{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T08:05:14Z","timestamp":1784880314222,"version":"3.55.0"},"reference-count":67,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Provincial Modern Agriculture (Aquaculture) Industry Technology System Project","award":["HARS-07"],"award-info":[{"award-number":["HARS-07"]}]},{"name":"Hunan Agricultural Science and Technology Innovation Fund","award":["2025CX82"],"award-info":[{"award-number":["2025CX82"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Ochetobibus elongatus (Kner) is a migratory fish found in the Yangtze River basin and areas south of it, and listed as a critically endangered (CR) fish on the China Red List of Vertebrates. To achieve group recovery and artificial breeding, this study investigated the dietary characteristics of O. elongatus based on high-throughput sequencing of its intestinal contents, and its digestive system morphology, and its histology. Results showed that the digestive system of O. elongatus lacked a stomach and mainly consisted of the oropharynx, pharyngeal teeth, esophagus, intestine, and anus. The gut index was 0.88, with clear segmentation of the foregut, midgut, and hindgut, and the visceral mass index was 7.35%. Histological analysis of the digestive system revealed the presence of keratinized dental plates or pharyngeal teeth in the pharynx, as well as a high density of taste bud cells in the soft palate of the oral cavity. The surface layer of the intestinal villi contained numerous mucous cells, with the average number of mucous cells per villus gradually increasing from the esophagus to the hindgut, and the foregut having the longest and most abundant mucosal folds. The esophagus exhibited well-developed circular and longitudinal muscle layers, while in the hindgut, both the circular and longitudinal muscle layers were slightly thicker than those in the midgut. High-throughput sequencing of the intestinal contents of O. elongatus revealed the following phyla based on 18S V4 meta-barcoding: Chlorophyta, Diatoms, Arthropoda, Basidiomycetes, and Ascomycetes, with the genus Hypophthalmichthys and algae being the main classifications. In contrast, based on COI meta-barcoding, the study newly identified the phyla Cnidaria and Mollusca, with the genera Chlorophyta, Scenedesmus, Pectinodesmus, and zooplankton such as Pseudodiaptomus. Metagenomic sequencing revealed that the gut microbiota at the phylum level was predominantly composed of Pseudomonadota, Ascomycota, Basidiomycota, Chytridiomycota, and Bacillota, with key genera including Cetobacter, Pseudomonas, Acinetobacter, Aeromonas, and Clostridium. This study indicates that O. elongatus is an omnivore with carnivorous tendencies. Basic biological research on O. elongatus is of great significance for the restoration of the population, artificial breeding, and the development of its artificially formulated feed. It also provides important data for the formulation of biodiversity conservation measures.<\/jats:p>","DOI":"10.3390\/ani16091369","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T12:31:56Z","timestamp":1777465916000},"page":"1369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Ochetoibus elongatus (Kner) Dietary Habits Based on Digestive System Morphology, Histology, and Intestinal Content Sequencing Technology"],"prefix":"10.3390","volume":"16","author":[{"given":"Feng","family":"Gao","sequence":"first","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"},{"name":"Yuelushan Laboratory, Hunan Academy of Agricultural Sciences, Changsha 410153, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1293-9495","authenticated-orcid":false,"given":"Zhiliang","family":"Zuo","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qifan","family":"Wu","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hewei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Peng","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zou","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"},{"name":"Yuelushan Laboratory, Hunan Academy of Agricultural Sciences, Changsha 410153, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guomin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Tian","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Feng","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Xie","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"},{"name":"Yuelushan Laboratory, Hunan Academy of Agricultural Sciences, Changsha 