{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:54:05Z","timestamp":1784199245124,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Opening Foundation of Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes","award":["2022054-ZHX"],"award-info":[{"award-number":["2022054-ZHX"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Leptobotia elongata, a rare freshwater fish endemic to China, frequently exhibits retarded ovarian development under artificial captivity, severely constraining large-scale reproduction. This study evaluated the effects of combined dietary supplementation with fish oil (FO), soybean phospholipids (PLs), and vitamin E (VE) on ovarian development through a 6-month feeding trial. Hematoxylin and eosin staining revealed that oocytes in the mixed lipid group (MIX: FO + PL + VE) advanced to developmental stage III, whereas oocytes in the control group (CON) were predominantly arrested at stages I\u2013II. Transcriptomic analysis identified 1393 differentially expressed genes (651 upregulated, 742 downregulated). Upregulated genes were enriched in nucleolus- and nuclear pore-related cellular components and RNA-binding molecular functions, indicating enhanced ribosome biogenesis capacity. Downregulated genes were concentrated in DNA damage repair pathways and Mediator complex-mediated transcriptional regulation, suggesting alleviation of oxidative stress. Protein\u2013protein interaction network analysis identified mphosph10, utp11, nat10, emg1, and ftsj3 as core hub genes. Additionally, 44 differentially expressed transcription factors were identified, with Forkhead (forkhead.38), GATA (gata.4), and MYB (myb.40) significantly upregulated, indicating activation of a pro-developmental transcriptional program. These findings provide a theoretical basis for precision lipid nutrition formulation in L. elongata fish diets.<\/jats:p>","DOI":"10.3390\/ani16111604","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T11:57:44Z","timestamp":1779710264000},"page":"1604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic Mechanisms Underlying Dietary Fish Oil, Phospholipid, and Vitamin E Supplementation in Promoting Ovarian Development in Leptobotia elongata"],"prefix":"10.3390","volume":"16","author":[{"given":"Yuxin","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, China"},{"name":"Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihui","family":"Mei","sequence":"additional","affiliation":[{"name":"College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Luo","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, China"},{"name":"Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3510-9554","authenticated-orcid":false,"given":"Jian","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Guan","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, China"},{"name":"Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9676-5838","authenticated-orcid":false,"given":"Xiaojuan","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s12686-013-0021-7","article-title":"Isolation and characterization of 14 tetranucleotide microsatellite DNA markers in the elongate loach Leptobotia elongata (Cypriniformes: Cobitidae) using 454 sequencing technology","volume":"6","author":"Liu","year":"2014","journal-title":"Conserv. Genet. Resour."},{"key":"ref_2","first-page":"422","article-title":"Study on Artificial Propagation Technology of Leptobotia elongata","volume":"25","author":"Liang","year":"2001","journal-title":"Acta Hydrobiol. Sin."},{"key":"ref_3","first-page":"993","article-title":"Population parameters and resource assessment of Leptobotia elongata in the Jiangjin section of the upper Yangtze River","volume":"51","author":"Liu","year":"2016","journal-title":"Chin. J. Zool."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1002\/jsfa.8444","article-title":"Artificial propagation and embryonic development of Leptobotia elongata from Jinsha River and Yangtze River","volume":"57","author":"Zhang","year":"2018","journal-title":"Hubei Agric. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s10499-004-2478-6","article-title":"Effect of dietary lipid level on growth performance, feed utilization and body composition of grouper Epinephelus coioides juveniles fed isonitrogenous diets in floating netcages","volume":"13","author":"Luo","year":"2005","journal-title":"Aquacult. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.aninu.2024.07.003","article-title":"Dietary carbohydrate to lipid ratio affects growth, reproductive performance and health of female yellow catfish (Pelteobagrus fulvidraco): A lipidomics analysis","volume":"19","author":"Fei","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"224","DOI":"10.2108\/zsj.30.224","article-title":"Molecular cloning and transcript expression of genes encoding two types of lipoprotein lipase in the ovary of cutthroat trout, Oncorhynchus clarki","volume":"30","author":"Ryu","year":"2013","journal-title":"Zool. