{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:06:21Z","timestamp":1779339981092,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42306114"],"award-info":[{"award-number":["42306114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhejiang Province \u201cThree Rural Issues and Nine Parties\u201d Science and Technology Collaboration Program","award":["2026SNJF082"],"award-info":[{"award-number":["2026SNJF082"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFD2400103"],"award-info":[{"award-number":["2022YFD2400103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"\u201cPioneer\u201d and \u201cLeading Goose\u201d R&D Program of Zhejiang","award":["2024C02006"],"award-info":[{"award-number":["2024C02006"]}]},{"name":"Ningbo 2025 Major Project of Science Technology and Innovation","award":["20212003"],"award-info":[{"award-number":["20212003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Silver Pomfret is increasingly threatened by many diseases under intensive artificial culturing conditions, yet conserved host biomarkers across different infections remain poorly defined. In this study, we integrated transcriptomic datasets from independent infections with Cryptocaryon irritans, Nocardia seriolae, and Photobacterium damselae subsp. damselae to identify shared host-response genes. By combining differential expression analysis with weighted gene co-expression network analysis, we prioritized six candidate genes associated with cross-pathogen infection responses. Random Forest and support vector machine analysis further supported their classification potential across the three infection models. Phylogenetic and structural analyses provided additional evidence for the conserved annotation of these proteins. GSVA-based signature analysis supported the cross-pathogen discriminatory capacity of the six-gene panel and suggested context-dependent contributions of individual genes across infection models. Immune signature analysis indicated distinct host immune response patterns under different pathogenic challenges, and candidate genes showed positive associations with inferred T cell-related signatures. Upstream regulatory prediction identified CTCF and the miR-17\/20\/93 family as potential regulators of these genes. Quantitative real-time PCR of the kidney further highlighted canx, rnd3, and angptl4 as the most robust infection-responsive candidates, with consistent temporal expression patterns observed from 0 to 24 h post-infection. These findings suggest a potential cross-pathogen host-response pattern in Silver Pomfret and provide preliminary support for future exploration of molecular markers for disease monitoring in aquaculture.<\/jats:p>","DOI":"10.3390\/ani16101510","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:27:56Z","timestamp":1778776076000},"page":"1510","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic Analysis and Machine Learning Identify Cross-Pathogen Biomarkers for Bacterial and Parasitic Infections in Silver Pomfret (Pampus argenteus)"],"prefix":"10.3390","volume":"16","author":[{"given":"Yunkang","family":"Wu","sequence":"first","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanbo","family":"Li","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Xiangshan Aquatic Seed Industry Innovation Research Institute, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuqiang","family":"Xia","sequence":"additional","affiliation":[{"name":"Xiangshan Aquatic Seed Industry Innovation Research Institute, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4255-1594","authenticated-orcid":false,"given":"Yajun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China"},{"name":"Xiangshan Aquatic Seed Industry Innovation Research Institute, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0257-0157","authenticated-orcid":false,"given":"Xiaojun","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiabao","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China"},{"name":"Xiangshan Aquatic Seed Industry Innovation Research Institute, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131863","DOI":"10.1016\/j.ijbiomac.2024.131863","article-title":"Complement C1q Is Involved in the Activation of Membrane Attack Complexes, Regulation of Bacterial Infectious Inflammation, and Apoptosis through Overexpression in Primary Cells of Silver Pomfret (Pampus Argenteus) in Vitro","volume":"268","author":"Li","year":"2024","journal-title":"Int. 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