{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T03:13:46Z","timestamp":1793416426769,"version":"4.5.0"},"reference-count":72,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2023YFD1801100"],"award-info":[{"award-number":["2023YFD1801100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shaanxi Province Key R&D Program Projects","award":["2024NC2-GJHX-13"],"award-info":[{"award-number":["2024NC2-GJHX-13"]}]},{"name":"Shaanxi Livestock and Poultry Breeding Double-chain Fusion Key Project","award":["2022GD-TSLD-46-0103"],"award-info":[{"award-number":["2022GD-TSLD-46-0103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study investigated the metabolic mechanisms driving physiological functional remodeling in RFM by analyzing plasma biochemical parameters and metabolomic profiles at key peripartum timepoints (21 and 7 d prepartum and 4 h postpartum), integrated with placental and fetal membrane metabolic characteristics. The results revealed that RFM cows exhibited significant negative energy balance (NEB) as early as 21 days before parturition, characterized by elevated plasma levels of non-esterified fatty acids, \u03b2-hydroxybutyrate, and malondialdehyde, alongside reduced activity of antioxidant enzymes (GSH-Px, CAT) (p \u2264 0.05). Metabolomic analysis demonstrated persistent lipid metabolism dysregulation, amino acid imbalance, and nucleotide metabolism disturbances in RFM cows from 21 days prepartum to 4 h postpartum, indicating premature mobilization of adipose and muscle tissues. Further metabolomic analyses of the placenta and fetal membranes confirmed that metabolic dysfunction compromises energy supply during parturition, adversely affecting immune homeostasis and extracellular matrix degradation in the placenta and fetal membranes of RFM dairy cows. These physiological dysfunctions have the potential to impede the timely expulsion of fetal membranes after calving. In conclusion, RFM is closely associated with early-onset metabolic dysfunction during the periparturient period, where insufficient energy supply due to NEB, oxidative stress, and immune-endocrine disruptions collectively impair normal fetal membrane detachment.<\/jats:p>","DOI":"10.3390\/ani16020229","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T09:27:12Z","timestamp":1768296432000},"page":"229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Early-Onset Negative Energy Balance in Transition Dairy Cows Increases the Incidence of Retained Fetal Membranes"],"prefix":"10.3390","volume":"16","author":[{"given":"Zhihong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhao","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfeng","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/mrd.22635","article-title":"Cellular and molecular mechanisms involved in placental separation in the bovine: A review","volume":"83","author":"Attupuram","year":"2016","journal-title":"Mol. Reprod. Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.theriogenology.2021.08.036","article-title":"Assessing the consequences and economic impact of retained placenta in Holstein dairy cattle","volume":"175","author":"Mahnani","year":"2021","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1136\/vr.100317","article-title":"Effects of periparturient events on subsequent culling and fertility in eight UK dairy herds","volume":"170","author":"Hayes","year":"2012","journal-title":"Vet. Rec."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.theriogenology.2009.06.001","article-title":"Relationship between serum nonesterified fatty acids at calving and the incidence of periparturient diseases in Holstein dairy cows","volume":"72","author":"Melendez","year":"2009","journal-title":"Theriogenology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/rda.14115","article-title":"Latest update on predictive indicators, risk factors and \u2018Omic\u2019 technologies research of retained placenta in dairy cattle\u2014A review","volume":"57","author":"Amin","year":"2022","journal-title":"Reprod. Domest. Anim."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.theriogenology.2021.01.016","article-title":"Observed and expected combined effects of metritis and other postpartum diseases on time to conception and rate of conception failure in first lactation cows in Iran","volume":"164","author":"Ehsanollah","year":"2021","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.3168\/jds.2016-11565","article-title":"Estimating US dairy clinical disease costs with a stochastic simulation model","volume":"100","author":"Liang","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.2460\/javma.252.12.1485","article-title":"Cost of retained fetal membranes for dairy herds in the United States","volume":"252","author":"Gohary","year":"2018","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.theriogenology.2016.04.043","article-title":"The bovine placenta in vivo and in vitro","volume":"86","author":"Haeger","year":"2016","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1159\/000072720","article-title":"Collagen types I, III and IV in the placentome and interplacentomal maternal and fetal tissues in normal cows and in cattle with retention of fetal membranes","volume":"174","author":"Boos","year":"2003","journal-title":"Cells Tissues Organs"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.theriogenology.2013.06.004","article-title":"Bovine placenta: A review on morphology, components, and defects from terminology and clinical