{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:30:24Z","timestamp":1780403424910,"version":"3.54.1"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010699","name":"Monash University Malaysia","doi-asserted-by":"crossref","award":["ASEAN-2019-04-MED"],"award-info":[{"award-number":["ASEAN-2019-04-MED"]}],"id":[{"id":"10.13039\/501100010699","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Southeast Asia Biodiversity Research Institute, Chinese Academy of Science","award":["Y4ZK111B01"],"award-info":[{"award-number":["Y4ZK111B01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Understanding patterns of genetic diversity and population structure in freshwater fishes is crucial for evolutionary studies and conservation planning. Tor tambra is a common freshwater fish species in Thai waters, yet its genetic diversity and population connectivity remain largely unexplored. This study investigated the phylogeographic structure and genetic diversity of Thai T. tambra through phylogenetic analyses and molecular approaches using mitochondrial DNA markers (COI and Cytb). Our analyses revealed four distinct genetic lineages (clades A-D) within Thai T. tambra. Lineage C comprised specimens from southern Thailand, Malaysia, Indonesia, and southern China, while lineages A, B, and D were distributed exclusively within Thailand. These Thai-endemic lineages exhibited pronounced geographic structuring across northern and western regions, with lineage A widespread, lineage B confined to northern areas, and lineage D restricted to western localities. The genetic differentiation and distribution patterns of these lineages suggest influence from the limited north\u2013south river system connections and the biogeographic barrier posed by the Isthmus of Kra. Multiple lineages co-occurred at some locations, indicating a complex evolutionary history. These findings provide important implications for understanding the evolutionary processes and biogeographic factors shaping genetic diversity within T. tambra across the riverine systems of Thailand.<\/jats:p>","DOI":"10.3390\/ani16030517","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T13:33:50Z","timestamp":1770384830000},"page":"517","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phylogeographic Structure Reveals Hidden Diversity Patterns in Tor tambra (Cyprinidae) Across Thai River Systems"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6977-1051","authenticated-orcid":false,"given":"Vatthanachai","family":"Phanklam","sequence":"first","affiliation":[{"name":"Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sommai","family":"Janekitkarn","sequence":"additional","affiliation":[{"name":"Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3601-1638","authenticated-orcid":false,"given":"Kathathep","family":"Seesan","sequence":"additional","affiliation":[{"name":"Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7954-7348","authenticated-orcid":false,"given":"Narongrit","family":"Muangmai","sequence":"additional","affiliation":[{"name":"Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Biodiversity Center, Kasetsart University (BDCKU), Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1003-9382","authenticated-orcid":false,"given":"Cong","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Oceanography, Shanghai Jiao Tong University, Shanghai 200030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0354-6538","authenticated-orcid":false,"given":"Prapansak","family":"Srisapoome","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5985-7258","authenticated-orcid":false,"given":"Kornsorn","family":"Srikulnath","sequence":"additional","affiliation":[{"name":"Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5700-1616","authenticated-orcid":false,"given":"Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin 05282, Myanmar"},{"name":"Yunnan Key Laboratory of Biodiversity and Ecological Conservation of Gaoligong Mountain, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0924-5560","authenticated-orcid":false,"given":"Xiao-Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin 05282, Myanmar"},{"name":"Yunnan Key Laboratory of Biodiversity and Ecological Conservation of Gaoligong Mountain, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0804-3561","authenticated-orcid":false,"given":"Uthairat","family":"Na-Nakorn","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishwar S.","family":"Parhar","sequence":"additional","affiliation":[{"name":"Brain Research Institute Monash Sunway (BRIMS), Jeffrey Cheah School of Medicine and Health Science, Monash University Malaysia, Bandar Sunway 47500, Malaysia"},{"name":"Center Initiative for Training International Researchers, University of Toyama, Toyama 930-0194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Closs, G.P., Krkosek, M., and Olden, J.D. (2016). Conservation of Freshwater Fishes, Cambridge University Press.","DOI":"10.1017\/CBO9781139627085"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1002\/aqc.2740","article-title":"Heads you win, tails you lose: Life-history traits predict invasion and extinction risk of the world\u2019s freshwater fishes","volume":"27","author":"Liu","year":"2017","journal-title":"Aquat. Conserv."