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mandal, S.C., Tripathy, P.S., Khatei, A., Devi, N.C., Biswas, P., Sundaray, J.K., Hoque, F., and Parhi, J. (2024). Effect of vegetable oil on ovarian steroidogenesis\u2014A transcriptome approach to understand molecular mechanisms of hypothalamus pituitary and gonad axis (HPG) in Ompok bimaculatus. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0309311"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1080\/713610925","article-title":"Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish","volume":"11","author":"Tocher","year":"2003","journal-title":"Rev. Fish. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0044-8486(01)00581-6","article-title":"Effect of fish nutrition on reproductive performance of fish","volume":"197","author":"Izquierdo","year":"2001","journal-title":"Aquaculture"},{"key":"ref_11","first-page":"100944","article-title":"De novo gonad transcriptome analysis of elongate loach (Leptobotia elongata) provides novel insights into sex-related genes","volume":"41","author":"Zhang","year":"2022","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_12","first-page":"40","article-title":"A preliminary study on the biological characteristics of Leptobotia elongata (Bleeker)","volume":"17","author":"Ku","year":"1997","journal-title":"J. Hubei Agric. Coll."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10695-025-01572-1","article-title":"Generation of donor-derived spermatozoa by cross-subfamily transplantation of purple loach (Leptobotia taeniops) spermatogonia into triploid loach (Misgurnus anguillicaudatus)","volume":"51","author":"Mei","year":"2025","journal-title":"Fish Physiol. Biochem."},{"key":"ref_14","first-page":"100722","article-title":"Thirteen years of clusterProfiler","volume":"5","author":"Yu","year":"2024","journal-title":"Innovation"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.cbpc.2006.01.002","article-title":"Vitellogenic effects of 17beta-estradiol in male Chinese loach (Misgurnus anguillicaudatus). Comparative biochemistry and physiology","volume":"143","author":"Lv","year":"2006","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/zeb.2019.1794","article-title":"Microdiet Formulation with Phospholipid Modulate Zebrafish Skeletal Development and Reproduction","volume":"17","author":"Martins","year":"2020","journal-title":"Zebrafish"},{"key":"ref_17","first-page":"6240803","article-title":"Compared to Fishmeal, Dietary Soybean Meal Improves the Reproductive Performance of Female Yellow Catfish (Pelteobagrus fulvidraco) Fish","volume":"12","author":"Chen","year":"2023","journal-title":"Aquac. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ygcen.2017.11.022","article-title":"Characterization of carp gonadotropins: Structure, annual profile, and carp and zebrafish pituitary topographic organization","volume":"264","author":"Golan","year":"2018","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Li, Y., Liang, X., Cao, X., Huang, L., Yan, J., Wei, Y., and Gao, J. (2017). Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0172386"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cbpb.2015.12.002","article-title":"Identification and structural characterization of two peroxisome proliferator activated receptors and their transcriptional changes at different developmental stages and after feeding with different fatty acids","volume":"193","author":"Liang","year":"2016","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1093\/biolre\/ioae009","article-title":"IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis","volume":"110","author":"Li","year":"2024","journal-title":"Biol. Reprod."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Song, Y.F., Tan, X.Y., Pan, Y.X., Zhang, L.H., and Chen, Q.L. (2018). Fatty Acid beta-Oxidation Is Essential in Leptin-Mediated Oocytes Maturation of Yellow Catfish Pelteobagrus fulvidraco. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051457"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"144350","DOI":"10.1016\/j.gene.2020.144350","article-title":"Identification, expression profiling and localization of thoc in common carp ovary: Influence of thoc3-siRNA transient silencing","volume":"732","author":"Gupta","year":"2020","journal-title":"Gene"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Shah, A.N., Leesch, F., Lorenzo-Orts, L., Grundmann, L., Novatchkova, M., Haselbach, D., Calo, E., and Pauli, A. (2024). A dual ribosomal system in the zebrafish soma and germline. bioRxiv.","DOI":"10.1101\/2024.08.29.610041"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1016\/j.ygeno.2020.01.026","article-title":"De novo transcriptome assembly and sex-biased gene expression in the gonads of Amur catfish (Silurus asotus)","volume":"112","author":"Shen","year":"2020","journal-title":"Genomics"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Eldem, V., Zararsiz, G., and Erkan, M. (2023). Global expression pattern of genes containing positively selected sites in European anchovy (Engraulis encrasicolus L.) may shed light on teleost reproduction. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0289940"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16017","DOI":"10.3390\/ijms160716017","article-title":"Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus","volume":"16","author":"Luo","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_28","first-page":"1069","article-title":"Expression profile of ribosomal protein L10a throughout gonadal development in rainbow trout (Oncorhynchus mykiss)","volume":"40","author":"Makkapan","year":"2014","journal-title":"Fish Physiol. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kuang, G., Tao, W., Zheng, S., Wang, X., and Wang, D. (2020). Genome-Wide Identification, Evolution and Expression of the Complete Set of Cytoplasmic Ribosomal Protein Genes in Nile Tilapia. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21041230"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5248","DOI":"10.1038\/s41467-024-49613-2","article-title":"Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios","volume":"15","author":"Wilson","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, C., Chen, X., Dai, Y., Zhang, Y., Sun, Y., and Cui, X. (2022). Comparative transcriptome analysis of heat-induced domesticated zebrafish during gonadal differentiation. BMC Genom. Data, 23.","DOI":"10.1186\/s12863-022-01058-6"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.scib.2023.04.029","article-title":"An efficient approach to synthesize sterile allopolyploids through the combined reproduction mode of ameiotic oogenesis and sperm-egg fusion in the polyploid Carassius complex","volume":"68","author":"Lu","year":"2023","journal-title":"Sci. Bull."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s10695-022-01067-3","article-title":"Cloning and expressions of chop in loach (Misgurnus anguillicaudatus) and its response to hydrogen peroxide (H2O2) stress","volume":"48","author":"Li","year":"2022","journal-title":"Fish Physiol. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ydbio.2013.10.023","article-title":"Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development","volume":"385","author":"Wu","year":"2014","journal-title":"Dev. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e100204","DOI":"10.7554\/eLife.100204","article-title":"Single-cell transcriptomes of zebrafish germline reveal progenitor types and feminization by Foxl2l","volume":"14","author":"Hsu","year":"2025","journal-title":"eLife"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Chen, F., Zhong, C.C., Song, C.C., Chen, S.W., He, Y., and Tan, X.Y. (2021). Molecular Characterization and Functional Analysis of Two Steroidogenic Genes TSPO and SMAD4 in Yellow Catfish. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094505"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"123794","DOI":"10.1016\/j.ijbiomac.2023.123794","article-title":"DNA methylation mediates gonadal development via regulating the expression levels of cyp19a1a in loach Misgurnus anguillicaudatus","volume":"235","author":"Su","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"107661","DOI":"10.1016\/j.anireprosci.2024.107661","article-title":"Gonadal expression profiles reveal the underlying mechanisms of temperature effects on sex determination in the large-scale loach (Paramisgurnus dabryanus)","volume":"272","author":"Zhang","year":"2025","journal-title":"Anim. Reprod. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s10709-018-0039-6","article-title":"Cloning, characterization and function analysis of DAX1 in Chinese loach (Paramisgurnus dabryanus)","volume":"146","author":"Xia","year":"2018","journal-title":"Genetica"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Qiu, Y., Sun, S., Charkraborty, T., Wu, L., Sun, L., Wei, J., Nagahama, Y., Wang, D., and Zhou, L. (2015). Figla Favors Ovarian Differentiation by Antagonizing Spermatogenesis in a Teleosts, Nile Tilapia (Oreochromis niloticus). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0123900"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hou, M., Zhang, J., Wang, Q., Zhao, R., Cao, Y., Chen, Y., Wang, K., Ding, N., Qi, Y., and Sun, X. (2024). Single-Nucleus RNA Sequencing Reveals the Transcriptome Profiling of Ovarian Cells in Adolescent Cyprinus carpio. Animals, 14.","DOI":"10.3390\/ani14223263"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/11\/1604\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T12:18:05Z","timestamp":1779711485000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/11\/1604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,25]]},"references-count":41,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ani16111604"],"URL":"https:\/\/doi.org\/10.3390\/ani16111604","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,25]]}}}