{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:30:03Z","timestamp":1785407403672,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32502872"],"award-info":[{"award-number":["32502872"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32502872"],"award-info":[{"award-number":["32502872"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Key Projects of Scientific Research Plan of Colleges and Universities of Anhui Province","award":["2025AHGXZK30137"],"award-info":[{"award-number":["2025AHGXZK30137"]}]},{"name":"Scientific research project of Fuyang Normal University","award":["2024KYQD0149"],"award-info":[{"award-number":["2024KYQD0149"]}]},{"name":"Scientific research project of Fuyang Normal University","award":["2025KYQD0104"],"award-info":[{"award-number":["2025KYQD0104"]}]},{"award":["2024KYQD0149"],"award-info":[{"award-number":["2024KYQD0149"]}],"id":[{"id":"https:\/\/ror.org\/02njz9p87","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025KYQD0104"],"award-info":[{"award-number":["2025KYQD0104"]}],"id":[{"id":"https:\/\/ror.org\/02njz9p87","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The corpus luteum number in sows is a key reproductive indicator for measuring ovulation rate and reproductive efficiency. Its formation is precisely regulated by a complex gene regulatory network composed of multi-level molecular interactions. To systematically elucidate the molecular basis of this trait, this study comprehensively analyzed genes located within QTL for corpus luteum number. This approach identified a series of key regulatory molecules specifically expressed in the ovary, including transcription factors (TFs), long non-coding RNAs (lncRNAs), and microRNAs (miRNAs). Using bioinformatics methods to predict the target genes of candidate miRNAs, combined with functional enrichment analysis, revealed that these target genes were significantly enriched in multiple core reproductive pathways closely related to cell proliferation, differentiation, and hormone regulation, including the ErbB signaling pathway, PI3K-Akt signaling pathway, and TGF-beta signaling pathway. Based on the above findings, this study ultimately constructed a TF-lncRNA-miRNA-mRNA network, which is associated with QTL for corpus luteum number. Furthermore, key genes were validated via quantitative real-time PCR (qRT-PCR). Significant positive correlations were identified between the transcription factor NEUROG2 and lncRNA LOC102167554, along with its potential target gene ESRP1, as well as between transcription factor SNAI2 and lncRNAs (LOC102167554, LOC102167796) and their potential target genes (FXR, ERBB4). In addition, the functional validation results showed that the interference of LOC102167554 significantly reduced the proliferation ability of sGCs. These key genes represent potential targets for genetic improvement of sow reproduction.<\/jats:p>","DOI":"10.3390\/ani16111693","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:09:16Z","timestamp":1780301356000},"page":"1693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction of TF-lncRNA-miRNA-mRNA Regulatory Network Affecting Sow Reproduction Based on QTLs for Corpus Luteum Number"],"prefix":"10.3390","volume":"16","author":[{"given":"Miaomiao","family":"Wang","sequence":"first","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Lu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Gao","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxu","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengteng","family":"Xu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Yang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0583-072X","authenticated-orcid":false,"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2404747","DOI":"10.1002\/advs.202404747","article-title":"A Mutation Losing an RBP-Binding Site in the LncRNA NORSF Transcript Influences Granulosa Cell Apoptosis and Sow Fertility","volume":"11","author":"Wang","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e96","DOI":"10.1093\/jas\/skae096","article-title":"Effects of dietary iron supplementation on reproductive performance of sows and growth performance of piglets","volume":"102","author":"Xiong","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1007\/s00484-022-02365-4","article-title":"Heat wave exposure impairs reproductive performance in primiparous sows and gilts in a tropical environment","volume":"66","author":"Brito","year":"2022","journal-title":"Int. J. Biometeorol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1038\/s41419-024-07286-1","article-title":"miR-184, a downregulated ovary-elevated miRNA transcriptionally activated by SREBF2, exerts anti-apoptotic properties in ovarian granulosa cells through inducing SMAD3 expression","volume":"15","author":"Shan","year":"2024","journal-title":"Cell Death Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/s41419-025-08097-8","article-title":"The MYC-dependent lncRNA MB3 inhibits apoptosis in Group 3 Medulloblastoma by regulating the TGF-\u03b2 pathway via HMGN5","volume":"16","author":"Grandioso","year":"2025","journal-title":"Cell Death Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1016\/j.molcel.2025.03.006","article-title":"An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation","volume":"85","author":"Lee","year":"2025","journal-title":"Mol. Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.molcel.2025.12.017","article-title":"ICAM1 mRNA entraps ILF2\/ILF3 to inhibit transcription of EIF4E and global protein synthesis","volume":"86","author":"Jiang","year":"2026","journal-title":"Mol. Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/s41388-024-03225-8","article-title":"LINC00882, transcriptionally activated by CEBP-\u03b2 and post-transcriptionally stabilized by METTL14-mediated m6A modification, exerts tumorigenesis by promoting PABPC1-mediated stabilization of ELK3 mRNA","volume":"44","author":"Gao","year":"2025","journal-title":"Oncogene"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/s40104-021-00626-7","article-title":"NORHA, a novel follicular atresia-related lncRNA, promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis","volume":"12","author":"Yao","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Duan, S., Li, F., Meng, L., Cheng, S., Shi, H., Qu, Y., He, C., Gao, S., Li, J., and Sun, S. (2025). Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size. Front. Genet., 16.","DOI":"10.3389\/fgene.2025.1498076"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/s42003-020-0864-x","article-title":"NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death","volume":"3","author":"Du","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"f1018","DOI":"10.1093\/nar\/gkaf1018","article-title":"Functional microRNA targeting without seed pairing","volume":"53","author":"Hall","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4238","DOI":"10.1002\/jcp.30872","article-title":"Lnc2300 is a cis-acting long noncoding RNA of CYP11A1 in ovarian granulosa cells","volume":"237","author":"Wang","year":"2022","journal-title":"J. Cell. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137401","DOI":"10.1016\/j.ijbiomac.2024.137401","article-title":"The splicing factor SF3B1 is essential for proper alternative splicing and zygotic genome activation in early porcine embryos","volume":"282","author":"Zhao","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e71445","DOI":"10.1096\/fj.202503270R","article-title":"hnRNPU Safeguards Oocyte Development and Female Fertility via Regulation of Alternative Splicing","volume":"40","author":"Li","year":"2026","journal-title":"FASEB J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s41586-025-08916-0","article-title":"Low overlap of transcription factor DNA binding and regulatory targets","volume":"642","author":"Mahendrawada","year":"2025","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/j.molcel.2025.03.004","article-title":"Widespread variation in molecular interactions and regulatory properties among transcription factor isoforms","volume":"85","author":"Lambourne","year":"2025","journal-title":"Mol. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wang, M., Wang, Y., Yang, L., Du, X., and Li, Q. (2023). Nuclear lncRNA NORSF reduces E2 release in granulosa cells by sponging the endogenous small activating RNA miR-339. BMC Biol., 21.","DOI":"10.1186\/s12915-023-01731-x"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13048-021-00802-3","article-title":"Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth","volume":"14","author":"Toms","year":"2021","journal-title":"J. Ovarian Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"107728","DOI":"10.1016\/j.phrs.2025.107728","article-title":"Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD","volume":"215","author":"Tang","year":"2025","journal-title":"Pharmacol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3715","DOI":"10.1038\/s41467-021-23560-8","article-title":"A pig BodyMap transcriptome reveals diverse tissue physiologies and evolutionary dynamics of transcription","volume":"12","author":"Jin","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/s41593-021-01002-4","article-title":"Multimodal profiling of the transcriptional regulatory landscape of the developing mouse cortex identifies Neurog2 as a key epigenome remodeler","volume":"25","author":"Noack","year":"2022","journal-title":"Nat. Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8362","DOI":"10.1038\/s41467-023-43602-7","article-title":"Essential transcription factors for induced neuron differentiation","volume":"14","author":"Lu","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1007\/s10528-023-10546-y","article-title":"SNAI2\/FTH1P3\/miR-218-5p Positive Feedback Loop Promotes Colorectal Cancer Metastasis","volume":"62","author":"Deng","year":"2024","journal-title":"Biochem. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"140697","DOI":"10.1016\/j.jhazmat.2025.140697","article-title":"Tiam1 up-regulation by long non-coding RNA ABHD11-AS1 sponging of miR-182-5p causes \u03b2-catenin pathway activation to promote hexavalent chromium lung carcinogenesis","volume":"501","author":"Bi","year":"2026","journal-title":"J. Hazard. Mater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s13578-023-01024-4","article-title":"ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1","volume":"13","author":"Chen","year":"2023","journal-title":"Cell Biosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e37","DOI":"10.1093\/molehr\/gaae037","article-title":"Interleukin-22 improves ovulation in polycystic ovary syndrome via STAT3 signaling","volume":"30","author":"Liao","year":"2024","journal-title":"Mol. Hum. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/s40168-025-02174-y","article-title":"Gut microbiota-derived short-chain fatty acids promote follicular maturation via gWAT-ovary axis in mammals","volume":"13","author":"Xu","year":"2025","journal-title":"Microbiome"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/10495398.2011.630897","article-title":"Association of the porcine transforming growth factor beta type I receptor (TGFBR1) gene with growth and carcass traits","volume":"23","author":"Chen","year":"2012","journal-title":"Anim. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3792","DOI":"10.2527\/jas.2014-7788","article-title":"Genomewide association and identification of candidate genes for ovulation rate in swine","volume":"92","author":"Schneider","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"722","DOI":"10.24272\/j.issn.2095-8137.2024.378","article-title":"HNRNPA2B1-mediated m6A modification enhances lncRNA NORHA stability to control granulosa cell functions","volume":"46","author":"Zhou","year":"2025","journal-title":"Zool. