{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:38:10Z","timestamp":1788961090982,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Afyon Kocatepe University Scientific Research Coordination Unit","award":["24. VF. 03"],"award-info":[{"award-number":["24. VF. 03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated the effects of dietary Myrtus communis L. extract on the production performance, immune functions, and metabolic and health status of Holstein cows throughout the transition period. Forty cows were assigned to either a control group (n = 20) or a treatment group (n = 20) receiving Myrtus communis L. extract at 1.5 mL\/kg body weight per day. Milk yield and feed intake were recorded daily, whereas blood samples were collected weekly. Reproductive performance and health status were monitored. Myrtus communis L. supplementation increased dry matter intake during prepartum and early and mid-lactation and elevated prepartum glucose levels. Treated cows produced more milk in early (44.56 vs. 40.72 kg\/day) (p &lt; 0.0001), and mid-to-late lactation (42.57 vs. 37.19 kg\/day) (p &lt; 0.0001). NEFA and BHBA concentrations were lower in the treatment group (p = 0.015 and 0.019, respectively), whereas cortisol and ALT were higher around calving (p &lt; 0.001). Despite similar prepartum inflammatory profiles, treated cows demonstrated higher TNF-\u03b1 concentrations at calving (p = 0.006). The days open and insemination number per pregnancy did not differ; however, the number of ovarian cysts were less in treated cows. Overall, Myrtus communis L. extract offered at a dose of 1.5 mL\/kg body weight during the transition period improved feed intake, enhanced milk production, and reduced metabolic stress, suggesting its potential as a natural additive to support energy balance and reproductive performance in cows. However, it increased the blood cortisol and ALT concentrations, which requires further investigation.<\/jats:p>","DOI":"10.3390\/ani16040632","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T14:55:47Z","timestamp":1771253747000},"page":"632","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Myrtus (Myrtus communis L.) Extract Supplementation in the Diet on Metabolic, Immune, and Performance Parameters of Dairy Cows During the Transition Period"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1143-1215","authenticated-orcid":false,"given":"Umit","family":"Ozcinar","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7803-2454","authenticated-orcid":false,"given":"Cangir","family":"Uyarlar","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet Emre","family":"Orman","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130brahim Sadi","family":"\u00c7eting\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0021-8730","authenticated-orcid":false,"given":"Sababa","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130smail","family":"Bayram","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, University of Afyon Kocatepe, ANS Campus, 03200 Afyonkarahisar, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s42523-025-00443-7","article-title":"The bacterial faecal microbiota shifts during the transition period in dairy cows","volume":"7","author":"Arnalot","year":"2025","journal-title":"Anim. Microbiome"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"Biology of dairy cows during the transition period: The final frontier?","volume":"82","author":"Drackley","year":"1999","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kumprechtov\u00e1, D., Chabrillat, T., Guillaume, S., Kerros, S., Kadek, R., Indrov\u00e1, E., and Illek, J. (2022). Effect of Plant Bioactive Compounds Supplemented in Transition Dairy Cows on the Metabolic and Inflammatory Status. Molecules, 27.","DOI":"10.3390\/molecules27186092"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9807","DOI":"10.3168\/jds.2023-23549","article-title":"Plant polyphenol extract supplementation affects performance, welfare, and the Nrf2-oxidative stress response in adipose tissue of heat-stressed dairy cows","volume":"106","author":"Daddam","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Barboucha, G., Rahim, N., Bramki, A., Boulebd, H., Andolfi, A., Boulacheb, K., Boulacel, A., Salvatore, M.M., and Masi, M. (2025). Comprehensive In Vitro and In Silico Analysis of Antimicrobial and Insecticidal Properties of Essential Oil of Myrtus communis L. from Algeria. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26104754"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sharifi-Rad, M., Varoni, E.M., Salehi, B., Sharifi-Rad, J., Matthews, K.R., Ayatollahi, S.A., Kobarfard, F., Ibrahim, S.A., Mnayer, D., and Zakaria, Z.A. (2017). Plants of the Genus Zingiber as a Source of Bioactive Phytochemicals: From Tradition to Pharmacy. Molecules, 22.","DOI":"10.20944\/preprints201711.0102.v1"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Khan, M.M., Lillehoj, H.S., Lee, Y., Adetunji, A.O., Omaliko, P.C., Kang, H.W., and Fasina, Y.O. (2024). Use of Selected Plant Extracts in Controlling and Neutralizing Toxins and Sporozoites Associated with Necrotic Enteritis and Coccidiosis. Appl. Sci., 14.","DOI":"10.3390\/app14083178"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/JALSI\/2017\/33746","article-title":"Chemical composition of Myrtus communis L. (Myrtaceae) fruits","volume":"12","author":"Qader","year":"2017","journal-title":"J. Appl. Life Sci. Int."},{"key":"ref_9","first-page":"127","article-title":"Evaluation of the effects of three plant species (Myrtus Communis L., Camellia Sinensis L., Zataria Multiflora Boiss.) on the healing process of intraoral ulcers in rats","volume":"18","author":"Hashemipour","year":"2017","journal-title":"J. Dent."},{"key":"ref_10","first-page":"9","article-title":"Effect of Myrtus communis L. plant extract as a drinking water supplement on performance, some blood parameters, egg quality and immune response of older laying hens","volume":"26","author":"Gultepe","year":"2020","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_11","first-page":"263","article-title":"Effect of Myrtus communis leaves extract on blood sugar in rabbits","volume":"3","author":"Aljebory","year":"2014","journal-title":"J. Purity Util. React. Environ."