{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:27:46Z","timestamp":1785414466805,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["NRCT5-RGJ63004-080"],"award-info":[{"award-number":["NRCT5-RGJ63004-080"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"award":["NRCT5-RGJ63004-080"],"award-info":[{"award-number":["NRCT5-RGJ63004-080"]}],"id":[{"id":"https:\/\/ror.org\/018wfhg78","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Although internal fixation and surgical approaches promote fracture healing, some outcomes remain unsatisfactory. Advanced platelet-rich fibrin (A-PRF) has been shown to provide more growth factors, and in vitro cell proliferation has not been evaluated for treating bone fractures in veterinary medicine. The purpose of this study was to evaluate the bone-healing activity of A-PRF in traumatic canine fractures. Twelve dogs with single radius\u2013ulna or tibia\u2013fibula fractures were randomly assigned to two groups: a control group and an A-PRF group. Both groups were treated with a locking compression plate and screws and received pain control. Post-operatively, dogs were evaluated for serum C-reactive protein (CRP) levels and post-operative pain scores on days 1, 3, and 7. Lameness and weight-bearing scores were evaluated on days 1, 3, 7, 14, 30, and 60. Bone healing was assessed at 2 weeks, 1 month, and 2 months using calculated relative bone density (%). Compared with the control, the A-PRF group showed higher bone density at 2 months and lower lameness at 14 days post-operatively. Although the CRP level, an inflammation response marker, was higher in the A-PRF group within one day. No significant difference in pain score was observed. In conclusion, A-PRF serves as an effective adjunctive therapy for promoting bone healing when treating canine appendicular fractures with surgical internal fixation.<\/jats:p>","DOI":"10.3390\/ani16081276","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:10:44Z","timestamp":1776787844000},"page":"1276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Advanced Platelet-Rich Fibrin on Bone Healing in the Treatment of Canine Appendicular Fractures"],"prefix":"10.3390","volume":"16","author":[{"given":"Ravisa","family":"Warin","sequence":"first","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeyanat","family":"Vongchan","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2327-195X","authenticated-orcid":false,"given":"Witaya","family":"Suriyasathaporn","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Asian Satellite Campuses Institute-Oversea Campus, Nagoya University, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5786-2769","authenticated-orcid":false,"given":"Ratchadaporn","family":"Boripun","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6621-0057","authenticated-orcid":false,"given":"Kanawee","family":"Warrit","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7710-4092","authenticated-orcid":false,"given":"Luddawon","family":"Somrup","sequence":"additional","affiliation":[{"name":"Northern Regional Veterinary Healthcare and Learning Center, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1271-5120","authenticated-orcid":false,"given":"Kittidaj","family":"Tanongpitchayes","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Prince of Songkhla University, SongKhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimnipa","family":"Jieraviriyapun","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7278-6036","authenticated-orcid":false,"given":"Wanna","family":"Suriyasathaporn","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1055\/s-0038-1633097","article-title":"Incidence of canine appendicular musculoskeletal disorders in 16 veterinary teaching hospitals from 1980 through 1989","volume":"7","author":"Johnson","year":"1994","journal-title":"Vet. 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