perspectives","volume":"80","author":"Peter","year":"2013","journal-title":"Theriogenology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1071\/RD23119","article-title":"Understanding placentation in ruminants: A review focusing on cows and sheep","volume":"36","author":"Johnson","year":"2023","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1530\/REP-11-0204","article-title":"Gene expression profiling of bovine peripartal placentomes: Detection of molecular pathways potentially involved in the release of foetal membranes","volume":"143","author":"Streyl","year":"2012","journal-title":"Reproduction"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1136\/vr.165.21.615","article-title":"Structure and function of the bovine fetomaternal unit in relation to the causes of retained fetal membranes","volume":"165","author":"McNaughton","year":"2009","journal-title":"Vet. Rec."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.anireprosci.2006.08.007","article-title":"The effect of nutritional management of the dairy cow on reproductive efficiency","volume":"96","author":"Roche","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rafa, H., Oroian, I., Cozma, O.M., Morohoschi, A.G., Dumitras, D.A., \u0218tef\u0103nu\u021b, C.L., Neagu, D., Borzan, A., and Andrei, S. (2024). Peripartal changes of metabolic and hormonal parameters in Romanian spotted cows and their relation with retained fetal membranes. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1409666"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"100017","DOI":"10.1016\/j.animal.2020.100017","article-title":"Subclinical hypocalcaemia follows specific time-related and severity patterns in post-partum Holstein cows","volume":"15","author":"Tsiamadis","year":"2021","journal-title":"Animal"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5878","DOI":"10.3168\/jds.2010-3391","article-title":"Associations of prepartum plasma cortisol, haptoglobin, fecal cortisol metabolites, and nonesterified fatty acids with postpartum health status in Holstein dairy cows","volume":"94","author":"Huzzey","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106294","DOI":"10.1016\/j.prevetmed.2024.106294","article-title":"Association between non-esterified fatty acids and calcium concentrations at calving with early lactation clinical diseases, fertility and culling in grazing dairy cows in Uruguay","volume":"230","author":"Cruz","year":"2024","journal-title":"Prev. Vet. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s40104-017-0174-4","article-title":"Adipose tissue lipolysis and remodeling during the transition period of dairy cows","volume":"8","author":"Contreras","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6858","DOI":"10.3168\/jds.2021-21526","article-title":"Associations between time in the close-up group and milk yield, milk components, reproductive performance, and culling of Holstein dairy cows fed acidogenic diets: A multisite study","volume":"105","author":"Venjakob","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tufarelli, V., Puva\u010da, N., Glamo\u010di\u0107, D., Pugliese, G., and Colonna, M.A. (2024). The Most Important Metabolic Diseases in Dairy Cattle during the Transition Period. Animals, 14.","DOI":"10.3390\/ani14050816"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6737","DOI":"10.3168\/jds.2016-10969","article-title":"Insulin signaling, inflammation, and lipolysis in subcutaneous adipose tissue of transition dairy cows either overfed energy during the prepartum period or fed a controlled-energy diet","volume":"99","author":"Mann","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Martens, H. (2023). Invited Review: Increasing Milk Yield and Negative Energy Balance: A Gordian Knot for Dairy Cows?. Animals, 13.","DOI":"10.3390\/ani13193097"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.theriogenology.2020.01.042","article-title":"Modulation of immune function in the bovine uterus peripartum","volume":"150","author":"Pascottini","year":"2020","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.cell.2015.02.010","article-title":"Homeostasis, inflammation, and disease susceptibility","volume":"160","author":"Kotas","year":"2015","journal-title":"Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"23250","DOI":"10.1038\/s41598-024-74947-8","article-title":"Mechanism of collagen type IV regulation by focal adhesion kinase during retained fetal membranes in dairy cows","volume":"14","author":"Luo","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.theriogenology.2010.11.019","article-title":"Pfarrer C: Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention","volume":"75","author":"Dilly","year":"2011","journal-title":"Theriogenology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100796","DOI":"10.1016\/j.animal.2023.100796","article-title":"Review: Implantation and placentation in ruminants","volume":"17","author":"Davenport","year":"2023","journal-title":"Animal"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.3168\/jds.S0022-0302(06)72197-X","article-title":"Major advances in our understanding of nutritional influences on bovine health","volume":"89","author":"Goff","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Turk, R., Rosic, N., Beer, L.B., and Vince, S. (2024). Effects of Summer Heat on Adipose Tissue Activity in Periparturient Simmental Cows. Metabolites, 14.","DOI":"10.3390\/metabo14040207"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1093\/biolre\/ioaa069","article-title":"Experimentally Induced Endometritis Impairs