{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:22:54Z","timestamp":1780521774835,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1301101"],"award-info":[{"award-number":["2022YFD1301101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1300505"],"award-info":[{"award-number":["2022YFD1300505"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The earmarked fund for China Agriculture Research System","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["Y2025YC52"],"award-info":[{"award-number":["Y2025YC52"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ASTIP-IAS07"],"award-info":[{"award-number":["ASTIP-IAS07"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Early-life development of immune functions is crucial for calf health, growth, and future productivity. Galacto-oligosaccharides (GOSs) have been reported to facilitate ruminal microbial establishment and improve growth in Holstein dairy calves, but their prolonged influence on immunoglobulin levels, hindgut microbiota, and metabolic regulation remains insufficiently understood. This study evaluated the effects of early-life GOS supplementation on immune-related indicators, intestinal microbial ecology, and metabolic profiles in Holstein calves. Twenty-four newborn Holstein female dairy calves were randomly assigned to a control group (CON, n = 12) or a GOS group (GOS, n = 12; 10 g\/day from birth to day 28). After supplementation ceased on day 28, calves previously receiving GOS were referred to as the GOSS group (n = 6). Immunoglobulin levels, gut microbiota, and fecal and serum metabolomes were evaluated during supplementation and six weeks after withdrawal. GOS supplementation significantly increased serum IgA and IgG levels during the treatment, with IgG levels remaining elevated for six weeks after discontinued supplementation. Although overall microbial diversity was not markedly altered, GOS selectively enriched bacterial taxa and function pathways linked to amino acid synthesis, unsaturated fatty acid production, and coenzyme-related metabolism. On day 70, GOSS group displayed distinct fecal and serum metabolomic profiles, with altered metabolites primarily associated with vitamin B6, folate, cobalamin metabolism, branched-chain amino acid biosynthesis, and purine and arginine pathways. These results demonstrate that early-life GOS supplementation promotes sustained immune and metabolic alterations following supplementation cessation, potentially mediated by modulation of gut microbial functions. These findings suggest that early dietary GOS supplementation may support physiological maturation in calves and could be useful as a nutritional strategy in calf-rearing systems.<\/jats:p>","DOI":"10.3390\/ani16010126","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T09:18:56Z","timestamp":1767345536000},"page":"126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Early-Life Galacto-Oligosaccharide Supplementation Induces Persistent Immunoglobulin and Metabolic Alterations in Holstein Dairy Calves by Shaping Gut Microbiota"],"prefix":"10.3390","volume":"16","author":[{"given":"Qi","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meinan","family":"Chang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-265X","authenticated-orcid":false,"given":"Peng","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.aninu.2024.06.007","article-title":"The dynamic distribution of the rectal microbiota in Holstein dairy calves provides a framework for understanding early-life gut health","volume":"19","author":"Huang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2004070","DOI":"10.1080\/19490976.2021.2004070","article-title":"The postnatal window is critical for the development of sex-specific metabolic and gut microbiota outcomes in offspring","volume":"13","author":"Daoust","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s40104-019-0376-z","article-title":"Enterococcus faecium NCIMB 10415 administration improves the intestinal health and immunity in neonatal piglets infected by enterotoxigenic Escherichia coli K88","volume":"10","author":"Peng","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1080\/19490976.2015.1067743","article-title":"Persistent effects of early infant diet and associated microbiota on the juvenile immune system","volume":"6","author":"Narayan","year":"2015","journal-title":"Gut Microbes"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1111\/jvim.14695","article-title":"Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves","volume":"31","author":"Gomez","year":"2017","journal-title":"J. Vet. Intern. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Bello-Gil, D., Audebert, C., Olivera-Ardid, S., P\u00e9rez-Cruz, M., Even, G., Khasbiullina, N., Gantois, N., Shilova, N., Merlin, S., and Costa, C. (2019). The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00342"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tian, S., Wang, J., Yu, H., Wang, J., and Zhu, W. (2019). Changes in Ileal Microbial Composition and Microbial Metabolism by an Early-Life Galacto-Oligosaccharides Intervention in a Neonatal Porcine Model. Nutrients, 11.","DOI":"10.3390\/nu11081753"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ayechu-Muruzabal, V., van de Kaa, M., Mukherjee, R., Garssen, J., Stahl, B., Pieters, R.J., Van\u2019t Land, B., Kraneveld, A.D., and Willemsen, L.E.M. (2022). Modulation of the Epithelial-Immune Cell Crosstalk and Related Galectin Secretion by DP3-5 Galacto-Oligosaccharides and \u03b2-3\u2032Galactosyllactose. Biomolecules, 