},{"key":"ref_3","first-page":"94","article-title":"Morphometric and genetic divergence of hatchery populations of walking catfish, Clarias macrocephalus","volume":"252","author":"Kamonrat","year":"2006","journal-title":"Aquaculture"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.ympev.2016.08.014","article-title":"Molecular phylogeny and historical biogeography of the Indonesian freshwater fish Rasbora lateristriata species complex (Actinopterygii: Cyprinidae): Cryptic species and west-to-east divergences","volume":"105","author":"Kusuma","year":"2016","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"145708","DOI":"10.1016\/j.gene.2021.145708","article-title":"Improving the phylogenetic resolution of Malaysian and Javan mahseer (Cyprinidae), Tor tambroides and Tor tambra: Whole mitogenomes sequencing, phylogeny and potential mitogenome markers","volume":"791","author":"Lim","year":"2021","journal-title":"Gene"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"301","DOI":"10.2306\/scienceasia1513-1874.2019.45.301","article-title":"Genetic diversity of the North African catfish, Clarias gariepinus (Burchell, 1822) hatchery stocks in Thailand","volume":"45","author":"Wachirachaikarn","year":"2019","journal-title":"ScienceAsia"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chew, P.C., Christianus, A., Zudaidy, J.M., Ina-Salwany, M.Y., Chong, C.M., and Tan, S.G. (2021). Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization. Animals, 11.","DOI":"10.3390\/ani11092633"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wattanadilokchatkun, P., Panthum, T., Jaisamut, K., Ahmad, S.F., Dokkaew, S., Muangmai, N., Duengkae, P., Singchat, W., and Srikulnath, K. (2022). Characterization of microsatellite distribution in siamese fighting fish genome to promote conservation and genetic diversity. Fishes, 7.","DOI":"10.3390\/fishes7050251"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1038\/sj.hdy.6800613","article-title":"Phylogeographic evidence for the existence of an ancient biogeographic barrier: The Isthmus of Kra Seaway","volume":"94","author":"Nugroho","year":"2005","journal-title":"Heredity"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1756-1051.2012.00121.x","article-title":"The biogeography of the Isthmus of Kra region: A review","volume":"31","author":"Parnell","year":"2013","journal-title":"Nord. J. Bot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"106806","DOI":"10.1016\/j.ympev.2020.106806","article-title":"Sea water shaping the freshwater biota: Hidden diversity and biogeographic history in the Paracanthocobitis zonalternans species complex (Teleostei: Nemacheilidae) in western Southeast Asia","volume":"148","author":"Bohlen","year":"2020","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.aquaculture.2004.08.001","article-title":"Genetic diversity of walking catfish, Clarias macrocephalus, in Thailand and evidence of genetic introgression from introduced farmed C. gariepinus","volume":"240","author":"Kamonrat","year":"2004","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gower, D.J., Johnson, K., Richardson, J., Rosen, B., R\u00fcber, L., and Williams, S. (2012). Molecular biogeography and phylogeography of the freshwater fauna of the Indo-Australian Archipelago. Biotic Evolution and Environmental Change in Southeast Asia, Cambridge University Press.","DOI":"10.1017\/CBO9780511735882"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.ympev.2010.03.027","article-title":"A reappraisal of the evolution of Asian snakehead fishes (Pisces, Channidae) using molecular data from multiple genes and fossil calibration","volume":"56","author":"Adamson","year":"2010","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Beck, S.V., Carvalho, G.R., Barlow, A., R\u00fcber, L., Hui Tan, H., Nugroho, E., Wowor, D., Mohd Nor, S.A., Herder, F., and Muchlisin, Z.A. (2017). Plio-Pleistocene phylogeography of the Southeast Asian Blue Panchax killifish, Aplocheilus panchax. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0179557"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s10531-010-9783-3","article-title":"Biogeography and conservation in Southeast Asia: How 2.7 million years of repeated environmental fluctuations affect today\u2019s patterns and the future of the remaining refugial-phase biodiversity","volume":"19","author":"Woodruff","year":"2010","journal-title":"Biodivers. Conserv."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.jop.2024.10.001","article-title":"Palaeodrainages of the Sunda Shelf detailed in new maps","volume":"14","author":"Cheng","year":"2025","journal-title":"J. Palaeogeogr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1046\/j.1365-2699.2000.00489.x","article-title":"Maps of Pleistocene sea levels in Southeast Asia: Shorelines, river systems and time durations","volume":"27","author":"Voris","year":"2000","journal-title":"J. Biogeogr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1471-8286.2006.01546.x","article-title":"Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners","volume":"7","author":"Nguyen","year":"2006","journal-title":"Mol. Ecol. Notes"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s11160-019-09566-y","article-title":"Mahseer (Tor spp.) fishes of the world: Status, challenges and opportunities for conservation","volume":"29","author":"Pinder","year":"2019","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Jaafar, F., Na-Nakorn, U., Srisapoome, P., Amornsakun, T., Duong, T.Y., Gonzales-Plasus, M.M., Hoang, D.H., and