the Developmental Capacity of Bovine Oocytes\u2020","volume":"103","author":"Dickson","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9054","DOI":"10.3168\/jds.2021-21327","article-title":"Characterization of metabolic profile, health, milk production, and reproductive outcomes of dairy cows diagnosed with concurrent hyperketonemia and hypoglycemia","volume":"105","author":"Hubner","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.3168\/jds.2014-8237","article-title":"Diseases, reproductive performance, and changes in milk production associated with subclinical ketosis in dairy cows: A meta-analysis and review","volume":"97","author":"Raboisson","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1262\/jrd.1056S29","article-title":"Monitoring metabolic health of dairy cattle in the transition period","volume":"56","author":"LeBlanc","year":"2010","journal-title":"J. Reprod. Dev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7158","DOI":"10.3168\/jds.2012-5812","article-title":"Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease","volume":"95","author":"Martinez","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3326","DOI":"10.1093\/jas\/skz209","article-title":"Effects of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil activity in dairy cows with subclinical hypocalcemia1","volume":"97","author":"Zhang","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1111\/j.1439-0531.2008.01096.x","article-title":"Cervical ripening and parturition in cows are driven by a cascade of pro-inflammatory cytokines","volume":"44","author":"Everts","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1054\/plef.2000.0235","article-title":"The levels of lipid peroxidation products in bovine retained and not retained placenta","volume":"64","author":"Kankofer","year":"2001","journal-title":"Prostag. Leukotr. ESS"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.tvjl.2009.09.003","article-title":"Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture","volume":"186","author":"Pedernera","year":"2010","journal-title":"Vet. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.cvfa.2013.03.002","article-title":"Significance of metabolic stress, lipid mobilization, and inflammation on transition cow disorders","volume":"29","author":"Sordillo","year":"2013","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"D\u017eermeikait\u0117, K., Kri\u0161tolaityt\u0117, J., Sutkevi\u010dien\u0117, N., Vilkonien\u0117, T., Vai\u010diulien\u0117, G., Reke\u0161i\u016bt\u0117, A., Girdauskait\u0117, A., Arlauskait\u0117, S., Bajcsy, \u00c1.C., and Antanaitis, R. (2025). Relationships Among In-Line Milk Fat-to-Protein Ratio, Metabolic Profile, and Inflammatory Biomarkers During Early Stage of Lactation in Dairy Cows. Vet. Sci., 12.","DOI":"10.3390\/vetsci12020187"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e13935","DOI":"10.1111\/asj.13935","article-title":"The role of endoplasmic reticulum stress in metabolic diseases and mammary epithelial cell homeostasis in dairy cows","volume":"95","author":"Yonekura","year":"2024","journal-title":"Anim. Sci. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s44154-023-00088-z","article-title":"Effects of perinatal stress on the metabolites and lipids in plasma of dairy goats","volume":"3","author":"Huang","year":"2023","journal-title":"Stress Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.aninu.2024.04.013","article-title":"Rumen microbiota succession throughout the perinatal period and its association with postpartum production traits in dairy cows: A review","volume":"18","author":"Zhao","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Fluck, A.C., Macagnan, R., Skonieski, F.R., Costa, O.A.D., Cardinal, K.M., Borba, L.P.d., Schmitz, B., and Fischer, V. (2025). Is there an appropriate energy level in the diet during the cow transition period? A systematic review and meta-analysis. J. Dairy Res., 1\u201315.","DOI":"10.1017\/S002202992510112X"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.3168\/jds.S0022-0302(93)77729-2","article-title":"Etiology of Lipid-Related Metabolic Disorders in Periparturient Dairy Cows","volume":"76","author":"Grummer","year":"1993","journal-title":"J. Dairy S."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"ADSA Foundation Scholar Award. Biology of dairy cows during the transition period: The final frontier?","volume":"82","author":"Drackley","year":"1999","journal-title":"J. Dairy Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3390\/dairy5010019","article-title":"Assessing Serum Vaspin Dynamics in Dairy Cows during Late Pregnancy and Early Lactation in Relation to Negative Energy Balance","volume":"5","author":"Naji","year":"2024","journal-title":"Dairy"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"s165","DOI":"10.1017\/S1751731119003185","article-title":"Review: Lipid biology in the periparturient dairy cow: Contemporary perspectives","volume":"14","author":"McFadden","year":"2020","journal-title":"Animal"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"8887","DOI":"10.3168\/jds.2025-26442","article-title":"Metabolic lipid alterations in subclinical ketotic dairy cows: A multisample lipidomic approach","volume":"108","author":"He","year":"2025","journal-title":"J. Dairy Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"100988","DOI":"10.1016\/j.jbior.2023.100988","article-title":"The role and regulation of phospholipase D in metabolic disorders","volume":"91","author":"Park","year":"2024","journal-title":"Adv. Biol. Reg."