12.","DOI":"10.3390\/biom12030384"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/msystems.00827-19","article-title":"Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance","volume":"5","author":"Richards","year":"2020","journal-title":"Msystems"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s40168-020-00980-0","article-title":"The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut","volume":"9","author":"Arnold","year":"2021","journal-title":"Microbiome"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3920\/BM2015.0114","article-title":"High purity galacto-oligosaccharides enhance specific Bifidobacterium species and their metabolic activity in the mouse gut microbiome","volume":"7","author":"Arthur","year":"2016","journal-title":"Benef. Microbes"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bier, A., Braun, T., Khasbab, R., Di Segni, A., Grossman, E., Haberman, Y., and Leibowitz, A. (2018). A High Salt Diet Modulates the Gut Microbiota and Short Chain Fatty Acids Production in a Salt-Sensitive Hypertension Rat Model. Nutrients, 10.","DOI":"10.3390\/nu10091154"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.aninu.2022.04.009","article-title":"Supplementation with galacto-oligosaccharides in early life persistently facilitates the microbial colonization of the rumen and promotes growth of preweaning Holstein dairy calves","volume":"10","author":"Chang","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yu, X., Ma, F., Dai, H., Liu, J., Hashem, N.M., and Sun, P. (2023). Effects of Different Galacto-Oligosaccharide Supplementation on Growth Performance, Immune Function, Serum Nutrients, and Appetite-Related Hormones in Holstein Calves. Animals, 13.","DOI":"10.3390\/ani13213366"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"114635","DOI":"10.1016\/j.anifeedsci.2020.114635","article-title":"Chromium yeast alleviates heat stress by improving antioxidant and immune function in Holstein mid-lactation dairy cows","volume":"269","author":"Shan","year":"2020","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1093\/bioinformatics\/btt593","article-title":"PEAR: A fast and accurate Illumina Paired-End reAd mergeR","volume":"30","author":"Zhang","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1089\/cmb.2017.0096","article-title":"cutPrimers: A New Tool for Accurate Cutting of Primers from Reads of Targeted Next Generation Sequencing","volume":"24","author":"Kechin","year":"2017","journal-title":"J. Comput. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: Improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2--approximately maximum-likelihood trees for large alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1093\/bioinformatics\/btu494","article-title":"STAMP: Statistical analysis of taxonomic and functional profiles","volume":"30","author":"Parks","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s40168-021-01221-8","article-title":"A multi-omics approach unravels metagenomic and metabolic alterations of a probiotic and synbiotic additive in rainbow trout (Oncorhynchus mykiss)","volume":"10","author":"Rasmussen","year":"2022","journal-title":"Microbiome"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8835","DOI":"10.1007\/s00216-015-9042-2","article-title":"Evaluation of changes induced in rice metabolome by Cd and Cu exposure using LC-MS with XCMS and MCR-ALS data analysis strategies","volume":"407","author":"Jaumot","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"D521","DOI":"10.1093\/nar\/gkl923","article-title":"HMDB: The Human Metabolome Database","volume":"35","author":"Wishart","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1093\/nar\/27.1.29","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"27","author":"Ogata","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1002\/jms.1777","article-title":"MassBank: A public repository for sharing mass spectral data for life sciences","volume":"45","author":"Horai","year":"2010","journal-title":"J. Mass Spectrom. JMS"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"32548","DOI":"10.1039\/D0RA05326K","article-title":"Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling","volume":"10","author":"Abdelrazig","year":"2020","journal-title":"RSC Adv."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"D527","DOI":"10.1093\/nar\/gkl838","article-title":"LMSD: LIPID MAPS structure database","volume":"35","author":"Sud","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1021\/acs.jproteome.5b00354","article-title":"Analysis of the Human Adult Urinary Metabolome Variations with Age, Body Mass Index, and Gender by Implementing a Comprehensive Workflow for Univariate and OPLS Statistical Analyses","volume":"14","author":"Roux","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/nprot.2011.319","article-title":"Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst","volume":"6","author":"Xia","year":"2011","journal-title":"Nat. Protoc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.3945\/jn.114.209486","article-title":"Galacto-oligosaccharides Protect the Intestinal Barrier by Maintaining the Tight Junction Network and Modulating the Inflammatory Responses after a Challenge with the Mycotoxin Deoxynivalenol in Human Caco-2 Cell Monolayers and B6C3F1 Mice","volume":"145","author":"Akbari","year":"2015","journal-title":"J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.aninu.2024.08.003","article-title":"Rumen-mammary gland axis and bacterial extracellular vesicles: Exploring a new perspective on heat stress in dairy cows","volume":"19","author":"Huang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s40104-022-00711-5","article-title":"Early-life galacto-oligosaccharides supplementation alleviates the small intestinal oxidative stress and dysfunction of lipopolysaccharide-challenged suckling piglets","volume":"13","author":"Tian","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1875796","DOI":"10.1080\/19490976.2021.1875796","article-title":"Blautia-a new functional genus with potential probiotic properties?","volume":"13","author":"Liu","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Eeckhaut, V., Wang, J., Van Parys, A., Haesebrouck, F., Joossens, M., Falony, G., Raes, J., Ducatelle, R., and Van Immerseel, F. (2016). The Probiotic Butyricicoccus pullicaecorum Reduces Feed Conversion and Protects from Potentially Harmful Intestinal Microorganisms and Necrotic Enteritis in Broilers. