{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T11:21:31Z","timestamp":1773746491801,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005632","name":"National Centre for Research and Development","doi-asserted-by":"publisher","award":["PBS3\/A8\/32\/2015"],"award-info":[{"award-number":["PBS3\/A8\/32\/2015"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The study aimed to determine the effect of newly developed multi-strain synbiotics on the hygienic quality of feed for pigs and pork edible raw materials originating from animals supplemented with synbiotics. The trial was conducted on 54 feed samples and 54 pigs. Animals were divided into six groups corresponding to three synbiotic preparations (A, B, C), two positive controls with probiotics (D, E), and a negative control (K) with no feed additive. The supplementation of the sow\u2019s basal diet with the feed additive started 10 days before farrowing and continued for 38 days, covering the lactation period. The diet of piglets from groups A-E was supplemented with the feed additive starting from two weeks of age until slaughter at 24 weeks of age. Feed and animal raw materials were tested for the presence of Salmonella spp., Campylobacter spp., L. monocytogenes, Clostridium spp., C. perfringens, C. botulinum, and the count of AMB, TPC, fungi, ASFB, C. perfringens, Enterobacteriaceae family, E. coli, presumptive B. cereus, CoPS, HS, LAB, yeast probiotic strains, and Enterococcus spp. Statistically significant differences were found between individual groups in the count of C. perfringens, AMB, TPC, Enterococcus spp., and LAB in all feeds tested. Moreover, synbiotics A, B, and C lowered the count of AMB, TPC, and LAB, and synbiotics A and C decreased Enterobacteriaceae family contamination in both total raw materials as well as raw materials of fatteners. The higher effectiveness of synbiotics was associated with a higher number of probiotic strains in one preparation. Our study found that dietary supplementation of synbiotics alters the occurrence of C. perfringens in feed and enhances the hygienic quality of edible pork raw materials.<\/jats:p>","DOI":"10.3390\/ani16060933","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T10:21:41Z","timestamp":1773742901000},"page":"933","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Synbiotics on Hygienic Quality of Feed and Pork"],"prefix":"10.3390","volume":"16","author":[{"given":"El\u017cbieta","family":"Kukier","sequence":"first","affiliation":[{"name":"School of Medical & Health Sciences, VIZJA University, 59 Okopowa Street, 01-043 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8204-5856","authenticated-orcid":false,"given":"\u0141ukasz","family":"Bocian","sequence":"additional","affiliation":[{"name":"Department of Research Support, National Veterinary Research Institute, 57 Partyzant\u00f3w Avenue, 24-100 Pulawy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5998-5156","authenticated-orcid":false,"given":"Monika","family":"Pytka","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3161-1707","authenticated-orcid":false,"given":"Katarzyna","family":"\u015ali\u017cewska","sequence":"additional","affiliation":[{"name":"Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171\/173 W\u00f3lcza\u0144ska Street, 90-924 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"ref_1","first-page":"116","article-title":"Anti- und probiotica","volume":"25","author":"Vergin","year":"1954","journal-title":"Hipokrates"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/s41575-020-0344-2","article-title":"The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics","volume":"17","author":"Swanson","year":"2020","journal-title":"Nat. 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