j.onehlt.2025.101186","article-title":"Gut Microbiome Disruption in Free-Roaming Cats: Do Antibiotics Reduce or Restructure Zoonotic Risk?","volume":"21","author":"Xie","year":"2025","journal-title":"One Health"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Lyu, Y., Su, C., Verbrugghe, A., Van de Wiele, T., Martos Martinez-Caja, A., and Hesta, M. (2020). Past, Present, and Future of Gastrointestinal Microbiota Research in Cats. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01661"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Ganz, H.H., Jospin, G., Rojas, C.A., Martin, A.L., Dahlhausen, K., Kingsbury, D.D., Osborne, C.X., Entrolezo, Z., Redner, S., and Ramirez, B. (2022). The Kitty Microbiome Project: Defining the Healthy Fecal \u201cCore Microbiome\u201d in Pet Domestic Cats. Vet. Sci., 9.","DOI":"10.3390\/vetsci9110635"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s42523-024-00373-w","article-title":"Dynamic Development of Gut Microbiota and Metabolism During and After Weaning of Kittens","volume":"7","author":"Zhang","year":"2025","journal-title":"Anim. Microb."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1017\/S0007114514002943","article-title":"Gut Microbiota of Humans, Dogs and Cats: Current Knowledge and Future Opportunities and Challenges","volume":"13","author":"Deng","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Li, Z., Di, D., Sun, Q., Yao, X., Wei, J., Li, B., Liu, K., Shao, D., Qiu, Y., and Liu, H. (2022). Comparative Analyses of the Gut Microbiota in Growing Ragdoll Cats and Felinae Cats. Animals, 12.","DOI":"10.3390\/ani12182467"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.nmni.2016.11.002","article-title":"\u201cNegativibacillus massiliensis\u201d gen. nov., sp. nov., Isolated From Human Left Colon","volume":"17","author":"Ricaboni","year":"2017","journal-title":"New Microb. New Infect."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00203-017-1463-1","article-title":"Glucerabacter canisensis gen. nov., sp. nov., Isolated From Dog Feces and its Effect on the Hydrolysis of Plant Glucosylceramide in the Intestine of Dogs","volume":"200","author":"Kawata","year":"2018","journal-title":"Arch. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Cunha, F., Zhai, Y., Casaro, S., Jones, K.L., Hernandez, M., Bisinotto, R.S., Kariyawasam, S., Brown, M.B., Phillips, A., and Jeong, K.C. (2024). Pangenomic and Biochemical Analyses of Helcococcus ovis Reveal Widespread Tetracycline Resistance and a Novel Bacterial Species, Helcococcus bovis. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1456569"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1111\/1574-6976.12005","article-title":"Gram-Positive Anaerobic Cocci-Commensals and Opportunistic Pathogens","volume":"37","author":"Murphy","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Rojas, C.A., Marks, S.L., Borras, E., Lesea, H., McCartney, M.M., Coil, D.A., Davis, C.E., and Eisen, J.A. (2023). Characterization of the Microbiome and Volatile Compounds in Anal Gland Secretions from Domestic Cats (Felis catus) Using Metagenomics and Metabolomics. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-45997-1"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.2527\/jas.2010-3201","article-title":"Effects of Short-Chain Fructooligosaccharides and Galactooligosaccharides, Individually and in Combination, on Nutrient Digestibility, Fecal Fermentative Metabolite Concentrations, and Large Bowel Microbial Ecology of Healthy Adult Cats","volume":"89","author":"Kanakupt","year":"2011","journal-title":"J. Anim. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"436","DOI":"10.2460\/ajvr.1998.59.04.436","article-title":"Effect of Dietary Supplementation with Fructo-oligosaccharides on Fecal Flora of Healthy Cats","volume":"59","author":"Sparkes","year":"1998","journal-title":"Am. J. Vet. Res."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Lyu, Y., Debevere, S., Bourgeois, H., Ran, M., Broeckx, B.J.G., Vanhaecke, L., Wiele, T.V., and Hesta, M. (2020). Dose-Dependent Effects of Dietary Xylooligosaccharides Supplementation on Microbiota, Fermentation and Metabolism in Healthy Adult Cats. Molecules, 25.","DOI":"10.3390\/molecules25215030"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.cvsm.2017.10.008","article-title":"The Microbiota Regulates Immunity and Immunologic Diseases in Dogs and Cats","volume":"48","author":"Tizard","year":"2018","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0377-8401(95)00891-8","article-title":"Importance of Antioxidant Vitamins in Immunity and Health in Animals","volume":"59","author":"Chew","year":"1996","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1017\/S0029665199000932","article-title":"Effects of Carotenoids on Human Immune Function","volume":"58","author":"Hughes","year":"1999","journal-title":"Proc. Nutr. Soc."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Deusch, O., O\u2019Flynn, C., Colyer, A., Swanson, K.S., Allaway, D., and Morris, P.A. (2015). Longitudinal Study of the Feline Faecal Microbiome Identifies Changes into Early Adulthood Irrespective of Sexual Development. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0144881"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1017\/S0007114517002379","article-title":"Effects of Obesity, Energy Restriction and Neutering on the Faecal Microbiota of Cats","volume":"118","author":"Fischer","year":"2017","journal-title":"Br. J. Nutr."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Radhakrishnan, S.T., Gallagher, K.I., Mullish, B.H., Serrano-Contreras, J.I., Alexander, J.L., Miguens Blanco, J., Danckert, N.P., Valdivia-Garcia, M., Hopkins, B.J., and Ghai, A. (2023). Rectal Swabs as a Viable Alternative to Faecal Sampling for the Analysis of Gut Microbiota Functionality and Composition. Sci. Rep., 13.","DOI":"10.1038\/s41598-022-27131-9"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/23\/3504\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:20:52Z","timestamp":1764937252000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/23\/3504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,4]]},"references-count":70,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ani15233504"],"URL":"https:\/\/doi.org\/10.3390\/ani15233504","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,4]]}}}