410153, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9228-9179","authenticated-orcid":false,"given":"Lu","family":"Tian","sequence":"additional","affiliation":[{"name":"Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410005, China"},{"name":"Yuelushan Laboratory, Hunan Academy of Agricultural Sciences, Changsha 410153, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","first-page":"76","article-title":"Nitrogen and phosphorus excretion of fishes with different feeding habits and their role in nutrient cycling in Xiaojiang River","volume":"45","author":"Tang","year":"2024","journal-title":"J. Hydroecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1111\/jfb.12489","article-title":"Feeding habits and ontogenic changes in digestive enzyme patterns in five freshwater teleosts","volume":"85","author":"Solovyev","year":"2015","journal-title":"J. Fish Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1034\/j.1600-0633.2003.00010.x","article-title":"Atlantic salmon Salmo salar L. brown trout Salmo trutta L. and Arctic charr Salvelinus alpinus (L.): A review of aspects of their life histories","volume":"12","author":"Klemetsen","year":"2010","journal-title":"Ecol. Freshw. Fish"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1007\/s10641-018-0794-3","article-title":"Feeding ecology of the k\u0131z\u0131l\u0131rmak toothcarp, aphanius marassantensis: Ontogenetic shift and seasonal diet variation","volume":"101","author":"Kirankaya","year":"2018","journal-title":"Environ. Biol. Fishes"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/ajfar\/2023\/v23i4605","article-title":"Exploring fish eating habits: Factors influencing feeding behavior in tropical fish","volume":"23","author":"Pratiwy","year":"2023","journal-title":"Asian J. Fish. Aquat. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1111\/jam.12663","article-title":"Comparative study on gastrointestinal microbiota of eight fish species with different feeding habits","volume":"117","author":"Li","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tesso, T.A., and Nurgi, L.K. (2025). Feeding habit and diet composition of three fish species inhabiting Sor River, Baro-Akobo Basin of Ethiopia, East Africa. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0325035"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"500","DOI":"10.17520\/biods.2016076","article-title":"Red List of China\u2019s vertebrates","volume":"24","author":"Jiang","year":"2016","journal-title":"Biodivers. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/s41597-024-04223-x","article-title":"A chromosome-level genome assembly of critically endangered Ochetobius elongatus","volume":"11","author":"Li","year":"2024","journal-title":"Sci. Data"},{"key":"ref_10","first-page":"19","article-title":"Genetic structure analysis of Ochetobius elongatus between Yangtze River and Pearl River using multiple loci","volume":"18","author":"Chen","year":"2022","journal-title":"South China Fish. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Cai, F.T., Qi, Z.Y., Hu, Z.H., Zhai, D.D., Chen, Y.Y., Xiong, F., and Liu, H.Y. (2025). Revealing phenotypic differentiation in Ochetobius elongatus from the middle Yangtze River through geometric morphometrics. Animals, 15.","DOI":"10.3390\/ani15192870"},{"key":"ref_12","first-page":"28","article-title":"Successful artificial reproduction of Ochetobius elongatus","volume":"2","author":"Ge","year":"1979","journal-title":"Freshw. Fish."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s12686-018-1075-3","article-title":"Development and characterization of 26 SNP markers in Ochetobius elongatus based on restriction site-associated DNA sequencing (RAD-seq)","volume":"12","author":"Yang","year":"2020","journal-title":"Conserv. Genet. Resour."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.17520\/biods.2018121","article-title":"Genetic diversity and group demographic history of Ochetobius elongatus in the middle and lower reaches of the Xijiang River","volume":"26","author":"Yang","year":"2018","journal-title":"Biodivers. Sci."},{"key":"ref_15","first-page":"1736","article-title":"Genetic diversity and group historical dynamics of Ochetobius elongatus in the middle reaches of the Yangtze River","volume":"48","author":"Zhai","year":"2024","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_16","first-page":"361","article-title":"Studies on digestive system and different feeding habits of some fishes in freshwater","volume":"20","author":"Zeng","year":"1998","journal-title":"J. Southwest Agric. Univ."