{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T20:44:18Z","timestamp":1780692258285,"version":"3.54.1"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Provincial Rural Revitalization Strategy","award":["2024-SPY-00\u2013016"],"award-info":[{"award-number":["2024-SPY-00\u2013016"]}]},{"name":"Science and Technology Program of Guangdong Province","award":["2019B030316022"],"award-info":[{"award-number":["2019B030316022"]}]},{"name":"China-ASEAN Maritime Cooperation Fund","award":["CAMC-2018F"],"award-info":[{"award-number":["CAMC-2018F"]}]},{"DOI":"10.13039\/501100014881","name":"Guangzhou University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014881","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The tire track eel (Mastacembelus favus) is a freshwater fish with high economic value and aquaculture potential. However, its sex determination mechanism remains unclear, which limits the development of monosex culture and sex-controlled breeding. To address this, preliminary female and male reference genomes were generated using second-generation sequencing, followed by whole-genome resequencing of four females and four males. Comparative analyses identified 69 male-specific sequences, with a total length of approximately 44.5 kb. Based on these sequences, two PCR-based sex-specific markers (W5 and W14) were developed. Both markers showed complete concordance with phenotypic sex in parental and offspring from controlled crosses populations, providing strong evidence for a male heterogametic (XY) sex determination system in M. favus. Although the markers were not transferable to the closely related species Mastacembelus armatus, the corresponding male-specific sequences exhibited high genomic conservation. In conclusion, this study provides reliable molecular tools for genetic sex identification in M. favus. These tools will support monosex aquaculture and sex-controlled breeding programs, while also offering insights into sex determination and sex chromosome evolution in the genus Mastacembelus.<\/jats:p>","DOI":"10.3390\/ani16040605","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T14:09:54Z","timestamp":1771250994000},"page":"605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sex-Specific Marker Development and Identification of an XY Sex Determination System in the Tire Track Eel (Mastacembelus favus) via Whole-Genome Resequencing"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-8649","authenticated-orcid":false,"given":"Linan","family":"Zhang","sequence":"first","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiman","family":"Chen","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshi","family":"Zhu","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Sheng","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikai","family":"Chen","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Li","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxiang","family":"Cui","sequence":"additional","affiliation":[{"name":"Guangdong Lianyi Aquatic Science and Technology Development Co., Ltd., Kaiping 529300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0822-508X","authenticated-orcid":false,"given":"Chong","family":"Han","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Shu","sequence":"additional","affiliation":[{"name":"South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s11434-012-5577-1","article-title":"Progress in studies of fish reproductive development regulation","volume":"58","author":"Chen","year":"2013","journal-title":"Chin. 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