{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T12:56:06Z","timestamp":1769259366915,"version":"3.49.0"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32302997"],"award-info":[{"award-number":["32302997"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["ZR2023QC113"],"award-info":[{"award-number":["ZR2023QC113"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Yantai Science and Technology Innovation Development Plan","award":["2022XDRH022"],"award-info":[{"award-number":["2022XDRH022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Thamnaconus septentrionalis is an emerging commercially important aquaculture species in China, distributed extensively in the Indo-West Pacific Ocean. Recently, because of the seriously declining population and considerable economic potential of this aquaculture fish, increasing attention has been paid to the conservation and development of T. septentrionalis. Artificial fish breeding is essential and has become progressively implemented on local farms in China, which benefits the protection of T. septentrionalis resources and facilitates the development of its fishery industry. Previous studies have demonstrated that temperature could significantly influence ovary development in T. septentrionalis. However, the potential molecular mechanisms underlying the influence of temperature on testis development in T. septentrionalis have been scarcely studied. Thus, this study comprehensively explores the effects of temperature on testis development in T. septentrionalis using histological observation and transcriptomic techniques. Histological and transmission electron microscopy analyses indicated that T. septentrionalis testes, undergoing a rise in temperature, developed from phase III to IV. Transcriptomic analysis identified 315 differentially expressed genes, including 200 upregulated and 115 downregulated genes. Moreover, rising temperatures may enhance testis development by regulating steroid hormone biosynthesis, cellular senescence, and nucleotide metabolism. The upregulation of four genes (hsd11b2, cyp11b, cyp11a, and hsd17b3) involved in the steroid hormone biosynthesis process may significantly contribute to the increased level of testosterone and 11-keto-testosterone. This study is the first to elucidate the potential molecular mechanism involved in T. septentrionalis testis development induced by temperature, offering valuable and novel insights for its artificial breeding and fishery resources conservation.<\/jats:p>","DOI":"10.3390\/ani16020327","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T11:11:17Z","timestamp":1768993877000},"page":"327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics Analysis of Testis Development in Thamnaconus septentrionalis Responding to a Rise in Temperature"],"prefix":"10.3390","volume":"16","author":[{"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Ocean, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueli","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Ocean, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4820-9108","authenticated-orcid":false,"given":"Wengang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Ocean, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiulong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Ocean, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Bian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9324-2931","authenticated-orcid":false,"given":"Yanqing","family":"Wu","sequence":"additional","affiliation":[{"name":"East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5649-9336","authenticated-orcid":false,"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Ocean, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/1755-0998.13183","article-title":"Chromosome-level genome assembly of the greenfin horse-faced filefish (Thamnaconus septentrionalis) using Oxford Nanopore PromethION sequencing and Hi-C technology","volume":"20","author":"Bian","year":"2020","journal-title":"Mol. Ecol. Res."},{"key":"ref_2","first-page":"409","article-title":"Evaluation of Thamnaconus septentrionalis Resources and their Rational Utilization","volume":"4","author":"Zhan","year":"1986","journal-title":"Aquac. Fish"},{"key":"ref_3","unstructured":"Fisheries Bureau of Ministry of Agriculture (2024). China Fisheries Yearbook 2024, China Agriculture Press."},{"key":"ref_4","first-page":"11","article-title":"Histological observation on the gonadal development of the parental Thamnaconus modestus during early reproductive regulation","volume":"46","author":"Mao","year":"2024","journal-title":"J. Fish. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Flores, A., Wiff, R., Brown, D., and Larrea-Meza, S. (2000). Reproductive traits of female of pink cusk-eel (Genypterus blacodes Schneider, 1801) in the fjords of the Chilean Patagonia. Estuar. Coast. Shelf Sci., 233.","DOI":"10.1016\/j.ecss.2019.106554"},{"key":"ref_6","first-page":"65","article-title":"Regulation of ovarian development and function in teleosts","volume":"35","author":"Urbatzka","year":"2011","journal-title":"Horm. Reprod. Vertebr."