},{"key":"ref_17","first-page":"63","article-title":"Histological observation of digestive system in blue mudhopper Scartelaos vividis","volume":"32","author":"Cao","year":"2013","journal-title":"Fish. Sci."},{"key":"ref_18","first-page":"101","article-title":"Differences of gastrointestinal index and digestive enzyme activity in different ages of Culter alburnus in Khanka Lake","volume":"44","author":"Yin","year":"2013","journal-title":"J. Northeast Agric. Univ."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1007\/s11160-024-09853-3","article-title":"Diet and habitat as determinants of intestine length in fishes","volume":"34","author":"Clements","year":"2024","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8591249","DOI":"10.1155\/2017\/8591249","article-title":"Histomorphological description of the digestive system of pebbly fish, Alestes baremoze (Joannis, 1835)","volume":"2017","author":"Kasozi","year":"2017","journal-title":"Sci. World J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"13218","DOI":"10.1002\/ece3.8045","article-title":"Phylogeny, body morphology, and trophic level shape intestinal traits in coral reef fishes","volume":"11","author":"Ghilardi","year":"2021","journal-title":"Ecol. Evol."},{"key":"ref_22","first-page":"379","article-title":"The morphological characteristics of the digestive system in two coexisting balitoridaes","volume":"43","author":"Hu","year":"2019","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1111\/jfb.14821","article-title":"Morphology of the digestive system in carnivorous freshwater dourado Salminus brasiliensis","volume":"99","author":"Alves","year":"2021","journal-title":"J. Fish Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e10499","DOI":"10.1002\/ece3.10499","article-title":"A comparison of digestive strategies for fishes with different feeding habits: Digestive enzyme activities, intestinal morphology, and gut microbiota","volume":"13","author":"Jiao","year":"2023","journal-title":"Ecol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/azo.12156","article-title":"Morphology of the digestive tract of the whitemouth croaker Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)","volume":"98","author":"Andrade","year":"2017","journal-title":"Acta Zool."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Weinrauch, A.M., Schaefer, C.M., and Goss, G.G. (2019). Activity and post-prandial regulation of digestive enzyme activity along the Pacific hagfish (Eptatretus stoutii) alimentary canal. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0215027"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e11375","DOI":"10.1002\/ece3.11375","article-title":"Linking anatomical and histological traits of the digestive tract to resource consumption and assimilation of omnivorous tetra fishes","volume":"14","author":"Neves","year":"2024","journal-title":"Ecol. Evol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/jfb.16002","article-title":"Comparative study of gut content microbiota in freshwater fish with different feeding habits: A case study of an urban lake","volume":"106","author":"Li","year":"2025","journal-title":"J. Fish Biol."},{"key":"ref_29","first-page":"22","article-title":"Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea","volume":"18","author":"Chen","year":"2022","journal-title":"South China Fish. Sci."},{"key":"ref_30","first-page":"532","article-title":"Dietary analysis of ferocious fishes in Lake Poyang based on stomach content analy and eDNA Metabarcoding technology","volume":"37","author":"Zhao","year":"2025","journal-title":"J. Lake Sci."},{"key":"ref_31","first-page":"393","article-title":"Preliminary dietary analysis of Hyporhamphus sajori juveniles based on DNA metabarcoding","volume":"30","author":"Li","year":"2023","journal-title":"J. Fish. Sci. China"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.aaf.2024.01.002","article-title":"The difference of the composition and digestive enzymes of gut microbiome in herbivorous blunt snout bream (Megalobrama amblycephala) and carnivorous largemouth bass (Micropterus salmoides)","volume":"10","author":"Yu","year":"2025","journal-title":"Aquac. Fish."},{"key":"ref_33","first-page":"75","article-title":"Comparative analysis of the composition and diversity of the intestinal microflora of four fishes with different feeding habits","volume":"45","author":"Rong","year":"2024","journal-title":"Prog. Fish. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pan, W.N., Qin, C.X., Zuo, T., Yu, G., Zhu, W.T., Ma, H.M., and Xi, S.G. (2021). Is metagenomic analysis an effective way to analyze fish feeding habits? A case of the yellowfin sea bream Acanthopagrus latus (Houttuyn) in daya bay. