{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T16:21:32Z","timestamp":1769703692479,"version":"3.49.0"},"reference-count":21,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019014","name":"Chengdu Science and Technology Program","doi-asserted-by":"publisher","award":["2023YF09-00017-SN"],"award-info":[{"award-number":["2023YF09-00017-SN"]}],"id":[{"id":"10.13039\/501100019014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020145","name":"Chengdu Research Base of Giant Panda Breeding","doi-asserted-by":"crossref","award":["2024CPB-C09"],"award-info":[{"award-number":["2024CPB-C09"]}],"id":[{"id":"10.13039\/100020145","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100020145","name":"Chengdu Research Base of Giant Panda Breeding","doi-asserted-by":"crossref","award":["2024CPB-C10"],"award-info":[{"award-number":["2024CPB-C10"]}],"id":[{"id":"10.13039\/100020145","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Toxoplasma gondii is a globally distributed zoonotic parasite that infects a wide range of warm-blooded animals and poses serious threats to many endangered species. Effective serological detection is critical for disease surveillance and control, yet the accuracy of commercial assays varies significantly across host species. Validated serological methods for the Chinese red panda (Ailurus styani) remain scarce, limiting effective monitoring and conservation efforts. A total of 57 serum samples were collected from captive red pandas. Three commercial serological kits\u2014the modified agglutination test (MAT), indirect hemagglutination assay (IHA), and enzyme-linked immunosorbent assay (ELISA)\u2014were used to detect T. gondii antibodies. Statistical analyses included agreement assessment (Cohen\u2019s kappa), McNemar\u2019s test, and receiver operating characteristic (ROC) curve analysis, with MAT as the reference standard. MAT detected T. gondii antibodies in 36.84% (21\/57; 95% CI: 24.32\u201349.36) of samples, while ELISA identified 42.11% (24\/57; 95% CI: 29.29\u201354.92). IHA failed to detect any positive samples (0%). MAT and ELISA showed almost perfect agreement (\u03ba = 0.817; 95% CI: 0.66\u20130.97) with no significant difference (p &gt; 0.05). Compared to MAT, ELISA exhibited 95.2% sensitivity and 88.9% specificity. The combined MAT\/ELISA approach with retesting of discordant samples yielded a final seroprevalence of 35.09% (20\/57), with 8.77% (5\/57) indeterminate results. MAT and ELISA are reliable methods for serological surveillance of T. gondii in red pandas, while IHA is unsuitable. We recommend MAT for initial screening followed by ELISA confirmation. The high seroprevalence highlights the need for integrated control strategies, including management of stray felids and rodents in captive environments to reduce transmission risks.<\/jats:p>","DOI":"10.3390\/ani16030396","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:41:56Z","timestamp":1769528516000},"page":"396","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Serological Detection Methods for Toxoplasma gondii Antibodies and Seroprevalence in Captive Red Pandas"],"prefix":"10.3390","volume":"16","author":[{"given":"Chanjuan","family":"Yue","sequence":"first","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanjing","family":"Yang","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dunwu","family":"Qi","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanshan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyang","family":"Fan","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Chen","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Wen","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolan","family":"Wang","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunli","family":"Li","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Hou","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7311-9286","authenticated-orcid":false,"given":"Songrui","family":"Liu","sequence":"additional","affiliation":[{"name":"Chengdu Research Base of Giant Panda Breeding, The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, 1375 Panda Road, Chenghua District, Chengdu 610081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"eaax5751","DOI":"10.1126\/sciadv.aax5751","article-title":"Genomic evidence for two phylogenetic species and long-term population bottlenecks in red pandas","volume":"6","author":"Hu","year":"2020","journal-title":"Sci. Adv."