{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T07:47:16Z","timestamp":1770364036856,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300347"],"award-info":[{"award-number":["32300347"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Scientific Research Project in University of Henan Province","award":["25A240002"],"award-info":[{"award-number":["25A240002"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3205903"],"award-info":[{"award-number":["2023YFC3205903"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Toll-like receptors (TLRs) are essential pattern recognition receptors of the innate immune system and play critical roles in pathogen invasion in teleosts. In this study, we identified and characterized full-length open reading frames of three TLRs belonging to the TLR11 subfamily from Schizothorax prenanti, termed spTLR19 (2868 bp), spTLR20 (2835 bp), and spTLR21 (2946 bp), encoding 955, 944, and 981 amino acids, respectively. All three proteins exhibited the conserved domain architecture typical of TLRs, comprising a leucine-rich repeat (LRR) domain, a transmembrane region, and a Toll\/IL-1 receptor (TIR) domain. Phylogenetic and homology analyses revealed that spTLR19 and spTLR20 clustered most closely with their homologues from Cyprinus carpio, while spTLR21 showed the highest similarity to Onychostoma macrolepis TLR21. Expression profiling showed that these TLRs were ubiquitously expressed across examined tissues, with relatively higher expression in immune-related tissues such as spleen and gills. Furthermore, challenge with Streptococcus agalactiae and Aeromonas veronii significantly up-regulated the expression of spTLR19, spTLR20, and spTLR21 in spleen, liver, and gills, suggesting their involvement in antibacterial immune responses. These findings enhance the functional understanding of the teleost TLR11 subfamily and provide a foundation for elucidating disease resistance and immune regulation in S. prenanti.<\/jats:p>","DOI":"10.3390\/ani16030511","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T16:30:47Z","timestamp":1770309047000},"page":"511","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Cloning and Expression Responses to Streptococcus agalactiae and Aeromonas veronii of TLR19, TLR20, and TLR21 in Schizothorax prenanti"],"prefix":"10.3390","volume":"16","author":[{"given":"Qiyu","family":"Luo","sequence":"first","affiliation":[{"name":"Henan Province Engineering Research Center of Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Province Engineering Research Center of Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Shi","sequence":"additional","affiliation":[{"name":"Henan Province Engineering Research Center of Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Henan Province Engineering Research Center of Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3738-2711","authenticated-orcid":false,"given":"Yuanchao","family":"Zou","sequence":"additional","affiliation":[{"name":"Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Life Sciences, Neijiang Normal University, Neijiang 641100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghui","family":"Kong","sequence":"additional","affiliation":[{"name":"Henan Province Engineering Research Center of Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.17221\/3294-VETMED","article-title":"Innate and Adaptive Immunity in Teleost Fish: A Review","volume":"56","author":"Uribe","year":"2011","journal-title":"Vet. 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