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.634651"},{"key":"ref_35","unstructured":"Ozaki, K. (1983). Fish Digestive Physiology, Shanghai Scientific and Technical Press."},{"key":"ref_36","first-page":"32","article-title":"Effects of water temperature on distribution and type of skin mucus cells and mucus immune factors of mandarinfish Siniperca chuatsi","volume":"43","author":"Shi","year":"2024","journal-title":"Fish. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kennedy, N.A., Walker, A.W., Berry, S.H., Duncan, S.H., Farquarson, F.M., Louis, P., Thomson, J.M., Satsangi, J., Flint, H.J., and Parkhill, J. (2014). The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088982"},{"key":"ref_38","unstructured":"Xie, C.X., Ma, X.F., and Qin, J.H. (2016). Studies on Biodiversity and Conservation Technology of Glyptosternum maculatum in the Yarlung Zangbo River, Science Press."},{"key":"ref_39","first-page":"410","article-title":"Histological study on the digestive tract of cultivated Gobiocypris rarus","volume":"32","author":"Wang","year":"2013","journal-title":"Sichuan J. Zool."},{"key":"ref_40","first-page":"45","article-title":"Morphological and histological observation of digestive system in slender top-mouth gudgeon Pseudorasbora elongate","volume":"34","author":"Yang","year":"2021","journal-title":"Chin. J. Fish."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1890\/08-1150.1","article-title":"Predator size, prey size, and dietary niche breadth relationships in marine predators","volume":"90","author":"Costa","year":"2009","journal-title":"Ecology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1093\/icb\/icv038","article-title":"Bottom feeding and beyond: How the premaxillary protrusion of cypriniforms allowed for a novel kind of suction feeding","volume":"55","author":"Hernandez","year":"2015","journal-title":"Integr. Comp. Biol."},{"key":"ref_43","first-page":"32","article-title":"Study on digestive tube index and activity distribution of digestive enzymes in Macculochella peeli","volume":"14","author":"An","year":"2017","journal-title":"J. Yangtze Univ."},{"key":"ref_44","first-page":"80","article-title":"Histology and distribution of mucous cells in digestive system of Bahaba flavolabiata","volume":"40","author":"Zhao","year":"2019","journal-title":"Prog. Fish. Sci."},{"key":"ref_45","first-page":"120","article-title":"Anatomy and histological observation of digestive system in Schizothorax eurystomus","volume":"16","author":"Wei","year":"2020","journal-title":"South China Fish. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.3724\/SP.J.1035.2012.01164","article-title":"Ontogeny of the digestive tracts in grass carp (Ctenopharyngodon idellus), yellowcheck carp (Elopichthys bambusa) and topmouth culter (Culter alburnus)","volume":"36","author":"Ruan","year":"2012","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Moraes, G., and de Almeida, L.C. (2020). Nutrition and functional aspects of digestion in fish. Biology and Physiology of Freshwater Neotropical Fish, Academic Press.","DOI":"10.1016\/B978-0-12-815872-2.00011-7"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zhu, K.F., Jiang, M.Y., Yan, M.Y., Huang, Y., Yang, T.L., and Zhu, C.H. (2025). Characteristics and functions of different intestinal segments in juvenile greater amberjack (Seriola dumerili). Animals, 15.","DOI":"10.3390\/ani15111672"},{"key":"ref_49","first-page":"101278","article-title":"Functional insights of digestion, absorption, and immunity in different segments of the intestine in Hemibarbus labeo from transcriptomic analysis","volume":"52","author":"Lv","year":"2024","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_50","first-page":"75","article-title":"Changes in the number of intestinal mucous cells of juvenile southern catfish Silurus meridionalis following feeding, starvation, and refeeding","volume":"20","author":"Li","year":"2013","journal-title":"Chin. J. Fish. Sci."},{"key":"ref_51","first-page":"28","article-title":"Histological and histochemical study of digestive system of Rhabdosargus sarba","volume":"4","author":"Gou","year":"2008","journal-title":"South China Fish. Sci."},{"key":"ref_52","first-page":"150","article-title":"Structure and ultrastructure of alimentary canal of Cynoglossus semilaevis","volume":"38","author":"Li","year":"2017","journal-title":"Prog. Fish. Sci."