},{"key":"ref_2","unstructured":"Glatston, A.R. (2021). Red pandas in the wild in China. Red Panda, Academic Press. [2nd ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Liu, S., Li, Y., Zhang, D., Su, X., Yue, C., Ayala, J.E., Yan, X., Hou, R., Li, L., and Xie, Y. (2022). Mortality analysis of captive red panda cubs within Chengdu, China. BMC Vet. Res., 18.","DOI":"10.1186\/s12917-022-03170-2"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Dubey, J.P. (2022). Toxoplasmosis of Animals and Humans, CRC Press. [3rd ed.].","DOI":"10.1201\/9781003199373"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ijppaw.2013.02.002","article-title":"Wildlife disease ecology in changing landscapes: Mesopredator release and toxoplasmosis","volume":"2","author":"Hollings","year":"2013","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1089\/vbz.2020.2658","article-title":"Prevalence and risk factors of Toxoplasma gondii infection among five wild rodent species from five provinces of China","volume":"21","author":"Lv","year":"2021","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/zph.12122","article-title":"Comparison of different commercial serological tests for the detection of Toxoplasma gondii antibodies in serum of naturally exposed pigs","volume":"62","author":"Steinparzer","year":"2015","journal-title":"Zoonoses Public Health"},{"key":"ref_8","unstructured":"Chen, R.F. (2015). Investigation and Genotyping of Toxoplasma gondii for Bred Wild Animals in Fuzhou. [Master\u2019s Thesis, Fujian Agriculture and Forestry University]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1638\/06-048.1","article-title":"Serosurvey of infectious disease agents of carnivores in captive red pandas (Ailurus fulgens) in China","volume":"38","author":"Qin","year":"2007","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S1383-5769(00)00041-6","article-title":"Prevalence of antibodies to Toxoplasma gondii in the sera of rare wildlife in the Shanghai Zoological Garden, People\u2019s Republic of China","volume":"49","author":"Zhang","year":"2000","journal-title":"Parasitol. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.vetpar.2005.11.021","article-title":"Seroprevalences of antibodies to Neospora caninum and Toxoplasma gondii in zoo animals","volume":"136","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ijppaw.2019.02.006","article-title":"Evidence of red panda as an intermediate host of Toxoplasma gondii and Sarcocystis species","volume":"8","author":"Yang","year":"2019","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1055\/s-0038-1623622","article-title":"Zwei Fallberichte einer disseminierten Toxoplasmose [Two cases of disseminated toxoplasmosis]","volume":"40","author":"Stief","year":"2012","journal-title":"Tierarztl. Prax. Ausg. K Kleintiere Heimtiere"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1638\/2019-0026","article-title":"Clinicopathological features of toxoplasmosis in four red pandas (Ailurus fulgens)","volume":"51","author":"Ashley","year":"2020","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109145","DOI":"10.1016\/j.vetpar.2020.109145","article-title":"All about toxoplasmosis in cats: The last decade","volume":"283","author":"Dubey","year":"2020","journal-title":"Vet. Parasitol."},{"key":"ref_16","first-page":"89","article-title":"Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma gondii antibodies in sera of naturally infected dogs and cats","volume":"7","author":"Zhu","year":"2012","journal-title":"Iran. J. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.ijppaw.2022.07.001","article-title":"Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca)","volume":"18","author":"Yue","year":"2022","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1939-1676.1991.tb03137.x","article-title":"Comparison of latex agglutination, indirect hemagglutination, and ELISA techniques for the detection of Toxoplasma gondii-specific antibodies in the serum of cats","volume":"5","author":"Lappin","year":"1991","journal-title":"J. Vet. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1638\/2006-0008R.1","article-title":"Serosurvey of ex-situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation","volume":"38","author":"Loeffler","year":"2007","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2307\/3284018","article-title":"Prevalence of viable Toxoplasma gondii tissue cysts and antibodies to T. gondii by various serologic tests in black bears (Ursus americanus) from Pennsylvania","volume":"81","author":"Dubey","year":"1995","journal-title":"J. Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yue, C., Yang, W., Fan, X., Lan, J., Huang, W., Zhang, D., Li, Y., Liao, L., Ayala, J.E., and Wu, K. (2022). Seroprevalence and risk factors of Toxoplasma gondii infection in captive giant panda (Ailuropoda melanoleuca). Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.1071988"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/3\/396\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T05:11:57Z","timestamp":1769663517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/3\/396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ani16030396"],"URL":"https:\/\/doi.org\/10.3390\/ani16030396","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}