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Fu, W., Li, Z., Ren, X., Liu, F., Liu, W., Liu, J., Peng, L., and Xiao, Y. (2025). Effects of high temperature on gonadal development of gynogenetic Megalobrama amblycephala and acquisition of fertile pseudo-male fish. Aquaculture, 606.","DOI":"10.1016\/j.aquaculture.2025.742588"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Guan, Q., Xu, H., Huang, L., Zulfahmi, I., Kisha, M.S., Masroor, W., Zhao, C., and Cao, Q. (2026). Regulatory mechanisms of temperature on the gonadal development of fish. Aquaculture, 613.","DOI":"10.1016\/j.aquaculture.2025.743382"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"He, K., Li, Z., Yan, H., Shi, L., Yang, H., Liu, Q., Song, K., Hu, Y., Wang, B., and Yang, S. (2025). Cold temperature delays ovarian development of largemouth bass by inhibiting sex hormone release, angiogenesis, apoptosis and autophagy during out-of-season reproduction. Comp. Biochem. Phys. Part A, 301.","DOI":"10.1016\/j.cbpa.2024.111795"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Peng, Y., Yan, L., Zhao, C., Zhang, B., Zhang, B., and Qiu, L. (2025). Effects of Temperature and Salinity on Ovarian Development and Differences in Energy Metabolism Between Reproduction and Growth During Ovarian Development in the Lateolabrax maculatus. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26178295"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Li, T., Chen, Q., Zhang, Q., Feng, T., Zhang, J., Lin, Y., Yang, P., He, S., and Zhang, H. (2022). Transcriptomic Analysis on the Effects of Altered Water Temperature Regime on the Fish Ovarian Development of Coreius guichenoti under the Impact of River Damming. Biology, 11.","DOI":"10.3390\/biology11121829"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Huang, J., Liao, S., Su, Y., Li, M., Hu, J., Han, L., Jiang, Y., Yang, M., Zhang, Y., and Li, S. (2024). Effects of temperature on ovarian development of mandarin fish (Siniperca chuatsi) and hormone therapy to induce its ovulation out of breeding season. Aquacult. Rep., 37.","DOI":"10.1016\/j.aqrep.2024.102271"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cai, S., Li, Y., Xu, R., He, C., Chen, K., Chen, J., Hu, W., and Jiang, M. (2023). Transcriptome analysis provides new insights into ovarian follicle growth in common carp (Cyprinus carpio). Aquacult. Rep., 33.","DOI":"10.1016\/j.aqrep.2023.101864"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Pino Martinez, E., Braanaas, M.F., Balseiro, P., Kraugerud, M., Pedrosa, C., Imsland, A.K.D., and Handeland, S.O. (2022). Constant High Temperature Promotes Early Changes in Testis Development Associated with Sexual Maturation in Male Atlantic Salmon (Salmo salar L.) Post-Smolts. Fishes, 7.","DOI":"10.3390\/fishes7060341"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1007\/s11802-023-5529-1","article-title":"Testis Development and Ultrastructural Features During Spermatogenesis in Cultured Small Yellow Croaker, Larimichthys Polyactis","volume":"22","author":"Zheng","year":"2023","journal-title":"J. Ocean. Univ. China"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1093\/icesjms\/fsu155","article-title":"Using the gonadosomatic index to estimate the maturity ogive: Application to Chilean hake (Merluccius gayi gayi)","volume":"72","author":"Flores","year":"2015","journal-title":"ICES J. Mar. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/jfb.15449","article-title":"A study on the seasonal cyclicity of ovarian development in adult himalayan snow trout, Schizothorax plagiostomus","volume":"103","author":"Sharma","year":"2023","journal-title":"J. Fish Biol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Liu, Y., Xu, W., Zhang, X., Wang, J., Chen, X., Yu, X., Zeng, J., Wu, Y., and Liu, L. (2025). The molecular mechanism of ovary development in Thamnaconus septentrionalis induced by rising temperature via transcriptomics and metabolomics analysis. Front. Mar. Sci., 12.","DOI":"10.3389\/fmars.2025.1556002"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.molimm.2015.06.028","article-title":"Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus","volume":"67","author":"Li","year":"2015","journal-title":"Mol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Meuer, S., Wittwer, C., and Nakagawara, K. (2001). Quantification on the LightCycler. Rapid Cycle Real-Time PCR, Methods and Applications, Springer Press.","DOI":"10.1007\/978-3-642-59524-0"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, X., Meng, B., Zhang, Z., Wei, L., Chang, W., Wang, Y., Zhang, K., Li, T., and Lu, K. (2024). qPrimerDB 2.0: An updated comprehensive gene-specific qPCR primer database for 1172 organisms. Nucleic Acids Res., 53.","DOI":"10.1093\/nar\/gkae684"},{"key":"ref_24","first-page":"35","article-title":"Study on Thamnaconus septentrionalis under industrial aquaculture condition","volume":"44","author":"Liu","year":"2017","journal-title":"Fish. Mod."},{"key":"ref_25","first-page":"123","article-title":"Current research status of Thamnaconus septentrionalis","volume":"40","author":"Chen","year":"2025","journal-title":"Fish. Inf. Strategy"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1111\/are.14407","article-title":"Effect of different water temperatures on sex ratio, gonad development and production parameters of common carp (Cyprinus carpio L.)","volume":"51","author":"Minya","year":"2020","journal-title":"Aquac. