{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T17:03:00Z","timestamp":1769187780330,"version":"3.49.0"},"reference-count":60,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cHuxiang Young Talents Plan\u201d Project of Hunan Province","award":["2022RC1157"],"award-info":[{"award-number":["2022RC1157"]}]},{"name":"Hunan Major Basic Research Project","award":["2024JC0007"],"award-info":[{"award-number":["2024JC0007"]}]},{"name":"College of Animal Science and Technology, Hunan Agricultural University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Weaning stress frequently compromises intestinal integrity and nutrient absorption in piglets and induces structural perturbations in the gut microbiota. This study investigated the effects of dietary Paramylon, a linear \u03b2-1,3-glucan from Euglena gracilis, on growth and intestinal function. A total of 32 healthy, 21-day-old weaned piglets (male, castrated, Duroc \u00d7 Landrace \u00d7 Yorkshire) were randomly allocated to four groups: control, 0.025%, 0.05%, and 0.1% Paramylon (8 pigs\/group). The results showed that 0.05% dietary Paramylon quadratically increased average daily gain and feed intake (p &lt; 0.05). Serum TC content significantly increased, while the serum urea level significantly decreased (p &lt; 0.05). This optimal dose was used for mechanistic exploration. Dietary 0.05% Paramylon notably enhanced ileal morphology, increasing the villus height to crypt depth ratio (p &lt; 0.01) and significantly improving apparent nutrient digestibility and ileal \u03b2-amylase activity (p &lt; 0.05). The expression levels of SLC7A1 and GLUT2 in ileum tissues were significantly upregulated (p &lt; 0.05). The expression level of SLC7A7 in the liver was also increased (p &lt; 0.05). This nutrient transport-promoting effect was further confirmed in IPEC-J2 cells, which manifested because 10 ng\/mL of pure Paramylon significantly upregulated the gene expressions of SLC38A2, EAAT3, PEPT1, and GLUT2 (p &lt; 0.05). KEGG enrichment analysis on the ileum indicated that differentially abundant metabolites were enriched in amino acid-related metabolic pathways. Furthermore, the 16s sequencing results revealed that Romboutsia was significantly enriched in the Paramylon group. In conclusion, Paramylon, as an effective dietary supplement, helps promote nutrient digestion and absorption in weaned piglets and contributes to maintaining intestinal health.<\/jats:p>","DOI":"10.3390\/ani16020304","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:58:54Z","timestamp":1768834734000},"page":"304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Dietary Paramylon on Nutrient Digestion and Absorption and Intestinal Health of Weaned Piglets"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7653-8963","authenticated-orcid":false,"given":"Tianjiao","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China"},{"name":"Key Laboratory of Livestock and Poultry Resources Evaluation and Utilization, Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6919-6334","authenticated-orcid":false,"given":"Zheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Kemin (China) Technologies Co., Ltd., 25 Qinshi Road, Sanzao, Zhuhai 519040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangbao","family":"Shu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China"},{"name":"Key Laboratory of Livestock and Poultry Resources Evaluation and Utilization, Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1852-1042","authenticated-orcid":false,"given":"Jie","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China"},{"name":"Key Laboratory of Livestock and Poultry Resources Evaluation and Utilization, Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Bin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s40104-015-0042-z","article-title":"A review of the effects of dietary organic acids fed to swine","volume":"6","author":"Suiryanrayna","year":"2015","journal-title":"J. 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