Parhar, I.S. (2021). A current update on the distribution, morphological features, and genetic identity of the Southeast Asian mahseers, Tor species. Biology, 10.","DOI":"10.3390\/biology10040286"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5662867","DOI":"10.1155\/2024\/5662867","article-title":"Molecular systematics and divergence of Tor and Neolissochilus fishes (Cypriniforms: Cyprinidae) from Southeast Asia and South China","volume":"2024","author":"Wu","year":"2024","journal-title":"J. Zool. Syst. Evol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1111\/j.1095-8649.2011.03086.x","article-title":"Asian river fishes in the Anthropocene: Threats and conservation challenges in an era of rapid environmental change","volume":"79","author":"Dudgeon","year":"2011","journal-title":"J. Fish Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"170980","DOI":"10.1155\/2013\/170980","article-title":"Genetic structure and preliminary findings of cryptic diversity of the Malaysian Mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses","volume":"2013","author":"Esa","year":"2013","journal-title":"Biomed Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"551","DOI":"10.11646\/zootaxa.4006.3.8","article-title":"Mahseers genera Tor and Neolissochilus (Teleostei: Cyprinidae) from southern Vietnam","volume":"4006","author":"Hoang","year":"2015","journal-title":"Zootaxa"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/23308249.2016.1251391","article-title":"Disentangling the taxonomy of the mahseers (Tor spp.) of Malaysia: An integrated approach using morphology, genetics and historical records","volume":"25","author":"Walton","year":"2017","journal-title":"Rev. Fish. Sci. Aquac."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1016\/j.csbj.2022.03.021","article-title":"The first de novo genome assembly and sex marker identification of Pluang Chomphu fish (Tor tambra) from Southern Thailand","volume":"20","author":"Surachat","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_28","unstructured":"Kottelat, M. (2013). The fishes of the inland waters of Southeast Asia: A catalogue and core bibliography of the fishes known to occur in freshwaters, mangroves and estuaries. Raffles Bull. Zool., 1\u2013663."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1098\/rstb.2005.1716","article-title":"DNA barcoding Australia\u2019s fish species","volume":"360","author":"Ward","year":"2005","journal-title":"Philos. Trans. R. Soc. B"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ympev.2010.05.021","article-title":"Systematics of the subfamily Danioninae (Teleostei: Cypriniformes: Cyprinidae)","volume":"57","author":"Tang","year":"2010","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: Fast model selection for accurate phylogenetic estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies: An approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1111\/j.1755-0998.2011.03021.x","article-title":"Kakusan4 and Aminosan: Two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data","volume":"11","author":"Tanabe","year":"2011","journal-title":"Mol. Ecol. Resour."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"POPART: Full-feature software for haplotype network construction","volume":"6","author":"Leigh","year":"2015","journal-title":"Methods Ecol. Evol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"10602","DOI":"10.1073\/pnas.1016437108","article-title":"Genetic calibration of species diversity among North America\u2019s freshwater fishes","volume":"108","author":"April","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1111\/j.1755-0998.2009.02541.x","article-title":"DNA barcode divergence among species and genera of birds and fishes","volume":"9","author":"Ward","year":"2009","journal-title":"Mol. Ecol. Resour."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Pereira, L.H.G., Hanner, R., Foresti, F., and Oliveira, C. (2013). Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna?. BMC Genet., 14.","DOI":"10.1186\/1471-2156-14-20"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Laskar, B.A., Bhattacharjee, M.J., Dhar, B., Mahadani, P., Kundu, S., and Ghosh, S.K. (2013). The species dilemma of northeast Indian mahseer (Actinopterygii: Cyprinidae): DNA barcoding in clarifying the riddle. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0053704"},{"key":"ref_40","first-page":"263","article-title":"Molecular systematics of mahseers (Cyprinidae) in Malaysia inferred from sequencing of a mitochondrial cytochrome c oxidase I (COI) gene","volume":"31","author":"Esa","year":"2008","journal-title":"Pertanika J. Trop. Agric. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3897\/zookeys.746.13502","article-title":"Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)","volume":"746","author":"Adeoba","year":"2018","journal-title":"ZooKeys"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1752-4571.2009.00110.x","article-title":"Species-specific responses to landscape fragmentation: Implications for management strategies","volume":"3","author":"Blanchet","year":"2010","journal-title":"Evol. Appl."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.tree.2012.05.012","article-title":"Harnessing genomics for delineating conservation units","volume":"27","author":"Funk","year":"2012","journal-title":"Trends Ecol. Evol."