{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:42:51Z","timestamp":1772818971119,"version":"3.50.1"},"reference-count":29,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Transmissible viral proventriculitis (TVP) is an emerging disease in chickens, linked to chicken proventricular necrosis virus (CPNV), a recently identified birnavirus. Here, we provide the first molecular confirmation of TVP in Bangladesh from a coloured meat-type parent stock (PS) flock, while documenting a contemporaneous white layer flock with consistent clinical signs and characteristic gross lesions. Affected birds exhibited growth retardation, diarrhoea, and increased mortality, alongside hallmark gross changes in proventricular enlargement and wall thickening. From the meat-type PS, proventricular samples were collected for histopathology and molecular diagnostics. Histological analysis revealed severe glandular epithelial damage, necrosis, mononuclear infiltration, epithelial hyperplasia, and metaplasia. Using RT-PCR on nucleic acid extracted from FTA card samples, CPNV was detected. In addition, infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV), and avian reovirus (ARV) nucleic acids were also identified. The amplified CPNV VP1 fragment was sequenced, and phylogenetic analysis placed the Bangladeshi strain within clades of previously reported CPNV isolates. This study represents the first molecularly confirmed report of CPNV associated with TVP in Bangladesh, highlighting the need for active surveillance in commercial and breeder poultry flocks to understand the virus\u2019s epidemiology and support the development of control strategies.<\/jats:p>","DOI":"10.3390\/ani16050789","type":"journal-article","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T16:18:45Z","timestamp":1772554725000},"page":"789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Report and Molecular Confirmation of Chicken Proventricular Necrosis Virus Associated with Transmissible Viral Proventriculitis in Bangladesh"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-9307","authenticated-orcid":false,"given":"P\u00e9ter Ferenc","family":"Dobra","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Budapest, Istv\u00e1n Str. 2, H-1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7372-3144","authenticated-orcid":false,"given":"Barbara","family":"Igriczi","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Budapest, Istv\u00e1n Str. 2, H-1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kitti","family":"Sch\u00f6nhardt","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Budapest, Istv\u00e1n Str. 2, H-1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0069-4914","authenticated-orcid":false,"given":"Lilla","family":"D\u00e9nes","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Budapest, Istv\u00e1n Str. 2, H-1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"K\u0151r\u00f6si","sequence":"additional","affiliation":[{"name":"AgriAL Bt., B\u00e9ri Balogh \u00c1d\u00e1m Str. 42, H-2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2558-2735","authenticated-orcid":false,"given":"Rokshana","family":"Parvin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakibul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Peoples Poultry & Hatchery Ltd., 3 Shahid Tajuddin Ahamed Sharoni, Moghbazar, Dhaka 1217, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3447-4469","authenticated-orcid":false,"given":"M\u00edra","family":"M\u00e1ndoki","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Budapest, Istv\u00e1n Str. 2, H-1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1637\/9504-081610-Reg.1","article-title":"Physical and genomic characteristics identify chicken proventricular necrosis virus (R11\/3 virus) as a novel birnavirus","volume":"55","author":"Guy","year":"2011","journal-title":"Avian Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.3382\/ps.0741799","article-title":"Experimental reproduction of proventriculitis using homogenates of proventricular tissue","volume":"74","author":"Bayyari","year":"1995","journal-title":"Poult. 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