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01416"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/art.38892","article-title":"Decreased bacterial diversity characterizes the altered gut microbiota in patients with psoriatic arthritis, resembling dysbiosis in inflammatory bowel disease","volume":"67","author":"Scher","year":"2015","journal-title":"Arthritis Rheumatol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1136\/gutjnl-2020-322670","article-title":"Long-term dietary patterns are associated with pro-inflammatory and anti-inflammatory features of the gut microbiome","volume":"70","author":"Bolte","year":"2021","journal-title":"Gut"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.7150\/ijbs.35980","article-title":"Dysbiosis of the Gut Microbiome is associated with Tumor Biomarkers in Lung Cancer","volume":"15","author":"Liu","year":"2019","journal-title":"Int. J. Biol. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1136\/gutjnl-2020-323071","article-title":"Gut microbiota-derived metabolites as central regulators in metabolic disorders","volume":"70","author":"Agus","year":"2021","journal-title":"Gut"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Magn\u00fasd\u00f3ttir, S., Ravcheev, D., de Cr\u00e9cy-Lagard, V., and Thiele, I. (2015). Systematic genome assessment of B-vitamin biosynthesis suggests co-operation among gut microbes. Front. Genet., 6.","DOI":"10.3389\/fgene.2015.00148"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1136\/gutjnl-2021-325753","article-title":"Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: Effect of biotin and prebiotic supplementation on improved metabolism","volume":"71","author":"Belda","year":"2022","journal-title":"Gut"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Gu, X., Al Dubayee, M., Alshahrani, A., Masood, A., Benabdelkamel, H., Zahra, M., Li, L., Abdel Rahman, A.M., and Aljada, A. (2020). Distinctive Metabolomics Patterns Associated with Insulin Resistance and Type 2 Diabetes Mellitus. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.609806"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Wang, H., Dong, P., Liu, X., Zhang, Z., Li, H., Li, Y., Zhang, J., Dai, L., and Wang, S. (2022). Active Peptide AR-9 from Eupolyphaga sinensis Reduces Blood Lipid and Hepatic Lipid Accumulation by Restoring Gut Flora and Its Metabolites in a High Fat Diet-Induced Hyperlipidemia Rat. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.918505"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cheng, N., Chen, S., Liu, X., Zhao, H., and Cao, W. (2019). Impact of SchisandraChinensis Bee Pollen on Nonalcoholic Fatty Liver Disease and Gut Microbiota in HighFat Diet Induced Obese Mice. Nutrients, 11.","DOI":"10.3390\/nu11020346"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wu, X., Xu, W., Zhou, T., Cao, N., Ni, J., Zou, T., Liang, Z., Wang, X., and Fenech, M. (2016). The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene-Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071003"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/jdv.15448","article-title":"Fumaric acid ester-induced T-cell lymphopenia in the real-life treatment of psoriasis","volume":"33","author":"Dickel","year":"2019","journal-title":"J. Eur. Acad. Dermatol. Venereol. JEADV"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Hyldgaard, M., Mygind, T., Piotrowska, R., Foss, M., and Meyer, R.L. (2015). Isoeugenol has a non-disruptive detergent-like mechanism of action. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00754"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Rangel-Huerta, O.D., Ivanova, L., Uhlig, S., Sivertsvik, M., Sone, I., Fern\u00e1ndez, E.N., and F\u00e6ste, C.K. (2021). Impact of Plasma-Activated Water Treatment on Quality and Shelf-Life of Fresh Spinach Leaves Evaluated by Comprehensive Metabolomic Analysis. Foods, 10.","DOI":"10.3390\/foods10123067"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Wang, W., Geng, M., Zhu, C., Huang, L., Zhang, Y., Zhang, T., Zhao, C., Zhang, T., Du, X., and Wang, N. (2022). Protective Effects and Mechanism of a Novel Probiotic Strain Ligilactobacillus salivarius YL20 against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and In Vivo. Nutrients, 14.","DOI":"10.3390\/nu14183827"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nri.2016.42","article-title":"Gut microbiota, metabolites and host immunity","volume":"16","author":"Rooks","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.chom.2016.07.001","article-title":"Gut Microbial Metabolites Fuel Host Antibody Responses","volume":"20","author":"Kim","year":"2016","journal-title":"Cell Host Microbe"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/1\/126\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:12:47Z","timestamp":1767676367000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/1\/126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,1]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ani16010126"],"URL":"https:\/\/doi.org\/10.3390\/ani16010126","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,1]]}}}