{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T05:20:38Z","timestamp":1766553638464,"version":"3.48.0"},"reference-count":53,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Hebei Natural Science Foundation","doi-asserted-by":"crossref","award":["C2022204255"],"award-info":[{"award-number":["C2022204255"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003787","name":"Hebei Natural Science Foundation","doi-asserted-by":"crossref","award":["C2022204256"],"award-info":[{"award-number":["C2022204256"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The extensive use of antibiotics in intensive farming weakens immunity and threatens food safety. Stevia isochlorogenic acid (SICA), a kind of dicaffeoylquinic acid derived from stevia residue, exhibits strong antioxidant activity. This study evaluated the ability of SICA to improve immune function in an immunosuppressed broiler model. SICA significantly increased the spleen, thymus, and bursa of Fabricius indices (p &lt; 0.05), alleviated spleen damage, and elevated serum interleukin-2 (IL-2), IL-4, interferon-\u03b3, IL-1\u03b2, tumor necrosis factor-\u03b1, immunoglobulins (IgA, IgM, IgG), and complement components C3 and C4 (p &lt; 0.05). Isobaric tags for relative and absolute quantification-based proteomics indicated that SICA enhanced splenic immune function by activating cell adhesion molecules, phagosomes, and the intestinal immune network for IgA production pathways. Quantitative PCR analysis showed upregulation of mRNA and protein levels of B-cell receptor, major histocompatibility complex class II, protein tyrosine phosphatase receptor type C, and neutrophil cytosolic factor 2 (p67phox) and downregulation of C-C motif chemokine receptor 9. Molecular docking demonstrated the strongest binding affinity between SICA and p67phox. Overall, SICA effectively alleviated immunosuppression in broiler chickens and represents a promising natural alternative to antibiotic feed additives.<\/jats:p>","DOI":"10.3390\/ani16010025","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T08:35:27Z","timestamp":1766392527000},"page":"25","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["iTRAQ-Based Proteomics Reveals the Potential Mechanisms Underlying Diet Supplementation with Stevia Isochlorogenic Acid That Alleviates Immunosuppression in Cyclophosphamide-Treated Broilers"],"prefix":"10.3390","volume":"16","author":[{"given":"Jiatong","family":"Jin","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqi","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chen Guang Biotechnology Group Co., Ltd., Handan 057250, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yushuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifei","family":"Wu","sequence":"additional","affiliation":[{"name":"Chen Guang Biotechnology Group Co., Ltd., Handan 057250, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangfu","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasai","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100444","DOI":"10.1016\/j.afres.2024.100444","article-title":"The impact of gamma irradiation on the quality of meat and poultry: A review on its immediate and storage effects","volume":"4","author":"Hashim","year":"2024","journal-title":"Appl. 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