{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:01:30Z","timestamp":1791414090299,"version":"4.3.3"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015600","name":"Guangdong Ocean University","doi-asserted-by":"publisher","award":["030301022301"],"award-info":[{"award-number":["030301022301"]}],"id":[{"id":"10.13039\/501100015600","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Southern Marine Science and Engineering Guangdong Laboratory","award":["ZJW2023-10"],"award-info":[{"award-number":["ZJW2023-10"]}]},{"name":"Southern Marine Science and Engineering Guangdong Laboratory","award":["ZJW-2022-05"],"award-info":[{"award-number":["ZJW-2022-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The greater amberjack (Seriola dumerili) is an economically important marine species that is prone to bacterial infections, resulting in high mortality rates and substantial economic losses. In this study, a virulent bacterial strain, Vh-2, was isolated from diseased greater amberjack and identified as Vibrio harveyi. Experimental infections caused high mortality and severe splenic damage characterized by tissue necrosis, abnormal pigment deposition, cellular disintegration, and extensive immune cell infiltration. A virulence gene analysis revealed that Vh-2 harbored multiple virulence-associated genes such as toxR, toxS, vhpA, vhpB, vhhA, vhhB, luxR, and pap6. Antibiotic susceptibility testing demonstrated ampicillin resistance but sensitivity to ceftriaxone, florfenicol, and meropenem. Transcriptomic profiling of infected spleens identified 396 differentially expressed genes (DEGs) compared to the control group, of which 293 were upregulated and 103 were downregulated. A functional enrichment analysis indicated that these genes were primarily involved in cell cycle regulation, DNA repair, metabolic processes, and immune-related pathways. These findings enhance our understanding of V. harveyi pathogenesis and immune responses of S. dumerili and provide new insights into the prevention and control of V. harveyi infections in marine fish.<\/jats:p>","DOI":"10.3390\/ani16020184","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T12:56:55Z","timestamp":1767877015000},"page":"184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pathogenic Characterization and Host Immune Response to Vibrio harveyi in Diseased Seriola dumerili"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6296-7069","authenticated-orcid":false,"given":"Rizhao","family":"Zhang","sequence":"first","affiliation":[{"name":"Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingbo","family":"Hu","sequence":"additional","affiliation":[{"name":"Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunpeng","family":"Lan","sequence":"additional","affiliation":[{"name":"Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haimin","family":"Tan","sequence":"additional","affiliation":[{"name":"Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Huang","sequence":"additional","affiliation":[{"name":"Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China"},{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China"},{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"737353","DOI":"10.1016\/j.aquaculture.2021.737353","article-title":"A live attenuated strain of HY9901\u0394vscB provides protection against Vibrio alginolyticus in pearl gentian grouper (\u2640Epinephelus fuscoguttatus \u00d7 \u2642Epinephelus lanceolatu)","volume":"546","author":"Pang","year":"2022","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3147\/jsfp.51.1","article-title":"Vibrio harveyi Infection in Farmed Greater Amberjack Seriola dumerili","volume":"51","author":"Minami","year":"2016","journal-title":"Fish Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s42995-020-00037-z","article-title":"Vibrio harveyi: A serious pathogen of fish and invertebrates in mariculture","volume":"2","author":"Zhang","year":"2020","journal-title":"Mar. 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