},{"key":"ref_53","first-page":"1685","article-title":"Effects on mucous cells distribution in digestive tract of Scortum barcoo","volume":"40","author":"Xie","year":"2018","journal-title":"Chin. J. Cell Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1111\/are.14592","article-title":"Study on the adaptability of common carp (Cyprinus carpio) to diets from the perspective of digestion and absorption","volume":"51","author":"Zhao","year":"2020","journal-title":"Aquac. Res."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Bosi, G., Lorenzoni, M., Carosi, A., and Sayyaf, D.B. (2020). Mucosal hallmarks in the alimentary canal of northern pike Esox lucius (Linnaeus). Animals, 10.","DOI":"10.3390\/ani10091479"},{"key":"ref_56","first-page":"394","article-title":"Analysis of feeding habits of Mystus guttatus based on DNA macrobarcode technology","volume":"44","author":"Meng","year":"2025","journal-title":"J. Fish Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Liang, L., Li, J.J., Fang, S.Y., Jiang, C., Bi, S., and Zhou, L. (2026). From sequences to food webs: DNA metabarcoding reshapes fish trophic ecology. Animals, 16.","DOI":"10.3390\/ani16030443"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Bereded, N.K., Abebe, G.B., Fanta, S.W., Curto, M., Waidbacher, H., Meimberg, H., and Domig, K.J. (2021). The impact of sampling season and catching site (wild and aquaculture) on gut microbiota composition and diversity of Nile tilapia (Oreochromis niloticus). Biology, 10.","DOI":"10.3390\/biology10030180"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Ruzauskas, M., Armalyt\u0117, J., Lastauskien\u0117, E., \u0160iug\u017edinien\u0117, R., Klimien\u0117, I., Mockeli\u016bnas, R., and Bartkien\u0117, E. (2021). Microbial and antimicrobial resistance profiles of microbiota in common carps (Cyprinus carpio) from aquacultured and wild fish groups. Animals, 11.","DOI":"10.3390\/ani11040929"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Romero, J., D\u00edaz, O., Miranda, C.D., and Rojas, R. (2022). Red cusk-eel (Genypterus chilensis) gut microbiota description of wild and aquaculture specimens. Microorganisms, 10.","DOI":"10.3390\/microorganisms10010105"},{"key":"ref_61","first-page":"1975","article-title":"Evaluation of nutritional and flavor quality of wild and cultured Coreius heterodon (Bleeker)","volume":"56","author":"He","year":"2025","journal-title":"J. Southwest Agric."},{"key":"ref_62","first-page":"281","article-title":"Analysis and evaluation of muscle nutritional composition among wild, domesticated and cultured Gymnocypris potanini firmispinatus","volume":"40","author":"Zhang","year":"2025","journal-title":"J. Dalian Ocean Univ."},{"key":"ref_63","first-page":"51","article-title":"Comparison of nutrients and volatile substances in muscles between cultured and wild mullet Liza haematocheila","volume":"43","author":"Li","year":"2024","journal-title":"J. Fish Sci."},{"key":"ref_64","first-page":"73","article-title":"Analysis on intestine microbial community structure of three carnivorous fishes in Qianxia lake, China","volume":"35","author":"Xiao","year":"2022","journal-title":"Chin. J. Fish."},{"key":"ref_65","first-page":"963","article-title":"Intestinal microbial community of wild and cultured Onychostoma macrolepi based on 16S rRNA high-throughput sequencing","volume":"30","author":"Gou","year":"2021","journal-title":"Acta Agric. Boreali-Occident. Sin."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.fsi.2021.12.008","article-title":"Stress responses of the intestinal digestion, antioxidant status, microbiota and non-specific immunity in Songpu mirror carp (Cyprinus carpio L.) under starvation","volume":"120","author":"Zhao","year":"2022","journal-title":"Fish Shellfish Immun."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Liang, Y.Y., Wang, Z.J., Gao, N., Qi, X.X., Zeng, J.T., Cui, K., Lu, W.X., and Bai, S.J.V. (2024). Variations and interseasonal changes in the gut microbial communities of seven wild fish species in a natural lake with limited water exchange during the closed fishing season. Microorganisms, 12.","DOI":"10.3390\/microorganisms12040800"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/9\/1369\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T07:52:32Z","timestamp":1784879552000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/9\/1369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":67,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["ani16091369"],"URL":"https:\/\/doi.org\/10.3390\/ani16091369","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]}}}