},{"key":"ref_12","first-page":"1","article-title":"Antioxidant Properties of Myrtus Extract: In vitro Assays and in vivo Experimental Animal Study","volume":"15","author":"Denk","year":"2024","journal-title":"Turk. Klin. J. Vet. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Uyarlar, C., Rahman, A., Ozcinar, U., Cetingul, \u0130.S., Gultepe, E.E., and Bayram, I. (2024). Effect of Myrtus communis L. Plant Extract as a Milk Supplement on the Performance, Selected Blood Parameters and Immune Response of Holstein Calves. Animals, 14.","DOI":"10.3390\/ani14050725"},{"key":"ref_14","first-page":"131","article-title":"Effects of dietary myrtle oil (Myrtus communis L.) supplementation on growth performance, meat oxidative stability, meat quality and erythrocyte parameters in quails","volume":"163","author":"Biricik","year":"2012","journal-title":"Revue Med. Vet."},{"key":"ref_15","unstructured":"National Research Council (2001). Nutrient Requirements of Dairy Cattle: Seventh Revised Edition, 2001, The National Academies Press."},{"key":"ref_16","unstructured":"AOAC (2006). Official Methods of Analysis, Association of Official Analytical Chemists. [18th ed.]."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.anifeedsci.2007.04.014","article-title":"A review of plant-derived essential oils in ruminant nutrition and production","volume":"145","author":"Benchaar","year":"2008","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/jsfa.4152","article-title":"Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition","volume":"91","author":"Patra","year":"2011","journal-title":"J. Sci. Food Agric."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.3168\/jds.S0022-0302(04)70039-9","article-title":"A meta-analysis examining the relationship among dietary factors, dry matter intake, and milk and milk protein yield in dairy cows","volume":"87","author":"Hristov","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.micres.2013.10.003","article-title":"Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L.","volume":"169","author":"Aleksic","year":"2014","journal-title":"Microbiol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.3168\/jds.2006-644","article-title":"Invited review: Essential oils as modifiers of rumen microbial fermentation","volume":"90","author":"Calsamiglia","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_22","first-page":"63","article-title":"Effects of Myrtle (Myrtus communis) essential oil on growth performance, carcass characteristics, intestinal morphology, immune response and blood parameters in broiler chickens","volume":"8","author":"Salehifar","year":"2017","journal-title":"J. Livest. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"842","DOI":"10.7482\/0003-9438-56-084","article-title":"The effect of Myrtus communis oil extract on growth performance, serum biochemistry and humoral immune responses in broiler chicks fed diet containing aflatoxin B1","volume":"56","author":"Saei","year":"2013","journal-title":"Arch. Anim. Breed."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1515\/jvetres-2017-0063","article-title":"Effect of Intrauterine Infusion of Momordica Charantia L. on Oxidative Stress and Pregnancy Rate in Infertile Cows","volume":"61","author":"Emre","year":"2017","journal-title":"J. Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.toxlet.2014.01.030","article-title":"The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats","volume":"226","author":"Barlas","year":"2014","journal-title":"Toxicol. Lett."},{"key":"ref_26","first-page":"169","article-title":"Myrtus Species Prevents Reproductive Toxicity Induced by Doxorubicin in Male Mice","volume":"8","author":"Abidli","year":"2015","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_27","first-page":"102","article-title":"Effect of dietary Myrtus communis leaf powder on Quantitative and Qualitative Characteristics of Sperm and Antioxidant Function of Semen and Blood in Arabi Ram","volume":"33","author":"Vakili","year":"2020","journal-title":"Vet. Res. Biol. Prod."},{"key":"ref_28","first-page":"2695","article-title":"Review of pharmacological effects of Glycyrrhiza radix and its bioactive compounds","volume":"34","author":"Gao","year":"2009","journal-title":"China J. Chin. Mater. Medica"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1056\/NEJM199902113400607","article-title":"Acute-phase proteins and other systemic responses to inflammation","volume":"340","author":"Gabay","year":"1999","journal-title":"N. Eng. J. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.aninu.2024.01.012","article-title":"Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade","volume":"17","author":"Wang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.2527\/1995.7392820x","article-title":"Impact of changes in organic nutrient metabolism on feeding the transition dairy cow","volume":"73","author":"Grummer","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1124\/jpet.106.102681","article-title":"Alterations in the rat serum proteome during liver injury from acetaminophen exposure","volume":"318","author":"Merrick","year":"2006","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.rvsc.2011.11.008","article-title":"Metabolic stress and inflammatory response in high-yielding, periparturient dairy cows","volume":"93","author":"Trevisi","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cruciani, S., Santaniello, S., Garroni, G., Fadda, A., Balzano, F., Bellu, E., Sarais, G., Fais, G., Mulas, M., and Maioli, M. (2019). Myrtus Polyphenols, from Antioxidants to Anti-Inflammatory Molecules: Exploring a Network Involving Cytochromes P450 and Vitamin D. Molecules, 24.","DOI":"10.3390\/molecules24081515"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1439-0531.2008.01139.x","article-title":"Long chain fatty acids of diet as factors influencing reproduction in cattle","volume":"43","author":"Santos","year":"2008","journal-title":"Reprod. Domest. Anim."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/4\/632\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T15:10:10Z","timestamp":1771254610000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/4\/632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,16]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ani16040632"],"URL":"https:\/\/doi.org\/10.3390\/ani16040632","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,16]]}}}