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Housh, M.J., Telish, J., Forsgren, K.L., and Lema, S.C. (2024). Fluctuating and Stable High Temperatures Differentially Affect Reproductive Endocrinology of Female Pupfish. Integr. Org. Biol., 6.","DOI":"10.1093\/iob\/obae003"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Chen, H., Wang, Y., Mei, Y., Huang, L., Wang, H., and Ji, X. (2020). Gonad development examination of high-temperature\u2013treated genetically female Nile tilapia. Aquaculture, 515.","DOI":"10.1016\/j.aquaculture.2019.734535"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s00227-017-3116-y","article-title":"Warming delays ovarian development in a capital breeder","volume":"164","author":"Wright","year":"2017","journal-title":"Mar. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1111\/eva.13187","article-title":"Sex- and time-specific parental effects of warming on reproduction and offspring quality in a coral reef fish","volume":"14","author":"Spinks","year":"2021","journal-title":"Evol. Appl."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.mce.2013.04.014","article-title":"Steroid hormone synthesis in mitochondria","volume":"379","author":"Miller","year":"2013","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.tem.2017.09.002","article-title":"Steroidogenesis: Unanswered questions","volume":"28","author":"Miller","year":"2017","journal-title":"Trends Endocrin. Met."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liu, W., Gong, T., Shi, F., Xu, H., and Chen, X. (2022). Taste receptors affect male reproduction by influencing steroid synthesis. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.956981"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Shah, W., Khan, R., Shah, B., Khan, A., Dil, S., and Liu, W. (2021). The molecular mechanism of sex hormones on sertoli cell development and proliferation. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.648141"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Detlefsen, A., Paulukinas, R., and Penning, T. (2023). Germline mutations in steroid metabolizing enzymes: A focus on steroid transforming aldo-keto reductases. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24031873"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/s41419-023-05864-3","article-title":"Autophagy regulates sex steroid hormone synthesis through lysosomal degradation of lipid droplets in human ovary and testis","volume":"14","author":"Esmaeilian","year":"2023","journal-title":"Cell Death Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Wu, J., Li, X., Chen, Q., Hong, Z., Zheng, L., Huang, S., Mo, P., Li, C., and Wang, R. (2024). Protective effect of Huangqi-Guizhi-Wuwutang against cyclophosphamide-induced spermatogenesis dysfunction in mice by promoting steroid hormone biosynthesis. J. Ethnopharmacol., 319.","DOI":"10.1016\/j.jep.2023.117260"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.mce.2003.11.024","article-title":"Function of Cyp11a1 in animal models","volume":"215","author":"Hu","year":"2004","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ikegawa, K., and Hasegawa, Y. (2023). Adrenal gland involvement in 11-ketotestosterone production analyzed using LC-MS\/MS. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1051195"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jsbmb.2013.04.010","article-title":"11\u03b2-hydroxyandrostenedione, the product of androstenedione metabolism in the adrenal, is metabolized in LNCaP cells by 5\u03b1-reductase yielding 11\u03b2-hydroxy-5\u03b1-androstanedione","volume":"138","author":"Swart","year":"2013","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Li, Y., Zhao, L., Luo, R., Xu, G., Fang, Y., Wang, B., and Chen, J. (2024). Development of gonad and brood pouch and expression profiles of cg\u03b1, fsh\u03b2 and lh\u03b2 transcripts and plasma 17\u03b2-E2 and 11-KT during the first reproductive cycle of farmed big-belly seahorse (Hippocampus abdominalis). Aquaculture, 589.","DOI":"10.1016\/j.aquaculture.2024.740986"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1530\/JOE-19-0438","article-title":"Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone","volume":"244","author":"Zheng","year":"2020","journal-title":"J. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ygcen.2007.02.009","article-title":"Non-invasive measurement of 11-ketotestosterone, cortisol and androstenedione in male three-spined stickleback (Gasterosteus aculeatus)","volume":"152","author":"Sebire","year":"2007","journal-title":"Gen. Comp. Endocr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1210\/en.2016-1865","article-title":"Targeted disruption of aromatase reveals dual functions of cyp19a1a during sex differentiation in zebrafish","volume":"158","author":"Yin","year":"2017","journal-title":"Endocrinology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.cell.2019.10.005","article-title":"Cellular Senescence: Defining a Path Forward","volume":"179","author":"Gorgoulis","year":"2019","journal-title":"Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/s41598-020-57839-5","article-title":"Differential expression and localisation of TGF-beta isoforms and receptors in the murine epididymis","volume":"10","author":"Voisin","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1210\/er.2002-0003","article-title":"Genetic analysis of the mammalian transforming growth factor-beta superfamily","volume":"23","author":"Chang","year":"2002","journal-title":"Endoc. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.3349\/ymj.2015.56.6.1590","article-title":"The role of Foxo3 in Leydig cells","volume":"56","author":"Choi","year":"2015","journal-title":"Yonsei Med. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1093\/biolre\/ioad168","article-title":"BNC1 deficiency induces mitochondrial dysfunction-triggered spermatogonia apoptosis through the CREB\/SIRT1\/FOXO3 pathway: The therapeutic potential of nicotinamide riboside and metformin","volume":"110","author":"Ni","year":"2024","journal-title":"Biol. Reprod."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Crapster, J., Rack, P., Hellmann, Z., Le, A., Adams, C., Leib, R., Elias, J., Perrino, J., Behr, B., and Li, Y. (2020). HIPK4 is essential for murine spermiogenesis. eLife, 9.","DOI":"10.7554\/eLife.50209"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Dong, M., Tang, M., Li, W., Li, S., Yi, M., and Liu, W. (2023). Morphological and transcriptional analysis of sexual differentiation and gonadal development in a burrowing fish, the four-eyed sleeper (Bostrychus sinensis). Comp. Biochem. Phys. D, 48.","DOI":"10.1016\/j.cbd.2023.101148"},{"key":"ref_52","first-page":"44","article-title":"Testis of Male Tilapia Under Methomyl Stress: Transcriptome Changes and Signal Pathway Analysis","volume":"35","author":"Meng","year":"2019","journal-title":"Chin. Agric. Sci. Bull."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Luo, D., He, Z., Yu, C., and Guan, Q. (2022). Role of p38 MAPK Signalling in Testis Development and Male Fertility. Oxidative Med. Cell. Longev., 2022.","DOI":"10.1155\/2022\/6891897"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kumar, L., Solanki, S., Jain, A., Botts, M., Gupta, R., Rajput, S., and Roti Roti, E. (2024). MAPKs signaling is obligatory for male reproductive function in a development-specific manner. Front. Reprod. Health, 6.","DOI":"10.3389\/frph.2024.1330161"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1002\/tox.23808","article-title":"Integrated metabolomics and transcriptomics reveal glyphosate based-herbicide induced reproductive toxicity through disturbing energy and nucleotide metabolism in mice testes","volume":"38","author":"Qi","year":"2023","journal-title":"Environ. Toxicol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s00432-002-0413-7","article-title":"Cloning and functional characterization of GMPR2, a novel human guanosine monophosphate reductase, which promotes the monocytic differentiation of HL-60 leukemia cells","volume":"129","author":"Zhang","year":"2003","journal-title":"J. Cancer Res. Clin."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/0022-4731(90)90179-V","article-title":"Ethanol-induced inhibition of testosterone biosynthesis in rat Leydig cells: Central role of mitochondrial NADH redox state","volume":"36","author":"Orpana","year":"1990","journal-title":"J. Steroid Biochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1530-0277.1983.tb05426.x","article-title":"Inhibition of testosterone production by rat Leydig cells with ethanol and acetaldehyde: Prevention of ethanol toxicity with 4-methylpyrazole","volume":"7","author":"Santucci","year":"1983","journal-title":"Clin. Exp. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s12032-018-1144-1","article-title":"A review of HPRT and its emerging role in cancer","volume":"35","author":"Townsend","year":"2018","journal-title":"Med. Oncol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"M\u00e1ty\u00e1si, B., Pet\u0151v\u00e1ri, G., Dank\u00f3, T., Butz, H., Lik\u00f3, I., L\u0151w, P., Petit, I., Bittar, R., Bonnefont-Rousselot, D., and Farkas, Z. (2022). Extracellular vesicle-mediated metastasis suppressors NME1 and NME2 modify lipid metabolism in fibroblasts. Cancers, 14.","DOI":"10.3390\/cancers14163913"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"97A","DOI":"10.1016\/j.bpj.2022.11.718","article-title":"Knock-out of mitochondrial nucleoside diphosphate kinase NME4 reveals surprising tissue-distribution and remodeled cell signaling, suggestive of multiple functions","volume":"122","author":"Rstom","year":"2023","journal-title":"Biophys. J."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/2\/327\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:20:19Z","timestamp":1769145619000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/2\/327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,21]]},"references-count":61,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ani16020327"],"URL":"https:\/\/doi.org\/10.3390\/ani16020327","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,21]]}}}