{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:28:11Z","timestamp":1785421691084,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002842","name":"Chiang Mai University","doi-asserted-by":"publisher","award":["Fundamental Fund 2026"],"award-info":[{"award-number":["Fundamental Fund 2026"]}],"id":[{"id":"10.13039\/501100002842","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Fundamental Fund 2026"],"award-info":[{"award-number":["Fundamental Fund 2026"]}],"id":[{"id":"https:\/\/ror.org\/05m2fqn25","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["R68IN00678"],"award-info":[{"award-number":["R68IN00678"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"award":["R68IN00678"],"award-info":[{"award-number":["R68IN00678"]}],"id":[{"id":"https:\/\/ror.org\/000025p04","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Melanoma-associated antigen (MAGE) is a promising immunotherapeutic target for cancer vaccines. Heat shock protein 110 (HSP110), expressed in various tumors, including canine mammary tumors, serves as a molecular marker. This study aimed to develop a recombinant fusion protein by linking HSP110 with MAGE-B10 to target MAGE-B10-expressed tumors and assess immune response efficacy. The recombinant MAGE-B10-HSP110 (rMAGE-B10-HSP110) fusion protein was constructed, and separate recombinant MAGE-B10 (rMAGE-B10) and recombinant HSP110 (rHSP110) proteins were also prepared for comparison. Our study on mice is distributed across five treatment groups: the rMAGE-B10-HSP110 fusion protein, rMAGE-B10, rHSP110, a protein mixture, and a PBS control. Antibody responses specific to canine MAGE-B10 were measured using ELISA, while splenocyte activation, proliferation, and cytokine production were analyzed using flow cytometry. The results showed significantly higher antibody responses in mice immunized with the rMAGE-B10-HSP110 fusion protein compared to those receiving PBS or rHSP110 on days 7, 14, and 21. The proportion of CD3+ and CD4+ lymphocytes were significantly higher in these mice (p &lt; 0.05). rMAGE-B10-HSP110 fusion protein immunization also resulted in increased CD69+ lymphocytes and higher IFN-\u03b3 levels in stimulated lymphocytes (p &lt; 0.05). In conclusion, the rMAGE-B10-HSP110 fusion protein effectively stimulates both innate and adaptive immune responses. Further in vivo investigation is recommended.<\/jats:p>","DOI":"10.3390\/ani16091374","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:01:18Z","timestamp":1777474878000},"page":"1374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Recombinant MAGE-B10-HSP110 Fusion Protein Enhances Innate and Adaptive Immune Responses in Mice: A Potential Vaccine Candidate for Canine Mammary Tumors"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0126-0758","authenticated-orcid":false,"given":"Anucha","family":"Muenthaisong","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8747-2912","authenticated-orcid":false,"given":"Kanokwan","family":"Sangkakam","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5311-2220","authenticated-orcid":false,"given":"Pongpisid","family":"Koonyosying","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Faculty of Medicine, Nation University, Chiang Mai 50210, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanya","family":"Varinrak","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"},{"name":"Veterinary Diagnostic and Technology Transfer Center, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2329-8802","authenticated-orcid":false,"given":"Nattawooti","family":"Sthitmatee","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0195-5616(03)00020-2","article-title":"Canine mammary gland tumors","volume":"33","author":"Sorenmo","year":"2003","journal-title":"Vet. Clin. Small Anim. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Salas, Y., M\u00e1rquez, A., Diaz, D., and Romero, L. (2015). Epidemiological study of mammary tumors in female dogs diagnosed during the period 2002\u20132012: A growing animal health problem. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0127381"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1002\/1097-0142(197008)26:2<419::AID-CNCR2820260225>3.0.CO;2-U","article-title":"Comparison of age, sex, and incidence rates in human and canine breast cancer","volume":"26","author":"Schneider","year":"1970","journal-title":"Cancer"},{"key":"ref_4","first-page":"182","article-title":"Spontaneous canine mammary tumors. A model for monoclonal antibody diagnosis and treatment of human breast cancer","volume":"71","author":"Mottolese","year":"1994","journal-title":"Lab. Investig."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Valdivia, G., Alonso-Diez, \u00c1., P\u00e9rez-Alenza, D., and Pe\u00f1a, L. (2021). From conventional to precision therapy in canine mammary cancer: A comprehensive review. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.623800"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1532-950X.2007.00351.x","article-title":"Mammary tumor recurrence in bitches after regional mastectomy","volume":"37","author":"Stratmann","year":"2008","journal-title":"Vet. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1046\/j.1439-0442.2001.00336.x","article-title":"Adjuvant post-operative chemotherapy in bitches with mammary cancer","volume":"48","author":"Karayannopoulou","year":"2001","journal-title":"J. Vet. Med. A"},{"key":"ref_8","first-page":"1184","article-title":"Postoperative adjuvant treatment of invasive malignant mammary gland tumors in dogs with doxorubicin and docetaxel","volume":"20","author":"Simon","year":"2006","journal-title":"J. Vet. Intern. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1476-5829.2007.00143.x","article-title":"Adjuvant gemcitabine after surgical removal of aggressive malignant mammary tumours in dogs","volume":"6","author":"Marconato","year":"2008","journal-title":"Vet. Comp. Oncol."},{"key":"ref_10","first-page":"375","article-title":"Canine malignant mammary gland neoplasms with advanced clinical staging treated with carboplatin and cyclooxygenase inhibitors","volume":"26","author":"Lavalle","year":"2012","journal-title":"In Vivo"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5047","DOI":"10.1158\/1078-0432.CCR-15-0685","article-title":"How do cytotoxic lymphocytes kill cancer cells?","volume":"21","author":"Anel","year":"2015","journal-title":"Clin. Cancer Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1126\/science.1840703","article-title":"A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma","volume":"254","author":"Traversari","year":"1991","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1002\/(SICI)1521-4141(199910)29:10<3329::AID-IMMU3329>3.0.CO;2-7","article-title":"A MAGE-A4 peptide presented by HLA-A2 is recognized by cytolytic T lymphocytes","volume":"29","author":"Duffour","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"ref_14","first-page":"4072","article-title":"A MAGE-A1 HLA-A*0201 epitope identified by mass spectrometry","volume":"61","author":"Pascolo","year":"2001","journal-title":"Cancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/s00262-005-0053-2","article-title":"A new MAGE-4 antigenic peptide recognized by cytolytic T lymphocytes on HLA\u2013A24 carcinoma cells","volume":"55","author":"Ottaviani","year":"2006","journal-title":"Cancer Immunol. Immunother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"685","DOI":"10.4049\/jimmunol.1001775","article-title":"A TCR targeting the HLA-A*0201\u2013restricted epitope of MAGE-A3 recognizes multiple epitopes of the MAGE-A antigen superfamily in several types of cancer","volume":"186","author":"Chinnasamy","year":"2011","journal-title":"J. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2739\/kurumemedj.44.43","article-title":"Detection of MAGE-1 tumor antigen in brain tumor","volume":"44","author":"Kuramoto","year":"1997","journal-title":"Kurume Med. J."},{"key":"ref_18","first-page":"3971","article-title":"Combined use of oligonucleotide and tissue microarrays identifies cancer\/testis antigens as biomarkers in lung carcinoma","volume":"62","author":"Sugita","year":"2002","journal-title":"Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chen, Y.T., Ross, D.S., Chiu, R., Zhou, X.K., Chen, Y.Y., Lee, P., Hoda, S.A., Simpson, A.J., Old, L.J., and Caballero, O. (2011). Multiple cancer\/testis antigens are preferentially expressed in hormone-receptor negative and high-grade breast cancers. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017876"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1093\/annonc\/mdq325","article-title":"Cancer\u2013testis antigen expression in triple-negative breast cancer","volume":"22","author":"Curigliano","year":"2011","journal-title":"Ann. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1097\/CJI.0000000000000013","article-title":"Expression of MAGE-A3\/6 in primary breast cancer is associated with hormone receptor negative status, high histologic grade, and poor survival","volume":"37","author":"Ayyoub","year":"2014","journal-title":"J. Immunother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s10549-004-6454-7","article-title":"Prevention of metastases with a Mage-b DNA vaccine in a mouse breast tumor model: Potential for breast cancer therapy","volume":"91","author":"Sypniewska","year":"2005","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2015.07.012","article-title":"Localization of heat shock protein 110 in canine mammary gland tumors","volume":"167","author":"Okada","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1002\/eji.200737127","article-title":"Hsp110 and Grp170, members of the Hsp70 superfamily, bind to scavenger receptor-A and scavenger receptor expressed by endothelial cells-I","volume":"37","author":"Facciponte","year":"2007","journal-title":"Eur. J. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Srisawat, W., Koonyosying, P., Muenthaisong, A., Sangkakam, K., Varinrak, T., and Sthitmatee, N. (2025). Preliminary exploration of MAGE-B1, -B4, -B5, and -B10 mRNA expression in canine mammary tumors in Dogs. Animals, 15.","DOI":"10.3390\/ani15070910"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Srisawat, W., Nambooppha, B., Pringproa, K., Thongtharb, A., Prachasilchai, W., and Sthitmatee, N. (2020). A preliminary study of the cross-reactivity of canine MAGE-A with hominid monoclonal antibody 6C1 in canine mammary gland tumors: An attractive target for cancer diagnostic, prognostic and immunotherapeutic development in dogs. Vet. Sci., 7.","DOI":"10.3390\/vetsci7030109"},{"key":"ref_27","first-page":"22","article-title":"Preparation of calcium competent Escherichia coli and heat-shock transformation","volume":"1","author":"Chang","year":"2017","journal-title":"JEMI Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nmeth0106-55","article-title":"Strategies for protein coexpression in Escherichia coli","volume":"3","author":"Tolia","year":"2006","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/978-1-60761-913-0_14","article-title":"Purification of proteins fused to glutathione S-transferase","volume":"681","author":"Harper","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"A3-B","DOI":"10.1002\/0471142735.ima03bs111","article-title":"Trypan blue exclusion test of cell viability","volume":"111","author":"Strober","year":"2015","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/j.1748-5827.1996.tb01740.x","article-title":"Influence of ovariectomy at the time of mastectomy on the prognosis for canine malignant mammary tumours","volume":"37","author":"Yamagami","year":"1996","journal-title":"J. Small Anim. Pract."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jcpa.2005.05.003","article-title":"Histological grading and prognosis in dogs with mammary carcinomas: Application of a human grading method","volume":"133","author":"Karayannopoulou","year":"2005","journal-title":"J. Comp. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1177\/0300985817698208","article-title":"Prognostic significance of canine mammary tumor histologic subtypes: An observational cohort study of 229 cases","volume":"54","author":"Rasotto","year":"2017","journal-title":"Vet. Pathol."},{"key":"ref_35","first-page":"1969","article-title":"Immune responses to human tumors: Development of tumor vaccines","volume":"23","author":"Platsoucas","year":"2003","journal-title":"Anticancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ctrv.2018.04.009","article-title":"MAGE-A antigens as targets for cancer immunotherapy","volume":"67","author":"Schooten","year":"2018","journal-title":"Cancer Treat. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Alsalloum, A., Shevchenko, J.A., and Sennikov, S. (2023). The Melanoma-associated antigen family A (MAGE-A): A promising target for cancer immunotherapy?. Cancers, 15.","DOI":"10.3390\/cancers15061779"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e34506","DOI":"10.1016\/j.heliyon.2024.e34506","article-title":"Expression pattern analysis of the MAGE family genes in breast cancer patients and hypomethylation activation in the MCF-7 cells","volume":"10","author":"Almatrafi","year":"2024","journal-title":"Heliyon"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Choi, K., Jeong, H., Lee, D.H., Lee, J.W., Hong, J.E., Baek, J.E., and Park, Y.S. (2024). Innovative Cancer Immunotherapy with MAGE-A3 mRNA Cancer Vaccines. Cancers, 16.","DOI":"10.3390\/cancers16193428"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/nbt1029","article-title":"Recombinant protein folding and misfolding in Escherichia coli","volume":"22","author":"Baneyx","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1574-6968.2007.00788.x","article-title":"Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells","volume":"273","author":"Vera","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"de Marco, A., Deuerling, E., Mogk, A., Tomoyasu, T., and Bukau, B. (2007). Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. BMC Biotechnol., 7.","DOI":"10.1186\/1472-6750-7-32"},{"key":"ref_43","first-page":"1737","article-title":"Development of a recombinant HSP110-HER-2\/neu vaccine using the chaperoning properties of HSP110","volume":"62","author":"Manjili","year":"2002","journal-title":"Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/1475-2859-9-64","article-title":"Side effects of chaperone gene co-expression in recombinant protein production","volume":"9","author":"Rinas","year":"2010","journal-title":"Microb. Cell Fact."},{"key":"ref_45","first-page":"154","article-title":"Heat-shock protein fusion vectors for improved expression of soluble recombinant proteins in Escherichia coli","volume":"Volume 824","author":"Lorence","year":"2012","journal-title":"Recombinant Gene Expression"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ijbiomac.2017.04.025","article-title":"An overview on molecular chaperones enhancing solubility of expressed recombinant proteins with correct folding","volume":"102","author":"Mamipour","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0092-8674(92)90208-T","article-title":"The T cell receptor\/CD3 complex is composed of at least two autonomous transduction modules","volume":"68","author":"Wegener","year":"1992","journal-title":"Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1365-2567.2005.02137.x","article-title":"Monoclonal antibodies that identify the CD3 molecules expressed specifically at the surface of porcine \u03b3\u03b4-T cells","volume":"115","author":"Yang","year":"2005","journal-title":"Immunology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s00018-017-2686-7","article-title":"CD4 and CD8 T lymphocyte interplay in controlling tumor growth","volume":"75","author":"Ostroumov","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.molmed.2013.07.006","article-title":"Is CD69 an effective brake to control inflammatory diseases?","volume":"19","year":"2013","journal-title":"Trends Mol. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.jaci.2010.05.010","article-title":"The leukocyte activation antigen CD69 limits allergic asthma and skin contact hypersensitivity","volume":"126","author":"Lamana","year":"2010","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/j.immuni.2020.04.014","article-title":"Developmental relationships of four exhausted CD8+ T cell subsets reveals underlying transcriptional and epigenetic landscape control mechanisms","volume":"52","author":"Beltra","year":"2020","journal-title":"Immunity"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1146\/annurev-immunol-030409-101212","article-title":"Differentiation of effector CD4 T cell populations","volume":"28","author":"Zhu","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"ref_54","first-page":"925135","article-title":"CD4+ T cells: Differentiation and functions","volume":"2012","author":"Luckheeram","year":"2012","journal-title":"J. Immunol. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1038\/298859a0","article-title":"Structure of the human immune interferon gene","volume":"298","author":"Gray","year":"1982","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.4049\/jimmunol.175.6.3663","article-title":"Fusion to Listeriolysin O and delivery by Listeria monocytogenes enhances the immunogenicity of HER-2\/neu and reveals subdominant epitopes in the FVB\/N mouse","volume":"175","author":"Singh","year":"2005","journal-title":"J. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.oraloncology.2007.11.001","article-title":"CD4+ T helper responses in squamous cell carcinoma of the head and neck","volume":"44","author":"Chikamatsu","year":"2008","journal-title":"Oral Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1038\/sj.bjc.6605329","article-title":"Vaccination with Mage-b DNA induces CD8 T-cell responses at young but not old age in mice with metastatic breast cancer","volume":"101","author":"Castro","year":"2009","journal-title":"Br. J. Cancer"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5860","DOI":"10.1158\/0008-5472.CAN-08-4855","article-title":"High efficacy of a Listeria-based vaccine against metastatic breast cancer reveals a dual mode of action","volume":"69","author":"Kim","year":"2009","journal-title":"Cancer Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.imbio.2005.04.002","article-title":"HSP110 induces \u201cdanger signals\u201d upon interaction with antigen presenting cells and mouse mammary carcinoma","volume":"210","author":"Manjili","year":"2005","journal-title":"Immunobiology"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/9\/1374\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T04:15:17Z","timestamp":1778386517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/9\/1374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":60,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["ani16091374"],"URL":"https:\/\/doi.org\/10.3390\/ani16091374","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]}}}