},{"key":"ref_44","first-page":"1","article-title":"Maps of Holocene sea level transgression and submerged lakes on the Sunda Shelf","volume":"6","author":"Sathiamurthy","year":"2006","journal-title":"Nat. Hist. J. Chulalongkorn Univ."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1365-2117.2006.00285.x","article-title":"Subsidence in the super-deep Pattani and Malay basins of Southeast Asia: A coupled model incorporating lower-crustal flow in response to post-rift sediment loading","volume":"18","author":"Morley","year":"2006","journal-title":"Basin Res."},{"key":"ref_46","first-page":"1","article-title":"Phylogeny and phylogeography of Barbonymus schwanenfeldii (Cyprinidae) from Malaysia inferred using partial cytochrome b mtDNA gene","volume":"5","author":"Kamaruddin","year":"2009","journal-title":"J. Trop. Biol. Conserv."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"11188","DOI":"10.1073\/pnas.0809865106","article-title":"The current refugial rainforests of Sundaland are unrepresentative of their biogeographic past and highly vulnerable to disturbance","volume":"106","author":"Cannon","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/jbi.12416","article-title":"Shifting seas: The impacts of Pleistocene sea-level fluctuations on the evolution of tropical marine taxa","volume":"42","author":"Ludt","year":"2015","journal-title":"J. Biogeogr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"893","DOI":"10.2108\/zsj.23.893","article-title":"Phylogenetic analysis of Hampala fishes (Subfamily Cyprininae) in Malaysia inferred from partial mitochondrial cytochrome b DNA sequences","volume":"23","author":"Ryan","year":"2006","journal-title":"Zool. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tan, M.P., Jamsari, A.F.J., and Siti Azizah, M.N. (2013). Phylogeographic pattern of the striped snakehead, Channa striata in Sundaland: Ancient river connectivity, geographical and anthropogenic signatures. PLoS ONE, 8.","DOI":"10.1371\/annotation\/2be4a0a9-b3ed-492f-8c4d-15d3a5ae6cab"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"48","DOI":"10.5670\/oceanog.2019.119","article-title":"Blowing in the monsoon wind","volume":"32","author":"Wang","year":"2019","journal-title":"Oceanography"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/j.1365-294X.1995.tb00226.x","article-title":"Phylogeographic structure in mitochondrial DNA of a South-east Asian freshwater fish, Hemibagrus nemurus (Siluroidei; Bagridae) and Pleistocene sea-level changes on the Sunda shelf","volume":"4","author":"Dodson","year":"1995","journal-title":"Mol. Ecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1017\/S1464793105006950","article-title":"Freshwater biodiversity: Importance, threats, status and conservation challenges","volume":"81","author":"Dudgeon","year":"2006","journal-title":"Biol. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1080\/10635150252899752","article-title":"Strategies to protect biological diversity and the evolutionary processes that sustain it","volume":"51","author":"Moritz","year":"2002","journal-title":"Syst. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Yadav, P., Kumar, A., Hussain, S.A., and Gupta, S.K. (2020). Evaluation of the effect of longitudinal connectivity in population genetic structure of endangered golden mahseer, Tor putitora (Cyprinidae), in Himalayan rivers: Implications for its conservation. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0234377"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3841","DOI":"10.1007\/s10528-023-10623-2","article-title":"High genetic differentiation and genetic diversity in endangered mahseer Tor khudree (Sykes, 1839) as revealed from concatenated ATPase 6\/8 and Cyt b mitochondrial genes","volume":"62","author":"Das","year":"2024","journal-title":"Biochem. Genet."},{"key":"ref_57","first-page":"125","article-title":"Life-history traits and decadal trends in the growth parameters of Golden mahseer Tor putitora (Hamilton 1822) from the Himalayan stretch of the Ganga River System","volume":"8","author":"Nautiyal","year":"2008","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1080\/09712119.2018.1497493","article-title":"Pattern of reproductive biology of the endangered golden mahseer Tor putitora (Hamilton 1822) with special reference to regional climate change implications on breeding phenology from lesser Himalayan region, India","volume":"46","author":"Joshi","year":"2018","journal-title":"J. Appl. Anim. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"8971","DOI":"10.1038\/s41598-023-36065-9","article-title":"Year-round spawning of three tropical Cypriniformes fishes in Southeast Asia","volume":"13","author":"Kahar","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1002\/aqc.2712","article-title":"Fish conservation in freshwater and marine realms: Status, threats and management","volume":"26","author":"Arthington","year":"2016","journal-title":"Aquat. Conserv."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/3\/517\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T05:23:19Z","timestamp":1770528199000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/3\/517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,6]]},"references-count":60,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ani16030517"],"URL":"https:\/\/doi.org\/10.3390\/ani16030517","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,6]]}}}