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"101018","DOI":"10.1016\/j.plipres.2019.101018","article-title":"Mammalian phospholipase D: Function, and therapeutics","volume":"78","author":"McDermott","year":"2020","journal-title":"Prog. Lip. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2527\/asasann.2017.043","article-title":"043 Metabolomics uncovers serum biomarkers that can predict the risk of retained placenta in transition dairy cows","volume":"95","author":"Dervishi","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_55","unstructured":"Berg, J.M., and Tymoczko, J.L. (2019). Stryer L: Phospholipid Metabolism. Biochemistry, W. H. Freeman and Company. [9th ed.]."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"822","DOI":"10.3168\/jds.2022-22068","article-title":"Invited review: Muscle protein breakdown and its assessment in periparturient dairy cows","volume":"106","author":"Sadri","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.3168\/jds.2024-25324","article-title":"Longitudinal characterization of the muscle metabolome in dairy cows during the transition from lactation cessation to lactation resumption","volume":"108","author":"Sadri","year":"2025","journal-title":"J. Dairy Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"11655","DOI":"10.3168\/jds.2024-24915","article-title":"Production responses of multiparous dairy cattle with differing prepartum muscle reserves and supplementation of branched-chain volatile fatty acids","volume":"107","author":"Gouveia","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/01652176.2022.2145619","article-title":"Potential prognostic markers of retained placenta in dairy cows identified by plasma metabolomics coupled with clinical laboratory indicators","volume":"42","author":"Li","year":"2022","journal-title":"Vet. Quart."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1093\/nar\/gkv047","article-title":"Regulation of mammalian nucleotide metabolism and biosynthesis","volume":"43","author":"Lane","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_61","first-page":"499","article-title":"The Interplay between Oxidative Stress and Fatty Acids Profile in Romanian Spotted Cows with Placental Retention","volume":"11","author":"Andrei","year":"2024","journal-title":"Vet. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"106549","DOI":"10.1016\/j.anireprosci.2020.106549","article-title":"Effects of non-esterified fatty acids on relative abundance of prostaglandin E2 and F2\u03b1 synthesis-related mRNA transcripts and protein in endometrial cells of cattle in vitro","volume":"221","author":"Qin","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Chankeaw, W., Lignier, S., Richard, C., Ntallaris, T., Raliou, M., Guo, Y., Plassard, D., Bevilacqua, C., Sandra, O., and Andersson, G. (2021). Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): Impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells. BMC Genom., 22.","DOI":"10.1186\/s12864-021-07713-z"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.1021\/acs.jproteome.9b00706","article-title":"Metabolomics of Milk Reflects a Negative Energy Balance in Cows","volume":"19","author":"Xu","year":"2020","journal-title":"J. Proteome Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.3168\/jds.2010-3707","article-title":"Performance and metabolic profile of dairy cows during a lactational and deliberately induced negative energy balance with subsequent realimentation","volume":"94","author":"Gross","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4795","DOI":"10.3168\/jds.2019-17777","article-title":"Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation","volume":"103","author":"Xu","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1152\/physiolgenomics.90394.2008","article-title":"Pleiotropic effects of negative energy balance in the postpartum dairy cow on splenic gene expression: Repercussions for innate and adaptive immunity","volume":"39","author":"Morris","year":"2009","journal-title":"Physiol. Genom."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.theriogenology.2018.11.029","article-title":"Expression of proinflammatory cytokines IL-1\u03b2, IL-6 and TNF\u03b1 in the retained placenta of mares","volume":"126","author":"Jaworska","year":"2019","journal-title":"Theriogenology"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s12935-023-03143-x","article-title":"The interconnected roles of TRIM21\/Ro52 in systemic lupus erythematosus, primary Sj\u00f6gren\u2019s syndrome, cancers, and cancer metabolism","volume":"23","author":"Hsu","year":"2023","journal-title":"Cancer Cell Inter."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40104-016-0085-9","article-title":"Occurrence of retained placenta is preceded by an inflammatory state and alterations of energy metabolism in transition dairy cows","volume":"7","author":"Dervishi","year":"2016","journal-title":"J. Anim. Sci. Biotechno."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/rda.13747","article-title":"Activity of selected glycosidases and availability of their substrates in bovine placenta during pregnancy and parturition with and without retained foetal membranes","volume":"55","author":"Wawrzykowski","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/j.1939-1676.2010.0473.x","article-title":"Physiology and Treatment of Retained Fetal Membranes in Cattle","volume":"24","author":"Beagley","year":"2010","journal-title":"J. Vet. Intern. 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