{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:57:05Z","timestamp":1777442225296,"version":"3.51.4"},"reference-count":94,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study examined the effects of in ovo silver nanoparticle (AgNP) administration on broiler chicken hatchability, growth performance, physiological and metabolic status, antioxidant capacity, and immune responses. A total of 300 fertilized Cobb500 eggs were assigned to five treatments (5 replicates\/treatment, 12 eggs\/replicate): non-injected control; vehicle control (0.2 mL sterile 0.9% NaCl\/egg); and AgNPs at 10, 15, or 20 ppm injected into their amniotic sac on day (d) 18 of incubation. After hatching, 48 chicks\/treatment were assigned to 6 replicate cages (8 birds\/replicate) for 35 d. In ovo AgNP administration improved hatchability and reduced embryonic mortality (p &lt; 0.05). Post-hatch, birds originating from AgNP-injected eggs had an improved body weight, body weight gain, and feed conversion ratio, with lower feed intake (p &lt; 0.05). AgNP administration elevated hemoglobin, packed cell volume, growth hormone, serum proteins, alanine aminotransferase activity, and creatinine levels (p &lt; 0.05). AgNPs also improved the lipid profile and increased total antioxidant capacity, catalase and superoxide dismutase activities, lysozyme activity, complement 3, and cytokine concentrations and reduced malondialdehyde levels (p &lt; 0.05). Effects were dose-dependent, with 15 ppm generally leading to better outcomes. These findings suggest that 15 ppm AgNP in ovo administration enhances embryonic viability, physiological resilience, and post-hatch performance in broiler chickens.<\/jats:p>","DOI":"10.3390\/ani16091349","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:33:37Z","timestamp":1777376017000},"page":"1349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Ovo Administration of Silver Nanoparticles Enhances Post-Hatch Growth Performance, Metabolic and Physiological Responses, and Immune Function in Broiler Chickens"],"prefix":"10.3390","volume":"16","author":[{"given":"Hanan","family":"Al-Khalaifah","sequence":"first","affiliation":[{"name":"Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research (KISR), Kuwait City 13109, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8902-8311","authenticated-orcid":false,"given":"Samar A.","family":"Tolba","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig P.O. Box 44511, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8196-0713","authenticated-orcid":false,"given":"Inas I.","family":"Ismail","sequence":"additional","affiliation":[{"name":"Poultry Breeding Department, Animal Production Research Institute, Agriculture Research Center, Giza P.O. Box 12618, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azza A.","family":"Megahid","sequence":"additional","affiliation":[{"name":"Poultry Breeding Department, Animal Production Research Institute, Agriculture Research Center, Giza P.O. Box 12618, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman S.","family":"Osman","sequence":"additional","affiliation":[{"name":"Poultry Breeding Department, Animal Production Research Institute, Agriculture Research Center, Giza P.O. Box 12618, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed H.","family":"Rabie","sequence":"additional","affiliation":[{"name":"Poultry Breeding Department, Animal Production Research Institute, Agriculture Research Center, Giza P.O. Box 12618, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2577-2993","authenticated-orcid":false,"given":"Ahmed","family":"Gouda","sequence":"additional","affiliation":[{"name":"Department of Animal Production, National Research Center, El Buhouth St., Dokki, Giza P.O. Box 12622, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tona, K., Voemesse, K., N\u2019nAnl\u00e9, O., Oke, O.E., Kouame, Y.A.E., Bilalissi, A., Meteyake, H., and Oso, O.M. (2022). 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