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e31414","DOI":"10.1002\/jcp.31414","article-title":"A variant in long noncoding RNA NORSF affects granulosa cells response to transcription factor RFX7","volume":"239","author":"Wang","year":"2024","journal-title":"J. Cell. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"134837","DOI":"10.1016\/j.ijbiomac.2024.134837","article-title":"LncRNA BC200 promotes the development of EBV-associated nasopharyngeal carcinoma by competitively binding to miR-6834-5p to upregulate TYMS expression","volume":"278","author":"Zhang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1158\/1078-0432.CCR-07-1731","article-title":"MicroRNA expression profiles in serous ovarian carcinoma","volume":"14","author":"Nam","year":"2008","journal-title":"Clin. Cancer Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1038\/s41598-017-02225-x","article-title":"Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing","volume":"7","author":"Zi","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s12958-020-00590-3","article-title":"hsa-miR-100-5p, an overexpressed miRNA in human ovarian endometriotic stromal cells, promotes invasion through attenuation of SMARCD1 expression","volume":"18","author":"Takebayashi","year":"2020","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/00071668.2022.2159788","article-title":"miR-199-3p suppresses cellular migration and viability and promotes progesterone production in goose ovarian follicles before selection through regulating ITGB8 and other ECM-related genes","volume":"64","author":"Li","year":"2023","journal-title":"Br. Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"16257","DOI":"10.1073\/pnas.0508521102","article-title":"Epidermal growth factor receptor signaling is required for normal ovarian steroidogenesis and oocyte maturation","volume":"102","author":"Jamnongjit","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41419-023-05759-3","article-title":"FHL2 deficiency impairs follicular development and fertility by attenuating EGF\/EGFR\/YAP signaling in ovarian granulosa cells","volume":"14","author":"Wang","year":"2023","journal-title":"Cell Death Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1021\/acs.jafc.8b06361","article-title":"Ochratoxin A Exposure Impairs Porcine Granulosa Cell Growth via the PI3K\/AKT Signaling Pathway","volume":"67","author":"Zhang","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"117597","DOI":"10.1016\/j.theriogenology.2025.117597","article-title":"GAS1 affects proliferation, apoptosis, and steroid hormone levels by regulating mitochondrial functions and the PI3K\/AKT pathway in bovine granulosa cells","volume":"248","author":"Yu","year":"2025","journal-title":"Theriogenology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.theriogenology.2017.05.022","article-title":"BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-\u03b2 signaling","volume":"100","author":"Bai","year":"2017","journal-title":"Theriogenology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/s41419-017-0205-2","article-title":"SMAD4 feedback regulates the canonical TGF-\u03b2 signaling pathway to control granulosa cell apoptosis","volume":"9","author":"Du","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1530\/rep.1.01074","article-title":"TGF-\u03b2 superfamily members and ovarian follicle development","volume":"132","author":"Knight","year":"2006","journal-title":"Reproduction"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.immuni.2025.11.009","article-title":"The TCR-SUB1-DOCK2 axis promotes autoimmunity by driving pathogenic CD4+ T cell tissue infiltration","volume":"59","author":"Li","year":"2026","journal-title":"Immunity"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1262\/jrd.2018-054","article-title":"Evidence for the involvement of FXR signaling in ovarian granulosa cell function","volume":"65","author":"Takae","year":"2019","journal-title":"J. Reprod. Dev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1093\/humrep\/dei162","article-title":"Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum","volume":"20","author":"Akayama","year":"2005","journal-title":"Hum. Reprod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/S1050-1738(01)00065-2","article-title":"Erbb4 and its isoforms. selective regulation of growth factor responses by naturally occurring receptor variants","volume":"10","author":"Junttila","year":"2000","journal-title":"Trends Cardiovasc. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jare.2023.06.002","article-title":"Retinoic acid enhances ovarian steroidogenesis by regulating granulosa cell proliferation and MESP2\/STAR\/CYP11A1 pathway","volume":"58","author":"Cai","year":"2024","journal-title":"J. Adv. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev-biochem-060308-103103","article-title":"Regulation of mRNA translation and stability by microRNAs","volume":"79","author":"Fabian","year":"2010","journal-title":"Annu. Rev. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e389","DOI":"10.1038\/oncsis.2017.87","article-title":"ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells","volume":"6","author":"Jeong","year":"2017","journal-title":"Oncogenesis"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"10007","DOI":"10.18632\/oncotarget.14318","article-title":"The RNA-binding protein ESRP1 promotes human colorectal cancer progression","volume":"8","author":"Fagoonee","year":"2017","journal-title":"Oncotarget"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/11\/1693\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:26:41Z","timestamp":1780302401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/11\/1693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":52,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ani16111693"],"URL":"https:\/\/doi.org\/10.3390\/ani16111693","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]}}}