{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:01:05Z","timestamp":1788998465167,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31272445"],"award-info":[{"award-number":["31272445"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31360548"],"award-info":[{"award-number":["31360548"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Nature Science foundation of Inner Mongolia","award":["2017ZD04"],"award-info":[{"award-number":["2017ZD04"]}]},{"name":"Major science and technology projects of Inner Mongolia","award":["2020ZD0003"],"award-info":[{"award-number":["2020ZD0003"]}]},{"name":"Key technologies research and development program of Inner Mongolia","award":["2021GG0402"],"award-info":[{"award-number":["2021GG0402"]}]},{"name":"Inner Mongolia Key Laboratory for Molecular Regulation of the Cell","award":["2021PT0002"],"award-info":[{"award-number":["2021PT0002"]}]},{"name":"Open Competition Sci-Tech Breakthrough Project of Inner Mongolia","award":["2022JBGS0023"],"award-info":[{"award-number":["2022JBGS0023"]}]},{"name":"Central Guidance on Local Science and Technology Development Fund of Hubei Province","award":["2025EIA014"],"award-info":[{"award-number":["2025EIA014"]}]},{"DOI":"10.13039\/501100019035","name":"Science and Technology Program of Hubei Province","doi-asserted-by":"publisher","award":["2025BBB015"],"award-info":[{"award-number":["2025BBB015"]}],"id":[{"id":"10.13039\/501100019035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Porcine reproductive and respiratory syndrome (PRRS), which is caused by the porcine reproductive and respiratory syndrome virus (PRRSV), results in substantial economic losses for the global pig farming industry. A critical step in the infection process is the binding of PRRSV to the CD163 receptor on the surface of porcine alveolar macrophages. This study successfully generated CD163\u2212\/\u2212 Landrace pigs using CRISPR\/Cas9 gene editing technology. Following an experimental challenge with two distinct Type II PRRSV strains, the edited pigs exhibited complete resistance to infection. Virological and pathological examinations confirmed the absence of viral replication and the presence of characteristic pulmonary lesions and other organ damage in CD163\u2212\/\u2212 pigs. In contrast, wild-type control pigs exhibited high viral loads and severe pulmonary lesions, as well as damage to other organs. Our findings provide direct evidence that CD163 is an essential receptor for PRRSV infection in vivo. The CD163\u2212\/\u2212 pig model offers an effective genetic strategy for breeding pigs with an inherent resistance to PRRSV.<\/jats:p>","DOI":"10.3390\/ani16050850","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T15:49:42Z","timestamp":1773071382000},"page":"850","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pigs with CD163 Mutation Conferred PRRSV Resistance"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0423-611X","authenticated-orcid":false,"given":"Changbao","family":"Wu","sequence":"first","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heyao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Life Science, Inner Mongolia University, Hohhot 010000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Institute of Animal Sciences and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"ref_1","first-page":"93","article-title":"Recurrent reproductive failure due to severe placental villitis of unknown etiology","volume":"24","author":"Russell","year":"1980","journal-title":"J. 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