{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:34:27Z","timestamp":1792154067432,"version":"4.4.0"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Despite the widespread use of cryopreserved dog semen, reliable predictors of individual semen freezability are still not available. This study investigated the relationship between post-ejaculatory blood plasma concentrations of canine prostate-specific esterase (CPSE) and semen quality following cryopreservation. The CPSE levels were quantified using a canine-specific sandwich-type immunoassay, and sperm quality was evaluated in fresh and frozen\u2013thawed samples using motility, kinematic, and morphological sperm parameters. Cryopreservation resulted in a significant decline in sperm motility and morphology, with marked variability in the magnitude of post-thaw changes among individuals. CPSE showed significant correlations with several morpho-functional parameters in both fresh and frozen\u2013thawed sperm (p \u2264 0.05). However, its strongest predictive value for freezability was observed for total motility (p = 0.004), with a minimum threshold of 53 ng\/mL identifying dogs showing a \u226520% post-thaw motility decline (AUC = 0.785, p = 0.010). These findings suggest that post-ejaculatory CPSE levels in blood plasma may reflect sperm cryoresilience and serve as a potential biomarker of canine sperm freezability.<\/jats:p>","DOI":"10.3390\/ani16050755","type":"journal-article","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:22:21Z","timestamp":1772551341000},"page":"755","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Post-Ejaculatory Blood Plasma Canine Prostate-Specific Esterase Concentrations May Predict Total Motility Decline After Sperm Freezing in Dogs"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-4539","authenticated-orcid":false,"given":"Florin Petri\u0219or","family":"Posastiuc","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"},{"name":"Department of Clinical Sciences II, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Blvd. Splaiul Independentei, 050097 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6017-9263","authenticated-orcid":false,"given":"Guillaume","family":"Domain","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5320-1963","authenticated-orcid":false,"given":"Nicolae Tiberiu","family":"Constantin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences II, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Blvd. Splaiul Independentei, 050097 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7604-6783","authenticated-orcid":false,"given":"Lotte","family":"Spanoghe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8532-2142","authenticated-orcid":false,"given":"Joke","family":"Lannoo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5010-6311","authenticated-orcid":false,"given":"Ann","family":"Van Soom","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Domain, G., Kappen, M., Van Mil, A., De Beijer, I., Van Puyvelde, M., Van Leeuwenberg, R., Spanoghe, L., Posastiuc, F., and Van Soom, A. 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